Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS00230

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS00225 and RALBFv3_RS00230 are separated by 79 nucleotidesRALBFv3_RS00230 and RALBFv3_RS00235 are separated by 26 nucleotides RALBFv3_RS00225: RALBFv3_RS00225 - glutathione ABC transporter ATP-binding protein, at 48,259 to 50,382 _RS00225 RALBFv3_RS00230: RALBFv3_RS00230 - MurR/RpiR family transcriptional regulator, at 50,462 to 51,469 _RS00230 RALBFv3_RS00235: RALBFv3_RS00235 - isoaspartyl peptidase/L-asparaginase, at 51,496 to 52,458 _RS00235 Position (kb) 50 51 52Strain fitness (log2 ratio) -2 -1 0 1at 49.532 kb on + strand, within RALBFv3_RS00225at 49.533 kb on - strand, within RALBFv3_RS00225at 49.533 kb on - strand, within RALBFv3_RS00225at 49.976 kb on + strand, within RALBFv3_RS00225at 50.166 kb on + strand, within RALBFv3_RS00225at 50.166 kb on + strand, within RALBFv3_RS00225at 50.201 kb on - strandat 50.201 kb on - strandat 50.264 kb on + strandat 50.429 kb on + strandat 50.593 kb on - strand, within RALBFv3_RS00230at 51.495 kb on + strandat 51.496 kb on - strandat 51.733 kb on + strand, within RALBFv3_RS00235at 51.734 kb on - strand, within RALBFv3_RS00235at 51.925 kb on + strand, within RALBFv3_RS00235at 51.928 kb on + strand, within RALBFv3_RS00235at 51.928 kb on + strand, within RALBFv3_RS00235at 51.929 kb on - strand, within RALBFv3_RS00235at 52.178 kb on - strand, within RALBFv3_RS00235at 52.246 kb on + strand, within RALBFv3_RS00235

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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49,532 + RALBFv3_RS00225 0.60 +0.7
49,533 - RALBFv3_RS00225 0.60 +0.5
49,533 - RALBFv3_RS00225 0.60 +0.5
49,976 + RALBFv3_RS00225 0.81 +0.3
50,166 + RALBFv3_RS00225 0.90 -0.0
50,166 + RALBFv3_RS00225 0.90 +1.1
50,201 - +0.0
50,201 - -1.5
50,264 + +0.3
50,429 + +0.7
50,593 - RALBFv3_RS00230 0.13 -1.2
51,495 + +0.1
51,496 - -0.1
51,733 + RALBFv3_RS00235 0.25 -2.1
51,734 - RALBFv3_RS00235 0.25 +0.4
51,925 + RALBFv3_RS00235 0.45 -0.3
51,928 + RALBFv3_RS00235 0.45 -0.4
51,928 + RALBFv3_RS00235 0.45 -1.7
51,929 - RALBFv3_RS00235 0.45 -0.1
52,178 - RALBFv3_RS00235 0.71 +1.5
52,246 + RALBFv3_RS00235 0.78 -1.1

Or see this region's nucleotide sequence