Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS11150

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS11145 and RALBFv3_RS11150 are separated by 93 nucleotidesRALBFv3_RS11150 and RALBFv3_RS11155 are separated by 190 nucleotides RALBFv3_RS11145: RALBFv3_RS11145 - RNase adapter RapZ, at 2,459,600 to 2,460,490 _RS11145 RALBFv3_RS11150: RALBFv3_RS11150 - hypothetical protein, at 2,460,584 to 2,460,916 _RS11150 RALBFv3_RS11155: RALBFv3_RS11155 - HPr kinase/phosphorylase, at 2,461,107 to 2,462,081 _RS11155 Position (kb) 2460 2461Strain fitness (log2 ratio) -2 -1 0 1at 2459.602 kb on - strandat 2459.610 kb on - strandat 2459.641 kb on + strandat 2459.658 kb on + strandat 2459.683 kb on - strandat 2459.683 kb on - strandat 2459.730 kb on + strand, within RALBFv3_RS11145at 2459.775 kb on + strand, within RALBFv3_RS11145at 2459.775 kb on + strand, within RALBFv3_RS11145at 2459.776 kb on - strand, within RALBFv3_RS11145at 2459.878 kb on - strand, within RALBFv3_RS11145at 2460.006 kb on + strand, within RALBFv3_RS11145at 2460.006 kb on + strand, within RALBFv3_RS11145at 2460.007 kb on - strand, within RALBFv3_RS11145at 2460.290 kb on + strand, within RALBFv3_RS11145at 2460.346 kb on - strand, within RALBFv3_RS11145at 2460.363 kb on + strand, within RALBFv3_RS11145at 2460.412 kb on - strandat 2460.501 kb on + strandat 2460.745 kb on - strand, within RALBFv3_RS11150

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,459,602 - +0.9
2,459,610 - +0.1
2,459,641 + +0.9
2,459,658 + +0.9
2,459,683 - -1.5
2,459,683 - +0.2
2,459,730 + RALBFv3_RS11145 0.15 -0.7
2,459,775 + RALBFv3_RS11145 0.20 -0.1
2,459,775 + RALBFv3_RS11145 0.20 -1.5
2,459,776 - RALBFv3_RS11145 0.20 -2.1
2,459,878 - RALBFv3_RS11145 0.31 -0.1
2,460,006 + RALBFv3_RS11145 0.46 -2.5
2,460,006 + RALBFv3_RS11145 0.46 -0.1
2,460,007 - RALBFv3_RS11145 0.46 -1.1
2,460,290 + RALBFv3_RS11145 0.77 -1.5
2,460,346 - RALBFv3_RS11145 0.84 -1.8
2,460,363 + RALBFv3_RS11145 0.86 -1.5
2,460,412 - +0.3
2,460,501 + +0.4
2,460,745 - RALBFv3_RS11150 0.48 +0.3

Or see this region's nucleotide sequence