Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10430 and RALBFv3_RS10435 are separated by 19 nucleotides RALBFv3_RS10435 and RALBFv3_RS10440 are separated by 1 nucleotides RALBFv3_RS10440 and RALBFv3_RS10445 overlap by 2 nucleotides RALBFv3_RS10445 and RALBFv3_RS10450 are separated by 78 nucleotides RALBFv3_RS10450 and RALBFv3_RS10455 overlap by 4 nucleotides
RALBFv3_RS10430: RALBFv3_RS10430 - YbaK/EbsC family protein, at 2,321,855 to 2,322,358
_RS10430
RALBFv3_RS10435: RALBFv3_RS10435 - hydroxymethylglutaryl-CoA lyase, at 2,322,378 to 2,323,307
_RS10435
RALBFv3_RS10440: RALBFv3_RS10440 - glyoxylate/hydroxypyruvate reductase A, at 2,323,309 to 2,324,250
_RS10440
RALBFv3_RS10445: RALBFv3_RS10445 - hypothetical protein, at 2,324,249 to 2,324,524
_RS10445
RALBFv3_RS10450: RALBFv3_RS10450 - hypothetical protein, at 2,324,603 to 2,324,887
_RS10450
RALBFv3_RS10455: RALBFv3_RS10455 - DUF2778 domain-containing protein, at 2,324,884 to 2,325,306
_RS10455
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2322.383 kb on + strand at 2322.384 kb on - strand at 2322.384 kb on - strand at 2322.538 kb on + strand, within RALBFv3_RS10435 at 2322.539 kb on - strand, within RALBFv3_RS10435 at 2322.539 kb on - strand, within RALBFv3_RS10435 at 2322.673 kb on + strand, within RALBFv3_RS10435 at 2322.673 kb on + strand, within RALBFv3_RS10435 at 2322.674 kb on - strand, within RALBFv3_RS10435 at 2322.823 kb on - strand, within RALBFv3_RS10435 at 2322.859 kb on + strand, within RALBFv3_RS10435 at 2323.078 kb on + strand, within RALBFv3_RS10435 at 2323.079 kb on - strand, within RALBFv3_RS10435 at 2323.586 kb on + strand, within RALBFv3_RS10440 at 2323.587 kb on - strand, within RALBFv3_RS10440 at 2323.587 kb on - strand, within RALBFv3_RS10440 at 2323.797 kb on + strand, within RALBFv3_RS10440 at 2323.798 kb on - strand, within RALBFv3_RS10440 at 2323.899 kb on - strand, within RALBFv3_RS10440 at 2323.944 kb on - strand, within RALBFv3_RS10440 at 2323.944 kb on - strand, within RALBFv3_RS10440 at 2324.129 kb on + strand, within RALBFv3_RS10440 at 2324.129 kb on + strand, within RALBFv3_RS10440 at 2324.234 kb on + strand at 2324.235 kb on - strand at 2324.235 kb on - strand at 2324.235 kb on - strand at 2324.290 kb on + strand, within RALBFv3_RS10445 at 2324.525 kb on + strand at 2324.526 kb on - strand at 2324.604 kb on + strand at 2324.604 kb on + strand at 2324.605 kb on - strand at 2324.605 kb on - strand at 2324.634 kb on - strand, within RALBFv3_RS10450 at 2324.667 kb on - strand, within RALBFv3_RS10450 at 2324.694 kb on + strand, within RALBFv3_RS10450 at 2324.695 kb on - strand, within RALBFv3_RS10450 at 2324.695 kb on - strand, within RALBFv3_RS10450 at 2324.695 kb on - strand, within RALBFv3_RS10450 at 2324.811 kb on + strand, within RALBFv3_RS10450 at 2324.812 kb on - strand, within RALBFv3_RS10450 at 2324.852 kb on + strand, within RALBFv3_RS10450 at 2324.852 kb on + strand, within RALBFv3_RS10450 at 2324.852 kb on + strand, within RALBFv3_RS10450 at 2324.852 kb on + strand, within RALBFv3_RS10450 at 2324.853 kb