Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS07530 and RALBFv3_RS07535 are separated by 67 nucleotides RALBFv3_RS07535 and RALBFv3_RS07540 are separated by 15 nucleotides
RALBFv3_RS07530: RALBFv3_RS07530 - NAD(P)/FAD-dependent oxidoreductase, at 1,657,245 to 1,658,858
_RS07530
RALBFv3_RS07535: RALBFv3_RS07535 - short-chain dehydrogenase, at 1,658,926 to 1,659,816
_RS07535
RALBFv3_RS07540: RALBFv3_RS07540 - alpha/beta hydrolase, at 1,659,832 to 1,660,887
_RS07540
Position (kb)
1658
1659
1660 Strain fitness (log2 ratio)
-1
0
1 at 1658.025 kb on + strand, within RALBFv3_RS07530 at 1658.025 kb on + strand, within RALBFv3_RS07530 at 1658.026 kb on - strand, within RALBFv3_RS07530 at 1658.026 kb on - strand, within RALBFv3_RS07530 at 1658.161 kb on - strand, within RALBFv3_RS07530 at 1658.161 kb on - strand, within RALBFv3_RS07530 at 1658.199 kb on + strand, within RALBFv3_RS07530 at 1658.199 kb on + strand, within RALBFv3_RS07530 at 1658.200 kb on - strand, within RALBFv3_RS07530 at 1658.200 kb on - strand, within RALBFv3_RS07530 at 1658.200 kb on - strand, within RALBFv3_RS07530 at 1658.200 kb on - strand, within RALBFv3_RS07530 at 1658.202 kb on + strand, within RALBFv3_RS07530 at 1658.202 kb on + strand, within RALBFv3_RS07530 at 1658.419 kb on - strand, within RALBFv3_RS07530 at 1658.419 kb on - strand, within RALBFv3_RS07530 at 1658.419 kb on - strand, within RALBFv3_RS07530 at 1658.419 kb on - strand, within RALBFv3_RS07530 at 1658.526 kb on + strand, within RALBFv3_RS07530 at 1658.527 kb on - strand at 1658.527 kb on - strand, within RALBFv3_RS07530 at 1659.064 kb on + strand, within RALBFv3_RS07535 at 1659.065 kb on + strand, within RALBFv3_RS07535 at 1659.065 kb on + strand, within RALBFv3_RS07535 at 1659.066 kb on - strand, within RALBFv3_RS07535 at 1659.397 kb on + strand, within RALBFv3_RS07535 at 1659.397 kb on + strand, within RALBFv3_RS07535 at 1659.397 kb on + strand, within RALBFv3_RS07535 at 1659.398 kb on - strand, within RALBFv3_RS07535 at 1659.412 kb on + strand, within RALBFv3_RS07535 at 1659.412 kb on + strand, within RALBFv3_RS07535 at 1659.428 kb on - strand, within RALBFv3_RS07535 at 1659.815 kb on - strand at 1660.320 kb on - strand, within RALBFv3_RS07540 at 1660.343 kb on - strand, within RALBFv3_RS07540 at 1660.354 kb on + strand, within RALBFv3_RS07540 at 1660.421 kb on - strand, within RALBFv3_RS07540 at 1660.511 kb on - strand, within RALBFv3_RS07540 at 1660.553 kb on - strand, within RALBFv3_RS07540 at 1660.568 kb on - strand, within RALBFv3_RS07540 at 1660.568 kb on - strand, within RALBFv3_RS07540 at 1660.568 kb on - strand, within RALBFv3_RS07540
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,658,025 + RALBFv3_RS07530 0.48 -1.3 1,658,025 + RALBFv3_RS07530 0.48 -0.1 1,658,026 - RALBFv3_RS07530 0.48 -1.2 1,658,026 - RALBFv3_RS07530 0.48 -0.2 1,658,161 - RALBFv3_RS07530 0.57 +0.2 1,658,161 - RALBFv3_RS07530 0.57 +0.8 1,658,199 + RALBFv3_RS07530 0.59 -0.2 1,658,199 + RALBFv3_RS07530 0.59 -0.1 1,658,200 - RALBFv3_RS07530 0.59 -0.8 1,658,200 - RALBFv3_RS07530 0.59 -0.2 1,658,200 - RALBFv3_RS07530 0.59 +0.5 1,658,200 - RALBFv3_RS07530 0.59 +1.2 1,658,202 + RALBFv3_RS07530 0.59 -0.1 1,658,202 + RALBFv3_RS07530 0.59 -1.0 1,658,419 - RALBFv3_RS07530 0.73 +1.2 1,658,419 - RALBFv3_RS07530 0.73 +1.2 1,658,419 - RALBFv3_RS07530 0.73 +0.0 1,658,419 - RALBFv3_RS07530 0.73 +0.6 1,658,526 + RALBFv3_RS07530 0.79 -0.1 1,658,527 - +1.2 1,658,527 - RALBFv3_RS07530 0.79 +0.0 1,659,064 + RALBFv3_RS07535 0.15 -0.2 1,659,065 + RALBFv3_RS07535 0.16 -0.1 1,659,065 + RALBFv3_RS07535 0.16 -0.7 1,659,066 - RALBFv3_RS07535 0.16 +0.6 1,659,397 + RALBFv3_RS07535 0.53 -0.6 1,659,397 + RALBFv3_RS07535 0.53 +0.3 1,659,397 + RALBFv3_RS07535 0.53 +0.0 1,659,398 - RALBFv3_RS07535 0.53 +0.0 1,659,412 + RALBFv3_RS07535 0.55 +0.6 1,659,412 + RALBFv3_RS07535 0.55 +0.2 1,659,428 - RALBFv3_RS07535 0.56 -0.0 1,659,815 - -1.2 1,660,320 - RALBFv3_RS07540 0.46 +1.2 1,660,343 - RALBFv3_RS07540 0.48 -0.4 1,660,354 + RALBFv3_RS07540 0.49 +0.2 1,660,421 - RALBFv3_RS07540 0.56 -0.2 1,660,511 - RALBFv3_RS07540 0.64 -0.1 1,660,553 - RALBFv3_RS07540 0.68 +0.0 1,660,568 - RALBFv3_RS07540 0.70 +1.2 1,660,568 - RALBFv3_RS07540 0.70 +1.2 1,660,568 - RALBFv3_RS07540 0.70 +0.2
Or see this region's nucleotide sequence