Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS18115 and RALBFv3_RS18120 overlap by 4 nucleotides RALBFv3_RS18120 and RALBFv3_RS18125 are separated by 58 nucleotides
RALBFv3_RS18115: RALBFv3_RS18115 - nodulation protein, at 586,925 to 590,026
_RS18115
RALBFv3_RS18120: RALBFv3_RS18120 - multidrug transporter subunit MdtB, at 590,023 to 593,169
_RS18120
RALBFv3_RS18125: RALBFv3_RS18125 - multidrug transporter subunit MdtA, at 593,228 to 594,565
_RS18125
Position (kb) 590
591
592
593
594 Strain fitness (log2 ratio) -2
-1
0
1 at 589.111 kb on - strand, within RALBFv3_RS18115 at 589.213 kb on - strand, within RALBFv3_RS18115 at 589.306 kb on - strand, within RALBFv3_RS18115 at 589.306 kb on - strand, within RALBFv3_RS18115 at 589.306 kb on - strand, within RALBFv3_RS18115 at 589.339 kb on - strand, within RALBFv3_RS18115 at 589.437 kb on + strand, within RALBFv3_RS18115 at 589.437 kb on + strand, within RALBFv3_RS18115 at 589.437 kb on + strand, within RALBFv3_RS18115 at 589.438 kb on - strand, within RALBFv3_RS18115 at 589.438 kb on - strand, within RALBFv3_RS18115 at 589.438 kb on - strand, within RALBFv3_RS18115 at 589.438 kb on - strand, within RALBFv3_RS18115 at 590.141 kb on + strand at 590.142 kb on - strand at 590.142 kb on - strand at 590.142 kb on - strand at 590.142 kb on - strand at 590.142 kb on - strand at 590.324 kb on + strand at 590.325 kb on - strand at 590.543 kb on + strand, within RALBFv3_RS18120 at 590.544 kb on - strand, within RALBFv3_RS18120 at 590.564 kb on + strand, within RALBFv3_RS18120 at 590.564 kb on + strand, within RALBFv3_RS18120 at 590.564 kb on + strand, within RALBFv3_RS18120 at 590.564 kb on + strand, within RALBFv3_RS18120 at 590.565 kb on - strand, within RALBFv3_RS18120 at 590.565 kb on - strand, within RALBFv3_RS18120 at 590.565 kb on - strand, within RALBFv3_RS18120 at 590.565 kb on - strand, within RALBFv3_RS18120 at 590.565 kb on - strand, within RALBFv3_RS18120 at 590.568 kb on + strand, within RALBFv3_RS18120 at 590.568 kb on + strand, within RALBFv3_RS18120 at 590.569 kb on - strand, within RALBFv3_RS18120 at 590.864 kb on + strand, within RALBFv3_RS18120 at 590.919 kb on - strand, within RALBFv3_RS18120 at 590.919 kb on - strand, within RALBFv3_RS18120 at 590.972 kb on + strand, within RALBFv3_RS18120 at 591.008 kb on + strand, within RALBFv3_RS18120 at 591.041 kb on + strand, within RALBFv3_RS18120 at 591.041 kb on + strand, within RALBFv3_RS18120 at 591.042 kb on - strand, within RALBFv3_RS18120 at 591.173 kb on + strand, within RALBFv3_RS18120 at 591.528 kb on + strand, within RALBFv3_RS18120 at 591.530 kb on + strand, within RALBFv3_RS18120 at 591.530 kb on + strand, within RALBFv3_RS18120 at 591.531 kb on - strand, within RALBFv3_RS18120 at 591.531 kb on - strand, within RALBFv3_RS18120 at 591.531 kb on - strand, within RALBFv3_RS18120 at 591.531 kb on - strand, within RALBFv3_RS18120 at 591.537 kb on - strand, within RALBFv3_RS18120 at 591.537 kb on - strand, within RALBFv3_RS18120 at 591.665 kb on + strand, within RALBFv3_RS18120 at 591.666 kb on - strand, within RALBFv3_RS18120 at 591.666 kb on - strand, within RALBFv3_RS18120 at 592.038 kb on - strand, within RALBFv3_RS18120 at 592.038 kb on - strand, within RALBFv3_RS18120 at 592.428 kb on - strand, within RALBFv3_RS18120 at 592.448 kb on + strand, within RALBFv3_RS18120 