Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS21725

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS21715 and RALBFv3_RS21720 are separated by 41 nucleotidesRALBFv3_RS21720 and RALBFv3_RS21725 are separated by 13 nucleotidesRALBFv3_RS21725 and RALBFv3_RS21730 are separated by 39 nucleotidesRALBFv3_RS21730 and RALBFv3_RS21735 are separated by 29 nucleotides RALBFv3_RS21715: RALBFv3_RS21715 - flagellar basal-body rod protein FlgG, at 1,598,975 to 1,599,757 _RS21715 RALBFv3_RS21720: RALBFv3_RS21720 - flagellar basal-body rod protein FlgF, at 1,599,799 to 1,600,536 _RS21720 RALBFv3_RS21725: RALBFv3_RS21725 - flagellar hook protein FlgE, at 1,600,550 to 1,601,755 _RS21725 RALBFv3_RS21730: RALBFv3_RS21730 - flagellar basal body rod modification protein FlgD, at 1,601,795 to 1,602,451 _RS21730 RALBFv3_RS21735: RALBFv3_RS21735 - flagellar basal body rod protein FlgC, at 1,602,481 to 1,602,888 _RS21735 Position (kb) 1600 1601 1602Strain fitness (log2 ratio) -2 -1 0 1 2at 1600.241 kb on + strand, within RALBFv3_RS21720at 1600.242 kb on - strand, within RALBFv3_RS21720at 1600.411 kb on - strand, within RALBFv3_RS21720at 1600.520 kb on + strandat 1600.521 kb on - strandat 1600.521 kb on - strandat 1600.521 kb on - strandat 1600.548 kb on - strandat 1600.548 kb on - strandat 1600.887 kb on + strand, within RALBFv3_RS21725at 1600.888 kb on - strand, within RALBFv3_RS21725at 1601.110 kb on - strand, within RALBFv3_RS21725at 1601.110 kb on - strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.139 kb on + strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.140 kb on - strand, within RALBFv3_RS21725at 1601.142 kb on + strand, within RALBFv3_RS21725at 1601.142 kb on + strand, within RALBFv3_RS21725at 1601.142 kb on + strand, within RALBFv3_RS21725at 1601.142 kb on + strand, within RALBFv3_RS21725at 1601.142 kb on + strand, within RALBFv3_RS21725at 1601.142 kb on + strand, within RALBFv3_RS21725at 1601.142 kb on + strand, within RALBFv3_RS21725at 1601.143 kb on - strand, within RALBFv3_RS21725at 1601.143 kb on - strand, within RALBFv3_RS21725at 1601.143 kb on - strand, within RALBFv3_RS21725at 1601.143 kb on - strand, within RALBFv3_RS21725at 1601.143 kb on - strand, within RALBFv3_RS21725at 1601.143 kb on - strand, within RALBFv3_RS21725at 1601.178 kb on + strand, within RALBFv3_RS21725at 1601.178 kb on + strand, within RALBFv3_RS21725at 1601.179 kb on - strand, within RALBFv3_RS21725at 1601.205 kb on + strand, within RALBFv3_RS21725at 1601.205 kb on + strand, within RALBFv3_RS21725at 1601.205 kb on + strand, within RALBFv3_RS21725at 1601.205 kb on + strand, within RALBFv3_RS21725at 