Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03605 and RALBFv3_RS03610 are separated by 34 nucleotides RALBFv3_RS03610 and RALBFv3_RS03615 are separated by 5 nucleotides
RALBFv3_RS03605: RALBFv3_RS03605 - glycosyltransferase family 2 protein, at 790,431 to 791,201
_RS03605
RALBFv3_RS03610: RALBFv3_RS03610 - glycosyl transferase, at 791,236 to 792,156
_RS03610
RALBFv3_RS03615: RALBFv3_RS03615 - ligase, at 792,162 to 793,469
_RS03615
Position (kb) 791
792
793 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 790.249 kb on + strand at 790.343 kb on + strand at 790.344 kb on - strand at 790.821 kb on + strand, within RALBFv3_RS03605 at 790.822 kb on - strand, within RALBFv3_RS03605 at 790.932 kb on + strand, within RALBFv3_RS03605 at 790.933 kb on - strand, within RALBFv3_RS03605 at 790.939 kb on - strand, within RALBFv3_RS03605 at 790.939 kb on - strand, within RALBFv3_RS03605 at 791.336 kb on + strand, within RALBFv3_RS03610 at 791.336 kb on + strand, within RALBFv3_RS03610 at 791.423 kb on + strand, within RALBFv3_RS03610 at 791.424 kb on - strand, within RALBFv3_RS03610 at 791.424 kb on - strand, within RALBFv3_RS03610 at 791.525 kb on + strand, within RALBFv3_RS03610 at 791.631 kb on - strand, within RALBFv3_RS03610 at 791.832 kb on - strand, within RALBFv3_RS03610 at 791.897 kb on + strand, within RALBFv3_RS03610 at 791.897 kb on + strand, within RALBFv3_RS03610 at 791.898 kb on - strand, within RALBFv3_RS03610 at 791.898 kb on - strand at 791.898 kb on - strand, within RALBFv3_RS03610 at 791.994 kb on + strand, within RALBFv3_RS03610 at 791.994 kb on + strand, within RALBFv3_RS03610 at 791.994 kb on + strand, within RALBFv3_RS03610 at 791.995 kb on - strand, within RALBFv3_RS03610 at 792.451 kb on + strand, within RALBFv3_RS03615 at 792.451 kb on + strand, within RALBFv3_RS03615 at 792.452 kb on - strand, within RALBFv3_RS03615 at 792.608 kb on - strand, within RALBFv3_RS03615 at 792.714 kb on - strand, within RALBFv3_RS03615 at 792.716 kb on - strand, within RALBFv3_RS03615
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 790,249 + -2.1 790,343 + -0.4 790,344 - -2.7 790,821 + RALBFv3_RS03605 0.51 -4.1 790,822 - RALBFv3_RS03605 0.51 -2.8 790,932 + RALBFv3_RS03605 0.65 -3.3 790,933 - RALBFv3_RS03605 0.65 -1.2 790,939 - RALBFv3_RS03605 0.66 -3.5 790,939 - RALBFv3_RS03605 0.66 -1.6 791,336 + RALBFv3_RS03610 0.11 -0.2 791,336 + RALBFv3_RS03610 0.11 -3.2 791,423 + RALBFv3_RS03610 0.20 -2.6 791,424 - RALBFv3_RS03610 0.20 -3.0 791,424 - RALBFv3_RS03610 0.20 -2.4 791,525 + RALBFv3_RS03610 0.31 -2.1 791,631 - RALBFv3_RS03610 0.43 -2.8 791,832 - RALBFv3_RS03610 0.65 -2.4 791,897 + RALBFv3_RS03610 0.72 -0.9 791,897 + RALBFv3_RS03610 0.72 -1.6 791,898 - RALBFv3_RS03610 0.72 -1.9 791,898 - -0.2 791,898 - RALBFv3_RS03610 0.72 -1.1 791,994 + RALBFv3_RS03610 0.82 -1.2 791,994 + RALBFv3_RS03610 0.82 -2.6 791,994 + RALBFv3_RS03610 0.82 -3.1 791,995 - RALBFv3_RS03610 0.82 -2.7 792,451 + RALBFv3_RS03615 0.22 +0.6 792,451 + RALBFv3_RS03615 0.22 -0.2 792,452 - RALBFv3_RS03615 0.22 -1.5 792,608 - RALBFv3_RS03615 0.34 -1.6 792,714 - RALBFv3_RS03615 0.42 -1.6 792,716 - RALBFv3_RS03615 0.42 -1.9
Or see this region's nucleotide sequence