at 592.784 kb on + strand, within RALBFv3_RS18120 at 592.785 kb on - strand, within RALBFv3_RS18120 at 592.785 kb on - strand, within RALBFv3_RS18120 at 592.785 kb on - strand, within RALBFv3_RS18120 at 593.045 kb on + strand at 593.802 kb on + strand, within RALBFv3_RS18125 at 593.802 kb on + strand, within RALBFv3_RS18125 at 593.802 kb on + strand, within RALBFv3_RS18125 at 593.803 kb on - strand, within RALBFv3_RS18125 at 593.803 kb on - strand, within RALBFv3_RS18125 at 593.803 kb on - strand, within RALBFv3_RS18125 at 593.803 kb on - strand, within RALBFv3_RS18125 at 593.929 kb on + strand, within RALBFv3_RS18125 at 593.931 kb on + strand, within RALBFv3_RS18125 at 594.064 kb on - strand, within RALBFv3_RS18125
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 589,111 - RALBFv3_RS18115 0.70 +0.1 589,213 - RALBFv3_RS18115 0.74 -0.1 589,306 - RALBFv3_RS18115 0.77 -0.1 589,306 - RALBFv3_RS18115 0.77 -0.1 589,306 - RALBFv3_RS18115 0.77 -0.1 589,339 - RALBFv3_RS18115 0.78 -0.2 589,437 + RALBFv3_RS18115 0.81 -0.4 589,437 + RALBFv3_RS18115 0.81 +1.5 589,437 + RALBFv3_RS18115 0.81 -0.5 589,438 - RALBFv3_RS18115 0.81 -0.3 589,438 - RALBFv3_RS18115 0.81 -0.0 589,438 - RALBFv3_RS18115 0.81 -0.2 589,438 - RALBFv3_RS18115 0.81 -0.1 590,141 + -0.1 590,142 - -0.1 590,142 - +0.1 590,142 - -0.4 590,142 - +0.2 590,142 - -0.1 590,324 + -0.3 590,325 - -0.1 590,543 + RALBFv3_RS18120 0.17 +0.3 590,544 - RALBFv3_RS18120 0.17 -0.4 590,564 + RALBFv3_RS18120 0.17 -0.6 590,564 + RALBFv3_RS18120 0.17 +0.1 590,564 + RALBFv3_RS18120 0.17 +0.1 590,564 + RALBFv3_RS18120 0.17 -0.4 590,565 - RALBFv3_RS18120 0.17 -0.5 590,565 - RALBFv3_RS18120 0.17 +0.4 590,565 - RALBFv3_RS18120 0.17 -0.1 590,565 - RALBFv3_RS18120 0.17 +0.1 590,565 - RALBFv3_RS18120 0.17 -0.6 590,568 + RALBFv3_RS18120 0.17 +0.0 590,568 + RALBFv3_RS18120 0.17 -0.4 590,569 - RALBFv3_RS18120 0.17 -0.1 590,864 + RALBFv3_RS18120 0.27 -0.9 590,919 - RALBFv3_RS18120 0.28 -0.4 590,919 - RALBFv3_RS18120 0.28 -0.3 590,972 + RALBFv3_RS18120 0.30 +0.2 591,008 + RALBFv3_RS18120 0.31 -0.3 591,041 + RALBFv3_RS18120 0.32 +0.1 591,041 + RALBFv3_RS18120 0.32 -0.7 591,042 - RALBFv3_RS18120 0.32 +1.3 591,173 + RALBFv3_RS18120 0.37 -0.5 591,528 + RALBFv3_RS18120 0.48 -0.3 591,530 + RALBFv3_RS18120 0.48 -0.2 591,530 + RALBFv3_RS18120 0.48 -0.3 591,531 - RALBFv3_RS18120 0.48 -0.2 591,531 - RALBFv3_RS18120 0.48 -0.5 591,531 - RALBFv3_RS18120 0.48 -0.7 591,531 - RALBFv3_RS18120 0.48 -0.1 591,537 - RALBFv3_RS18120 0.48 +0.2 591,537 - RALBFv3_RS18120 0.48 -1.2 591,665 + RALBFv3_RS18120 0.52 +0.0 591,666 - RALBFv3_RS18120 0.52 -0.1 591,666 - RALBFv3_RS18120 0.52 -1.6 592,038 - RALBFv3_RS18120 0.64 -0.5 592,038 - RALBFv3_RS18120 0.64 -0.2 592,428 - RALBFv3_RS18120 0.76 -0.3 592,448 + RALBFv3_RS18120 0.77 -0.1 592,784 + RALBFv3_RS18120 0.88 +0.4 592,785 - RALBFv3_RS18120 0.88 -0.1 592,785 - RALBFv3_RS18120 0.88 -0.4 592,785 - RALBFv3_RS18120 0.88 +0.1 593,045 + -0.1 593,802 + RALBFv3_RS18125 0.43 -0.9 593,802 + RALBFv3_RS18125 0.43 +0.0 593,802 + RALBFv3_RS18125 0.43 -0.1 593,803 - RALBFv3_RS18125 0.43 +0.3 593,803 - RALBFv3_RS18125 0.43 -1.1 593,803 - RALBFv3_RS18125 0.43 +0.1 593,803 - RALBFv3_RS18125 0.43 -0.3 593,929 + RALBFv3_RS18125 0.52 -1.9 593,931 + RALBFv3_RS18125 0.53 -0.5 594,064 - RALBFv3_RS18125 0.62 -0.2
Or see this region's nucleotide sequence