Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12540

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12535 and RALBFv3_RS12540 are separated by 128 nucleotidesRALBFv3_RS12540 and RALBFv3_RS12545 are separated by 532 nucleotides RALBFv3_RS12535: RALBFv3_RS12535 - precorrin-2 dehydrogenase, at 2,760,966 to 2,761,499 _RS12535 RALBFv3_RS12540: RALBFv3_RS12540 - Flp family type IVb pilin, at 2,761,628 to 2,761,804 _RS12540 RALBFv3_RS12545: RALBFv3_RS12545 - Flp family type IVb pilin, at 2,762,337 to 2,762,522 _RS12545 Position (kb) 2761 2762Strain fitness (log2 ratio) -2 -1 0 1 2at 2760.852 kb on - strandat 2760.852 kb on - strandat 2760.960 kb on - strandat 2760.967 kb on + strandat 2760.967 kb on + strandat 2760.967 kb on + strandat 2760.967 kb on + strandat 2760.968 kb on - strandat 2760.968 kb on - strandat 2761.168 kb on - strand, within RALBFv3_RS12535at 2761.278 kb on + strand, within RALBFv3_RS12535at 2761.279 kb on - strand, within RALBFv3_RS12535at 2761.279 kb on - strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.282 kb on + strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.283 kb on - strand, within RALBFv3_RS12535at 2761.705 kb on - strand, within RALBFv3_RS12540at 2761.746 kb on + strand, within RALBFv3_RS12540at 2761.746 kb on + strand, within RALBFv3_RS12540at 2761.746 kb on + strand, within RALBFv3_RS12540at 2761.746 kb on + strand, within RALBFv3_RS12540at 2761.747 kb on - strand, within RALBFv3_RS12540at 2762.338 kb on + strandat 2762.338 kb on + strandat 2762.364 kb on + strand, within RALBFv3_RS12545at 2762.365 kb on - strand, within RALBFv3_RS12545at 2762.365 kb on - strand, within RALBFv3_RS12545at 2762.464 kb on + strand, within RALBFv3_RS12545at 2762.465 kb on - strand, within RALBFv3_RS12545

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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2,760,852 - -1.2
2,760,852 - -0.1
2,760,960 - -1.2
2,760,967 + +0.2
2,760,967 + +0.3
2,760,967 + -0.2
2,760,967 + -0.7
2,760,968 - -1.1
2,760,968 - +1.1
2,761,168 - RALBFv3_RS12535 0.38 +2.1
2,761,278 + RALBFv3_RS12535 0.58 -0.2
2,761,279 - RALBFv3_RS12535 0.59 -0.8
2,761,279 - RALBFv3_RS12535 0.59 -1.6
2,761,282 + RALBFv3_RS12535 0.59 -0.1
2,761,282 + RALBFv3_RS12535 0.59 -0.0
2,761,282 + RALBFv3_RS12535 0.59 -0.0
2,761,282 + RALBFv3_RS12535 0.59 -0.7
2,761,282 + RALBFv3_RS12535 0.59 +0.2
2,761,282 + RALBFv3_RS12535 0.59 -0.0
2,761,282 + RALBFv3_RS12535 0.59 -0.3
2,761,282 + RALBFv3_RS12535 0.59 +0.2
2,761,282 + RALBFv3_RS12535 0.59 +0.1
2,761,282 + RALBFv3_RS12535 0.59 +1.1
2,761,282 + RALBFv3_RS12535 0.59 -0.1
2,761,282 + RALBFv3_RS12535 0.59 +0.8
2,761,282 + RALBFv3_RS12535 0.59 -0.9
2,761,282 + RALBFv3_RS12535 0.59 -0.1
2,761,282 + RALBFv3_RS12535 0.59 +1.1
2,761,282 + RALBFv3_RS12535 0.59 -0.2
2,761,283 - RALBFv3_RS12535 0.59 -0.5
2,761,283 - RALBFv3_RS12535 0.59 -0.1
2,761,283 - RALBFv3_RS12535 0.59 -0.1
2,761,283 - RALBFv3_RS12535 0.59 +1.1
2,761,283 - RALBFv3_RS12535 0.59 -2.2
2,761,283 - RALBFv3_RS12535 0.59 +1.7
2,761,283 - RALBFv3_RS12535 0.59 +0.1
2,761,283 - RALBFv3_RS12535 0.59 +1.1
2,761,705 - RALBFv3_RS12540 0.44 -0.0
2,761,746 + RALBFv3_RS12540 0.67 -0.0
2,761,746 + RALBFv3_RS12540 0.67 -1.7
2,761,746 + RALBFv3_RS12540 0.67 +1.1
2,761,746 + RALBFv3_RS12540 0.67 +0.2
2,761,747 - RALBFv3_RS12540 0.67 -1.4
2,762,338 + +0.4
2,762,338 + -0.1
2,762,364 + RALBFv3_RS12545 0.15 -0.5
2,762,365 - RALBFv3_RS12545 0.15 -0.1
2,762,365 - RALBFv3_RS12545 0.15 +1.1
2,762,464 + RALBFv3_RS12545 0.68 +0.0
2,762,465 - RALBFv3_RS12545 0.69 +0.2

Or see this region's nucleotide sequence