on - strand, within RALBFv3_RS10450 at 2324.853 kb on - strand, within RALBFv3_RS10450 at 2324.876 kb on + strand at 2324.877 kb on - strand at 2325.076 kb on - strand, within RALBFv3_RS10455 at 2325.174 kb on + strand, within RALBFv3_RS10455 at 2325.185 kb on + strand, within RALBFv3_RS10455 at 2325.185 kb on + strand, within RALBFv3_RS10455 at 2325.186 kb on - strand, within RALBFv3_RS10455 at 2325.186 kb on - strand, within RALBFv3_RS10455 at 2325.186 kb on - strand, within RALBFv3_RS10455 at 2325.188 kb on + strand, within RALBFv3_RS10455 at 2325.188 kb on + strand, within RALBFv3_RS10455 at 2325.188 kb on + strand, within RALBFv3_RS10455 at 2325.189 kb on - strand, within RALBFv3_RS10455 at 2325.189 kb on - strand, within RALBFv3_RS10455 at 2325.189 kb on - strand, within RALBFv3_RS10455 at 2325.189 kb on - strand, within RALBFv3_RS10455
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,322,383 + -0.1 2,322,384 - -0.3 2,322,384 - +0.4 2,322,538 + RALBFv3_RS10435 0.17 -1.1 2,322,539 - RALBFv3_RS10435 0.17 -2.1 2,322,539 - RALBFv3_RS10435 0.17 +1.1 2,322,673 + RALBFv3_RS10435 0.32 -0.2 2,322,673 + RALBFv3_RS10435 0.32 -0.2 2,322,674 - RALBFv3_RS10435 0.32 +1.7 2,322,823 - RALBFv3_RS10435 0.48 +0.7 2,322,859 + RALBFv3_RS10435 0.52 -0.4 2,323,078 + RALBFv3_RS10435 0.75 -0.3 2,323,079 - RALBFv3_RS10435 0.75 -0.3 2,323,586 + RALBFv3_RS10440 0.29 +0.2 2,323,587 - RALBFv3_RS10440 0.30 -0.3 2,323,587 - RALBFv3_RS10440 0.30 +1.7 2,323,797 + RALBFv3_RS10440 0.52 +0.0 2,323,798 - RALBFv3_RS10440 0.52 +0.4 2,323,899 - RALBFv3_RS10440 0.63 +0.7 2,323,944 - RALBFv3_RS10440 0.67 -1.3 2,323,944 - RALBFv3_RS10440 0.67 +1.7 2,324,129 + RALBFv3_RS10440 0.87 +0.6 2,324,129 + RALBFv3_RS10440 0.87 -0.2 2,324,234 + -0.5 2,324,235 - -0.3 2,324,235 - -0.4 2,324,235 - -0.9 2,324,290 + RALBFv3_RS10445 0.15 -0.2 2,324,525 + +0.5 2,324,526 - -0.1 2,324,604 + -0.3 2,324,604 + +0.4 2,324,605 - -0.3 2,324,605 - -0.8 2,324,634 - RALBFv3_RS10450 0.11 -0.6 2,324,667 - RALBFv3_RS10450 0.22 +1.7 2,324,694 + RALBFv3_RS10450 0.32 -1.0 2,324,695 - RALBFv3_RS10450 0.32 -0.8 2,324,695 - RALBFv3_RS10450 0.32 -0.7 2,324,695 - RALBFv3_RS10450 0.32 -0.2 2,324,811 + RALBFv3_RS10450 0.73 +0.9 2,324,812 - RALBFv3_RS10450 0.73 -0.3 2,324,852 + RALBFv3_RS10450 0.87 +1.7 2,324,852 + RALBFv3_RS10450 0.87 +0.3 2,324,852 + RALBFv3_RS10450 0.87 +0.1 2,324,852 + RALBFv3_RS10450 0.87 +1.7 2,324,853 - RALBFv3_RS10450 0.88 +0.7 2,324,853 - RALBFv3_RS10450 0.88 -0.2 2,324,876 + +0.5 2,324,877 - +0.0 2,325,076 - RALBFv3_RS10455 0.45 +0.1 2,325,174 + RALBFv3_RS10455 0.69 -2.9 2,325,185 + RALBFv3_RS10455 0.71 +0.5 2,325,185 + RALBFv3_RS10455 0.71 +1.1 2,325,186 - RALBFv3_RS10455 0.71 -0.2 2,325,186 - RALBFv3_RS10455 0.71 +0.1 2,325,186 - RALBFv3_RS10455 0.71 -0.3 2,325,188 + RALBFv3_RS10455 0.72 -0.5 2,325,188 + RALBFv3_RS10455 0.72 -1.0 2,325,188 + RALBFv3_RS10455 0.72 +0.1 2,325,189 - RALBFv3_RS10455 0.72 -0.0 2,325,189 - RALBFv3_RS10455 0.72 -0.2 2,325,189 - RALBFv3_RS10455 0.72 +0.1 2,325,189 - RALBFv3_RS10455 0.72 +0.4
Or see this region's nucleotide sequence