Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS24710

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS22180 and RALBFv3_RS24710 are separated by 110 nucleotidesRALBFv3_RS24710 and RALBFv3_RS22185 overlap by 18 nucleotides RALBFv3_RS22180: RALBFv3_RS22180 - serine hydrolase, at 1,719,363 to 1,720,718 _RS22180 RALBFv3_RS24710: RALBFv3_RS24710 - hypothetical protein, at 1,720,829 to 1,721,083 _RS24710 RALBFv3_RS22185: RALBFv3_RS22185 - glucan biosynthesis protein D, at 1,721,066 to 1,722,679 _RS22185 Position (kb) 1720 1721 1722Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1719.943 kb on + strand, within RALBFv3_RS22180at 1719.943 kb on + strand, within RALBFv3_RS22180at 1719.944 kb on - strand, within RALBFv3_RS22180at 1720.072 kb on + strand, within RALBFv3_RS22180at 1720.120 kb on + strand, within RALBFv3_RS22180at 1720.120 kb on + strand, within RALBFv3_RS22180at 1720.121 kb on - strand, within RALBFv3_RS22180at 1720.377 kb on - strand, within RALBFv3_RS22180at 1720.377 kb on - strand, within RALBFv3_RS22180at 1720.748 kb on + strandat 1720.749 kb on - strandat 1720.749 kb on - strandat 1720.826 kb on + strandat 1720.826 kb on + strandat 1720.827 kb on - strandat 1720.830 kb on + strandat 1720.831 kb on - strandat 1720.831 kb on - strandat 1720.831 kb on - strandat 1721.020 kb on + strand, within RALBFv3_RS24710at 1721.020 kb on + strand, within RALBFv3_RS24710at 1721.020 kb on + strand, within RALBFv3_RS24710at 1721.021 kb on - strandat 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.021 kb on - strand, within RALBFv3_RS24710at 1721.050 kb on + strand, within RALBFv3_RS24710at 1721.051 kb on - strand, within RALBFv3_RS24710at 1721.051 kb on - strand, within RALBFv3_RS24710at 1721.051 kb on - strand, within RALBFv3_RS24710at 1721.234 kb on + strand, within RALBFv3_RS22185at 1721.234 kb on + strand, within RALBFv3_RS22185at 1721.235 kb on - strand, within RALBFv3_RS22185at 1721.283 kb on - strand, within RALBFv3_RS22185at 1721.451 kb on - strand, within RALBFv3_RS22185at 1721.451 kb on - strand, within RALBFv3_RS22185at 1721.597 kb on + strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.622 kb on - strand, within RALBFv3_RS22185at 1721.627 kb on + strand, within RALBFv3_RS22185at 1721.627 kb on + strand, within RALBFv3_RS22185at 1721.627 kb on + strand, within RALBFv3_RS22185at 1721.628 kb on - strand, within RALBFv3_RS22185at 1721.753 kb on + strand, within RALBFv3_RS22185at 1721.753 kb on + strand, within RALBFv3_RS22185at 1721.897 kb on + strand, within RALBFv3_RS22185at 1721.897 kb on + strand, within RALBFv3_RS22185at 1721.898 kb on - strand, within RALBFv3_RS22185

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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1,719,943 + RALBFv3_RS22180 0.43 +0.4
1,719,943 + RALBFv3_RS22180 0.43 +1.5
1,719,944 - RALBFv3_RS22180 0.43 +0.1
1,720,072 + RALBFv3_RS22180 0.52 +0.3
1,720,120 + RALBFv3_RS22180 0.56 +0.2
1,720,120 + RALBFv3_RS22180 0.56 +0.5
1,720,121 - RALBFv3_RS22180 0.56 +0.5
1,720,377 - RALBFv3_RS22180 0.75 -1.1
1,720,377 - RALBFv3_RS22180 0.75 +1.5
1,720,748 + -0.4
1,720,749 - +0.2
1,720,749 - +0.1
1,720,826 + +1.5
1,720,826 + +0.3
1,720,827 - -0.4
1,720,830 + +0.3
1,720,831 - +0.0
1,720,831 - +0.5
1,720,831 - -0.5
1,721,020 + RALBFv3_RS24710 0.75 +0.6
1,721,020 + RALBFv3_RS24710 0.75 -0.1
1,721,020 + RALBFv3_RS24710 0.75 +0.2
1,721,021 - +1.5
1,721,021 - RALBFv3_RS24710 0.75 +2.1
1,721,021 - RALBFv3_RS24710 0.75 -0.5
1,721,021 - RALBFv3_RS24710 0.75 -0.8
1,721,021 - RALBFv3_RS24710 0.75 -0.4
1,721,021 - RALBFv3_RS24710 0.75 +0.9
1,721,050 + RALBFv3_RS24710 0.87 +1.5
1,721,051 - RALBFv3_RS24710 0.87 -1.4
1,721,051 - RALBFv3_RS24710 0.87 +0.4
1,721,051 - RALBFv3_RS24710 0.87 +0.5
1,721,234 + RALBFv3_RS22185 0.10 -2.9
1,721,234 + RALBFv3_RS22185 0.10 -1.0
1,721,235 - RALBFv3_RS22185 0.10 -1.9
1,721,283 - RALBFv3_RS22185 0.13 -0.5
1,721,451 - RALBFv3_RS22185 0.24 -2.1
1,721,451 - RALBFv3_RS22185 0.24 +1.5
1,721,597 + RALBFv3_RS22185 0.33 -2.1
1,721,622 - RALBFv3_RS22185 0.34 -2.0
1,721,622 - RALBFv3_RS22185 0.34 -1.5
1,721,622 - RALBFv3_RS22185 0.34 -2.1
1,721,622 - RALBFv3_RS22185 0.34 -2.2
1,721,627 + RALBFv3_RS22185 0.35 -1.9
1,721,627 + RALBFv3_RS22185 0.35 +1.5
1,721,627 + RALBFv3_RS22185 0.35 -1.7
1,721,628 - RALBFv3_RS22185 0.35 -1.8
1,721,753 + RALBFv3_RS22185 0.43 -5.2
1,721,753 + RALBFv3_RS22185 0.43 -0.5
1,721,897 + RALBFv3_RS22185 0.51 +0.5
1,721,897 + RALBFv3_RS22185 0.51 -0.5
1,721,898 - RALBFv3_RS22185 0.52 -3.5

Or see this region's nucleotide sequence