Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10440

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntRALBFv3_RS10430 and RALBFv3_RS10435 are separated by 19 nucleotidesRALBFv3_RS10435 and RALBFv3_RS10440 are separated by 1 nucleotidesRALBFv3_RS10440 and RALBFv3_RS10445 overlap by 2 nucleotidesRALBFv3_RS10445 and RALBFv3_RS10450 are separated by 78 nucleotidesRALBFv3_RS10450 and RALBFv3_RS10455 overlap by 4 nucleotides RALBFv3_RS10430: RALBFv3_RS10430 - YbaK/EbsC family protein, at 2,321,855 to 2,322,358 _RS10430 RALBFv3_RS10435: RALBFv3_RS10435 - hydroxymethylglutaryl-CoA lyase, at 2,322,378 to 2,323,307 _RS10435 RALBFv3_RS10440: RALBFv3_RS10440 - glyoxylate/hydroxypyruvate reductase A, at 2,323,309 to 2,324,250 _RS10440 RALBFv3_RS10445: RALBFv3_RS10445 - hypothetical protein, at 2,324,249 to 2,324,524 _RS10445 RALBFv3_RS10450: RALBFv3_RS10450 - hypothetical protein, at 2,324,603 to 2,324,887 _RS10450 RALBFv3_RS10455: RALBFv3_RS10455 - DUF2778 domain-containing protein, at 2,324,884 to 2,325,306 _RS10455 Position (kb) 2323 2324 2325Strain fitness (log2 ratio) -3 -2 -1 0 1at 2322.383 kb on + strandat 2322.384 kb on - strandat 2322.384 kb on - strandat 2322.538 kb on + strand, within RALBFv3_RS10435at 2322.539 kb on - strand, within RALBFv3_RS10435at 2322.539 kb on - strand, within RALBFv3_RS10435at 2322.673 kb on + strand, within RALBFv3_RS10435at 2322.673 kb on + strand, within RALBFv3_RS10435at 2322.674 kb on - strand, within RALBFv3_RS10435at 2322.823 kb on - strand, within RALBFv3_RS10435at 2322.859 kb on + strand, within RALBFv3_RS10435at 2323.078 kb on + strand, within RALBFv3_RS10435at 2323.079 kb on - strand, within RALBFv3_RS10435at 2323.586 kb on + strand, within RALBFv3_RS10440at 2323.587 kb on - strand, within RALBFv3_RS10440at 2323.587 kb on - strand, within RALBFv3_RS10440at 2323.797 kb on + strand, within RALBFv3_RS10440at 2323.798 kb on - strand, within RALBFv3_RS10440at 2323.899 kb on - strand, within RALBFv3_RS10440at 2323.944 kb on - strand, within RALBFv3_RS10440at 2323.944 kb on - strand, within RALBFv3_RS10440at 2324.129 kb on + strand, within RALBFv3_RS10440at 2324.129 kb on + strand, within RALBFv3_RS10440at 2324.234 kb on + strandat 2324.235 kb on - strandat 2324.235 kb on - strandat 2324.235 kb on - strandat 2324.290 kb on + strand, within RALBFv3_RS10445at 2324.525 kb on + strandat 2324.526 kb on - strandat 2324.604 kb on + strandat 2324.604 kb on + strandat 2324.605 kb on - strandat 2324.605 kb on - strandat 2324.634 kb on - strand, within RALBFv3_RS10450at 2324.667 kb on - strand, within RALBFv3_RS10450at 2324.694 kb on + strand, within RALBFv3_RS10450at 2324.695 kb on - strand, within RALBFv3_RS10450at 2324.695 kb on - strand, within RALBFv3_RS10450at 2324.695 kb on - strand, within RALBFv3_RS10450at 2324.811 kb on + strand, within RALBFv3_RS10450at 2324.812 kb on - strand, within RALBFv3_RS10450at 2324.852 kb on + strand, within RALBFv3_RS10450at 2324.852 kb on + strand, within RALBFv3_RS10450at 2324.852 kb on + strand, within RALBFv3_RS10450at 2324.852 kb on + strand, within RALBFv3_RS10450at 2324.853 kb on - strand, within RALBFv3_RS10450at 2324.853 kb on - strand, within RALBFv3_RS10450at 2324.876 kb on + strandat 2324.877 kb on - strandat 2325.076 kb on - strand, within RALBFv3_RS10455at 2325.174 kb on + strand, within RALBFv3_RS10455at 2325.185 kb on + strand, within RALBFv3_RS10455at 2325.185 kb on + strand, within RALBFv3_RS10455at 2325.186 kb on - strand, within RALBFv3_RS10455at 2325.186 kb on - strand, within RALBFv3_RS10455at 2325.186 kb on - strand, within RALBFv3_RS10455at 2325.188 kb on + strand, within RALBFv3_RS10455at 2325.188 kb on + strand, within RALBFv3_RS10455at 2325.188 kb on + strand, within RALBFv3_RS10455at 2325.189 kb on - strand, within RALBFv3_RS10455at 2325.189 kb on - strand, within RALBFv3_RS10455at 2325.189 kb on - strand, within RALBFv3_RS10455at 2325.189 kb on - strand, within RALBFv3_RS10455

