Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS18145 and RALBFv3_RS18150 are separated by 186 nucleotides RALBFv3_RS18150 and RALBFv3_RS18155 are separated by 657 nucleotides
RALBFv3_RS18145: RALBFv3_RS18145 - phospholipid carrier-dependent glycosyltransferase, at 597,464 to 599,119
_RS18145
RALBFv3_RS18150: RALBFv3_RS18150 - DNA-binding response regulator, at 599,306 to 599,575
_RS18150
RALBFv3_RS18155: RALBFv3_RS18155 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 600,233 to 600,967
_RS18155
Position (kb) 599
600 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 598.344 kb on + strand, within RALBFv3_RS18145 at 598.344 kb on + strand, within RALBFv3_RS18145 at 598.345 kb on - strand, within RALBFv3_RS18145 at 598.345 kb on - strand, within RALBFv3_RS18145 at 598.542 kb on + strand, within RALBFv3_RS18145 at 598.542 kb on + strand, within RALBFv3_RS18145 at 598.753 kb on - strand, within RALBFv3_RS18145 at 598.809 kb on + strand, within RALBFv3_RS18145 at 598.951 kb on - strand, within RALBFv3_RS18145 at 599.197 kb on + strand at 599.206 kb on - strand at 599.307 kb on + strand at 599.308 kb on - strand at 599.308 kb on - strand at 599.343 kb on + strand, within RALBFv3_RS18150 at 599.343 kb on - strand, within RALBFv3_RS18150 at 599.344 kb on - strand, within RALBFv3_RS18150 at 599.344 kb on - strand, within RALBFv3_RS18150 at 599.591 kb on + strand at 599.592 kb on - strand at 599.592 kb on - strand at 599.592 kb on - strand at 599.635 kb on + strand at 599.635 kb on + strand at 599.635 kb on + strand at 599.649 kb on + strand at 599.649 kb on + strand at 599.650 kb on - strand at 599.657 kb on + strand at 599.918 kb on - strand at 599.953 kb on - strand at 600.259 kb on - strand at 600.259 kb on - strand at 600.469 kb on + strand, within RALBFv3_RS18155 at 600.470 kb on - strand, within RALBFv3_RS18155
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 598,344 + RALBFv3_RS18145 0.53 -7.0 598,344 + RALBFv3_RS18145 0.53 -6.1 598,345 - RALBFv3_RS18145 0.53 -7.3 598,345 - RALBFv3_RS18145 0.53 -6.3 598,542 + RALBFv3_RS18145 0.65 +0.8 598,542 + RALBFv3_RS18145 0.65 -5.8 598,753 - RALBFv3_RS18145 0.78 -5.6 598,809 + RALBFv3_RS18145 0.81 -3.0 598,951 - RALBFv3_RS18145 0.90 -6.3 599,197 + -4.6 599,206 - -0.4 599,307 + -1.4 599,308 - -0.4 599,308 - -1.4 599,343 + RALBFv3_RS18150 0.14 -0.4 599,343 - RALBFv3_RS18150 0.14 -0.0 599,344 - RALBFv3_RS18150 0.14 -0.9 599,344 - RALBFv3_RS18150 0.14 +0.3 599,591 + -0.1 599,592 - +0.3 599,592 - -0.2 599,592 - -0.5 599,635 + -0.0 599,635 + -0.2 599,635 + -0.4 599,649 + -0.2 599,649 + -0.2 599,650 - +0.5 599,657 + -0.7 599,918 - -1.7 599,953 - -0.3 600,259 - +0.8 600,259 - -0.1 600,469 + RALBFv3_RS18155 0.32 +1.4 600,470 - RALBFv3_RS18155 0.32 +0.0
Or see this region's nucleotide sequence