Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS09740

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS09735 and RALBFv3_RS09740 are separated by 455 nucleotidesRALBFv3_RS09740 and RALBFv3_RS09745 are separated by 95 nucleotides RALBFv3_RS09735: RALBFv3_RS09735 - AMP-dependent synthetase, at 2,166,646 to 2,168,337 _RS09735 RALBFv3_RS09740: RALBFv3_RS09740 - LysR family transcriptional regulator, at 2,168,793 to 2,169,731 _RS09740 RALBFv3_RS09745: RALBFv3_RS09745 - DNA topoisomerase III, at 2,169,827 to 2,172,469 _RS09745 Position (kb) 2168 2169 2170Strain fitness (log2 ratio) -3 -2 -1 0 1at 2167.907 kb on + strand, within RALBFv3_RS09735at 2167.908 kb on - strand, within RALBFv3_RS09735at 2168.306 kb on + strandat 2168.450 kb on + strandat 2168.451 kb on - strandat 2168.510 kb on - strandat 2168.658 kb on - strandat 2168.985 kb on + strand, within RALBFv3_RS09740at 2168.986 kb on - strand, within RALBFv3_RS09740at 2169.307 kb on - strand, within RALBFv3_RS09740at 2169.522 kb on + strand, within RALBFv3_RS09740at 2169.522 kb on + strand, within RALBFv3_RS09740at 2169.522 kb on + strand, within RALBFv3_RS09740at 2169.522 kb on + strand, within RALBFv3_RS09740at 2169.523 kb on - strand, within RALBFv3_RS09740at 2169.523 kb on - strand, within RALBFv3_RS09740at 2169.577 kb on - strand, within RALBFv3_RS09740at 2169.577 kb on - strand, within RALBFv3_RS09740at 2169.609 kb on + strand, within RALBFv3_RS09740at 2169.610 kb on - strand, within RALBFv3_RS09740at 2169.687 kb on + strandat 2169.688 kb on - strandat 2169.689 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,167,907 + RALBFv3_RS09735 0.75 +0.4
2,167,908 - RALBFv3_RS09735 0.75 +1.1
2,168,306 + +0.9
2,168,450 + +1.1
2,168,451 - -0.1
2,168,510 - -1.1
2,168,658 - +0.1
2,168,985 + RALBFv3_RS09740 0.20 +0.1
2,168,986 - RALBFv3_RS09740 0.21 -1.0
2,169,307 - RALBFv3_RS09740 0.55 -0.4
2,169,522 + RALBFv3_RS09740 0.78 -0.8
2,169,522 + RALBFv3_RS09740 0.78 -1.0
2,169,522 + RALBFv3_RS09740 0.78 -1.0
2,169,522 + RALBFv3_RS09740 0.78 +0.2
2,169,523 - RALBFv3_RS09740 0.78 -0.7
2,169,523 - RALBFv3_RS09740 0.78 -0.3
2,169,577 - RALBFv3_RS09740 0.83 -1.1
2,169,577 - RALBFv3_RS09740 0.83 -0.4
2,169,609 + RALBFv3_RS09740 0.87 -3.1
2,169,610 - RALBFv3_RS09740 0.87 +0.1
2,169,687 + -1.0
2,169,688 - -1.4
2,169,689 - -0.1

Or see this region's nucleotide sequence