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
remove
2,322,383 + -0.1
2,322,384 - -0.3
2,322,384 - +0.4
2,322,538 + RALBFv3_RS10435 0.17 -1.1
2,322,539 - RALBFv3_RS10435 0.17 -2.1
2,322,539 - RALBFv3_RS10435 0.17 +1.1
2,322,673 + RALBFv3_RS10435 0.32 -0.2
2,322,673 + RALBFv3_RS10435 0.32 -0.2
2,322,674 - RALBFv3_RS10435 0.32 +1.7
2,322,823 - RALBFv3_RS10435 0.48 +0.7
2,322,859 + RALBFv3_RS10435 0.52 -0.4
2,323,078 + RALBFv3_RS10435 0.75 -0.3
2,323,079 - RALBFv3_RS10435 0.75 -0.3
2,323,586 + RALBFv3_RS10440 0.29 +0.2
2,323,587 - RALBFv3_RS10440 0.30 -0.3
2,323,587 - RALBFv3_RS10440 0.30 +1.7
2,323,797 + RALBFv3_RS10440 0.52 +0.0
2,323,798 - RALBFv3_RS10440 0.52 +0.4
2,323,899 - RALBFv3_RS10440 0.63 +0.7
2,323,944 - RALBFv3_RS10440 0.67 -1.3
2,323,944 - RALBFv3_RS10440 0.67 +1.7
2,324,129 + RALBFv3_RS10440 0.87 +0.6
2,324,129 + RALBFv3_RS10440 0.87 -0.2
2,324,234 + -0.5
2,324,235 - -0.3
2,324,235 - -0.4
2,324,235 - -0.9
2,324,290 + RALBFv3_RS10445 0.15 -0.2
2,324,525 + +0.5
2,324,526 - -0.1
2,324,604 + -0.3
2,324,604 + +0.4
2,324,605 - -0.3
2,324,605 - -0.8
2,324,634 - RALBFv3_RS10450 0.11 -0.6
2,324,667 - RALBFv3_RS10450 0.22 +1.7
2,324,694 + RALBFv3_RS10450 0.32 -1.0
2,324,695 - RALBFv3_RS10450 0.32 -0.8
2,324,695 - RALBFv3_RS10450 0.32 -0.7
2,324,695 - RALBFv3_RS10450 0.32 -0.2
2,324,811 + RALBFv3_RS10450 0.73 +0.9
2,324,812 - RALBFv3_RS10450 0.73 -0.3
2,324,852 + RALBFv3_RS10450 0.87 +1.7
2,324,852 + RALBFv3_RS10450 0.87 +0.3
2,324,852 + RALBFv3_RS10450 0.87 +0.1
2,324,852 + RALBFv3_RS10450 0.87 +1.7
2,324,853 - RALBFv3_RS10450 0.88 +0.7
2,324,853 - RALBFv3_RS10450 0.88 -0.2
2,324,876 + +0.5
2,324,877 - +0.0
2,325,076 - RALBFv3_RS10455 0.45 +0.1
2,325,174 + RALBFv3_RS10455 0.69 -2.9
2,325,185 + RALBFv3_RS10455 0.71 +0.5
2,325,185 + RALBFv3_RS10455 0.71 +1.1
2,325,186 - RALBFv3_RS10455 0.71 -0.2
2,325,186 - RALBFv3_RS10455 0.71 +0.1
2,325,186 - RALBFv3_RS10455 0.71 -0.3
2,325,188 + RALBFv3_RS10455 0.72 -0.5
2,325,188 + RALBFv3_RS10455 0.72 -1.0
2,325,188 + RALBFv3_RS10455 0.72 +0.1
2,325,189 - RALBFv3_RS10455 0.72 -0.0
2,325,189 - RALBFv3_RS10455 0.72 -0.2
2,325,189 - RALBFv3_RS10455 0.72 +0.1
2,325,189 - RALBFv3_RS10455 0.72 +0.4

Or see this region's nucleotide sequence