Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS14085 and RALBFv3_RS14090 are separated by 15 nucleotides RALBFv3_RS14090 and RALBFv3_RS14095 are separated by 49 nucleotides RALBFv3_RS14095 and RALBFv3_RS14100 are separated by 143 nucleotides RALBFv3_RS14100 and RALBFv3_RS14105 are separated by 54 nucleotides
RALBFv3_RS14085: RALBFv3_RS14085 - phytanoyl-CoA dioxygenase, at 3,082,643 to 3,083,251
_RS14085
RALBFv3_RS14090: RALBFv3_RS14090 - glutathione S-transferase, at 3,083,267 to 3,083,908
_RS14090
RALBFv3_RS14095: RALBFv3_RS14095 - PLP-dependent aminotransferase family protein, at 3,083,958 to 3,085,148
_RS14095
RALBFv3_RS14100: RALBFv3_RS14100 - RidA family protein, at 3,085,292 to 3,085,762
_RS14100
RALBFv3_RS14105: RALBFv3_RS14105 - EamA/RhaT family transporter, at 3,085,817 to 3,086,758
_RS14105
Position (kb)
3083
3084
3085
3086 Strain fitness (log2 ratio)
-1
0
1 at 3083.436 kb on + strand, within RALBFv3_RS14090 at 3083.437 kb on - strand, within RALBFv3_RS14090 at 3083.437 kb on - strand, within RALBFv3_RS14090 at 3083.641 kb on - strand, within RALBFv3_RS14090 at 3083.673 kb on + strand, within RALBFv3_RS14090 at 3083.959 kb on + strand at 3084.095 kb on - strand, within RALBFv3_RS14095 at 3084.475 kb on + strand, within RALBFv3_RS14095 at 3084.476 kb on - strand, within RALBFv3_RS14095 at 3084.529 kb on + strand, within RALBFv3_RS14095 at 3084.530 kb on - strand, within RALBFv3_RS14095 at 3084.658 kb on + strand, within RALBFv3_RS14095 at 3084.658 kb on + strand, within RALBFv3_RS14095 at 3084.659 kb on - strand, within RALBFv3_RS14095 at 3084.659 kb on - strand, within RALBFv3_RS14095 at 3084.739 kb on + strand, within RALBFv3_RS14095 at 3084.781 kb on + strand, within RALBFv3_RS14095 at 3084.781 kb on + strand, within RALBFv3_RS14095 at 3084.781 kb on + strand, within RALBFv3_RS14095 at 3084.946 kb on + strand, within RALBFv3_RS14095 at 3085.151 kb on + strand at 3085.151 kb on + strand at 3085.151 kb on + strand at 3085.955 kb on + strand, within RALBFv3_RS14105 at 3085.956 kb on - strand, within RALBFv3_RS14105 at 3086.040 kb on + strand, within RALBFv3_RS14105 at 3086.040 kb on + strand, within RALBFv3_RS14105 at 3086.106 kb on - strand, within RALBFv3_RS14105
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 3,083,436 + RALBFv3_RS14090 0.26 -0.1 3,083,437 - RALBFv3_RS14090 0.26 +0.3 3,083,437 - RALBFv3_RS14090 0.26 -0.2 3,083,641 - RALBFv3_RS14090 0.58 +0.4 3,083,673 + RALBFv3_RS14090 0.63 -0.4 3,083,959 + -0.4 3,084,095 - RALBFv3_RS14095 0.12 +0.3 3,084,475 + RALBFv3_RS14095 0.43 -0.2 3,084,476 - RALBFv3_RS14095 0.43 -1.0 3,084,529 + RALBFv3_RS14095 0.48 -1.0 3,084,530 - RALBFv3_RS14095 0.48 -1.4 3,084,658 + RALBFv3_RS14095 0.59 +0.2 3,084,658 + RALBFv3_RS14095 0.59 -0.2 3,084,659 - RALBFv3_RS14095 0.59 -0.1 3,084,659 - RALBFv3_RS14095 0.59 -0.2 3,084,739 + RALBFv3_RS14095 0.66 -1.4 3,084,781 + RALBFv3_RS14095 0.69 -0.5 3,084,781 + RALBFv3_RS14095 0.69 -0.6 3,084,781 + RALBFv3_RS14095 0.69 +0.0 3,084,946 + RALBFv3_RS14095 0.83 -1.1 3,085,151 + +0.9 3,085,151 + +0.4 3,085,151 + +0.4 3,085,955 + RALBFv3_RS14105 0.15 -0.1 3,085,956 - RALBFv3_RS14105 0.15 -1.6 3,086,040 + RALBFv3_RS14105 0.24 +0.8 3,086,040 + RALBFv3_RS14105 0.24 +0.6 3,086,106 - RALBFv3_RS14105 0.31 -0.3
Or see this region's nucleotide sequence