1601.206 kb on - strand, within RALBFv3_RS21725at 1601.206 kb on - strand, within RALBFv3_RS21725at 1601.206 kb on - strand, within RALBFv3_RS21725at 1601.206 kb on - strand, within RALBFv3_RS21725at 1601.206 kb on - strand, within RALBFv3_RS21725at 1601.206 kb on - strand, within RALBFv3_RS21725at 1601.210 kb on - strand, within RALBFv3_RS21725at 1601.285 kb on - strand, within RALBFv3_RS21725at 1601.412 kb on + strand, within RALBFv3_RS21725at 1601.413 kb on - strand, within RALBFv3_RS21725at 1601.413 kb on - strand, within RALBFv3_RS21725at 1601.413 kb on - strand, within RALBFv3_RS21725at 1601.451 kb on + strand, within RALBFv3_RS21725at 1601.451 kb on + strand, within RALBFv3_RS21725at 1601.452 kb on - strand, within RALBFv3_RS21725at 1601.487 kb on + strand, within RALBFv3_RS21725at 1601.488 kb on - strand, within RALBFv3_RS21725at 1601.628 kb on + strand, within RALBFv3_RS21725at 1601.628 kb on + strand, within RALBFv3_RS21725at 1601.628 kb on + strand, within RALBFv3_RS21725at 1601.628 kb on + strand, within RALBFv3_RS21725at 1601.628 kb on + strand, within RALBFv3_RS21725at 1601.629 kb on - strand, within RALBFv3_RS21725at 1601.629 kb on - strand, within RALBFv3_RS21725at 1601.629 kb on - strand, within RALBFv3_RS21725at 1601.629 kb on - strand, within RALBFv3_RS21725at 1601.661 kb on + strandat 1601.661 kb on + strandat 1601.796 kb on + strandat 1601.955 kb on + strand, within RALBFv3_RS21730at 1601.955 kb on + strand, within RALBFv3_RS21730at 1601.956 kb on - strand, within RALBFv3_RS21730at 1601.956 kb on - strand, within RALBFv3_RS21730at 1602.006 kb on + strand, within RALBFv3_RS21730at 1602.007 kb on - strand, within RALBFv3_RS21730at 1602.007 kb on - strand, within RALBFv3_RS21730at 1602.046 kb on + strand, within RALBFv3_RS21730at 1602.391 kb on + strandat 1602.551 kb on + strand, within RALBFv3_RS21735at 1602.551 kb on + strand, within RALBFv3_RS21735

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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1,600,241 + RALBFv3_RS21720 0.60 +0.1
1,600,242 - RALBFv3_RS21720 0.60 -0.3
1,600,411 - RALBFv3_RS21720 0.83 +0.1
1,600,520 + +0.1
1,600,521 - +0.3
1,600,521 - -0.1
1,600,521 - +0.4
1,600,548 - +0.1
1,600,548 - +0.4
1,600,887 + RALBFv3_RS21725 0.28 +0.4
1,600,888 - RALBFv3_RS21725 0.28 -0.4
1,601,110 - RALBFv3_RS21725 0.46 +2.0
1,601,110 - RALBFv3_RS21725 0.46 -1.2
1,601,139 + RALBFv3_RS21725 0.49 +0.7
1,601,139 + RALBFv3_RS21725 0.49 -0.1
1,601,139 + RALBFv3_RS21725 0.49 +0.3
1,601,139 + RALBFv3_RS21725 0.49 -0.4
1,601,139 + RALBFv3_RS21725 0.49 +0.3
1,601,139 + RALBFv3_RS21725 0.49 -0.0
1,601,139 + RALBFv3_RS21725 0.49 -0.3
1,601,139 + RALBFv3_RS21725 0.49 +0.4
1,601,139 + RALBFv3_RS21725 0.49 -0.2
1,601,140 - RALBFv3_RS21725 0.49 +0.3
1,601,140 - RALBFv3_RS21725 0.49 +0.4
1,601,140 - RALBFv3_RS21725 0.49 +0.1
1,601,140 - RALBFv3_RS21725 0.49 -0.1
1,601,140 - RALBFv3_RS21725 0.49 +0.1
1,601,140 - RALBFv3_RS21725 0.49 -0.2
1,601,140 - RALBFv3_RS21725 0.49 +0.7
1,601,140 - RALBFv3_RS21725 0.49 +0.4
1,601,142 + RALBFv3_RS21725 0.49 +0.2
1,601,142 + RALBFv3_RS21725 0.49 +0.0
1,601,142 + RALBFv3_RS21725 0.49 +0.2
1,601,142 + RALBFv3_RS21725 0.49 -0.4
1,601,142 + RALBFv3_RS21725 0.49 -0.3
1,601,142 + RALBFv3_RS21725 0.49 +0.5
1,601,142 + RALBFv3_RS21725 0.49 +1.1
1,601,143 - RALBFv3_RS21725 0.49 +0.4
1,601,143 - RALBFv3_RS21725 0.49 -0.5
1,601,143 - RALBFv3_RS21725 0.49 -0.4
1,601,143 - RALBFv3_RS21725 0.49 +0.4
1,601,143 - RALBFv3_RS21725 0.49 +0.4
1,601,143 - RALBFv3_RS21725 0.49 -0.0
1,601,178 + RALBFv3_RS21725 0.52 +0.4
1,601,178 + RALBFv3_RS21725 0.52 +0.7
1,601,179 - RALBFv3_RS21725 0.52 +0.5
1,601,205 + RALBFv3_RS21725 0.54 -0.4
1,601,205 + RALBFv3_RS21725 0.54 +0.6
1,601,205 + RALBFv3_RS21725 0.54 +0.1
1,601,205 + RALBFv3_RS21725 0.54 +0.2
1,601,206 - RALBFv3_RS21725 0.54 +0.8
1,601,206 - RALBFv3_RS21725 0.54 -0.2
1,601,206 - RALBFv3_RS21725 0.54 +0.0
1,601,206 - RALBFv3_RS21725 0.54 -1.9
1,601,206 - RALBFv3_RS21725 0.54 +0.4
1,601,206 - RALBFv3_RS21725 0.54 +1.1
1,601,210 - RALBFv3_RS21725 0.55 -0.1
1,601,285 - RALBFv3_RS21725 0.61 -0.0
1,601,412 + RALBFv3_RS21725 0.71 +0.2
1,601,413 - RALBFv3_RS21725 0.72 -0.1
1,601,413 - RALBFv3_RS21725 0.72 -0.3
1,601,413 - RALBFv3_RS21725 0.72 +0.2
1,601,451 + RALBFv3_RS21725 0.75 -0.4
1,601,451 + RALBFv3_RS21725 0.75 +0.7
1,601,452 - RALBFv3_RS21725 0.75 +0.5
1,601,487 + RALBFv3_RS21725 0.78 +0.1
1,601,488 - RALBFv3_RS21725 0.78 -0.0
1,601,628 + RALBFv3_RS21725 0.89 +0.4
1,601,628 + RALBFv3_RS21725 0.89 +1.0
1,601,628 + RALBFv3_RS21725 0.89 -0.1
1,601,628 + RALBFv3_RS21725 0.89 +0.3
1,601,628 + RALBFv3_RS21725 0.89 +0.2
1,601,629 - RALBFv3_RS21725 0.89 +0.1
1,601,629 - RALBFv3_RS21725 0.89 +0.9
1,601,629 - RALBFv3_RS21725 0.89 -0.1
1,601,629 - RALBFv3_RS21725 0.89 +0.7
1,601,661 + -0.9
1,601,661 + +0.4
1,601,796 + +0.7
1,601,955 + RALBFv3_RS21730 0.24 +0.2
1,601,955 + RALBFv3_RS21730 0.24 +0.4
1,601,956 - RALBFv3_RS21730 0.25 +0.1
1,601,956 - RALBFv3_RS21730 0.25 +0.9
1,602,006 + RALBFv3_RS21730 0.32 +0.0
1,602,007 - RALBFv3_RS21730 0.32 +0.2
1,602,007 - RALBFv3_RS21730 0.32 +0.8
1,602,046 + RALBFv3_RS21730 0.38 +0.4
1,602,391 + +0.1
1,602,551 + RALBFv3_RS21735 0.17 +0.5
1,602,551 + RALBFv3_RS21735 0.17 +0.0

Or see this region's nucleotide sequence