Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12535 and RALBFv3_RS12540 are separated by 128 nucleotides RALBFv3_RS12540 and RALBFv3_RS12545 are separated by 532 nucleotides
RALBFv3_RS12535: RALBFv3_RS12535 - precorrin-2 dehydrogenase, at 2,760,966 to 2,761,499
_RS12535
RALBFv3_RS12540: RALBFv3_RS12540 - Flp family type IVb pilin, at 2,761,628 to 2,761,804
_RS12540
RALBFv3_RS12545: RALBFv3_RS12545 - Flp family type IVb pilin, at 2,762,337 to 2,762,522
_RS12545
Position (kb)
2761
2762 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2760.852 kb on - strand at 2760.852 kb on - strand at 2760.960 kb on - strand at 2760.967 kb on + strand at 2760.967 kb on + strand at 2760.967 kb on + strand at 2760.967 kb on + strand at 2760.968 kb on - strand at 2760.968 kb on - strand at 2761.168 kb on - strand, within RALBFv3_RS12535 at 2761.278 kb on + strand, within RALBFv3_RS12535 at 2761.279 kb on - strand, within RALBFv3_RS12535 at 2761.279 kb on - strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.282 kb on + strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.283 kb on - strand, within RALBFv3_RS12535 at 2761.705 kb on - strand, within RALBFv3_RS12540 at 2761.746 kb on + strand, within RALBFv3_RS12540 at 2761.746 kb on + strand, within RALBFv3_RS12540 at 2761.746 kb on + strand, within RALBFv3_RS12540 at 2761.746 kb on + strand, within RALBFv3_RS12540 at 2761.747 kb on - strand, within RALBFv3_RS12540 at 2762.338 kb on + strand at 2762.338 kb on + strand at 2762.364 kb on + strand, within RALBFv3_RS12545 at 2762.365 kb on - strand, within RALBFv3_RS12545 at 2762.365 kb on - strand, within RALBFv3_RS12545 at 2762.464 kb on + strand, within RALBFv3_RS12545 at 2762.465 kb on - strand, within RALBFv3_RS12545
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 2,760,852 - -1.2 2,760,852 - -0.1 2,760,960 - -1.2 2,760,967 + +0.2 2,760,967 + +0.3 2,760,967 + -0.2 2,760,967 + -0.7 2,760,968 - -1.1 2,760,968 - +1.1 2,761,168 - RALBFv3_RS12535 0.38 +2.1 2,761,278 + RALBFv3_RS12535 0.58 -0.2 2,761,279 - RALBFv3_RS12535 0.59 -0.8 2,761,279 - RALBFv3_RS12535 0.59 -1.6 2,761,282 + RALBFv3_RS12535 0.59 -0.1 2,761,282 + RALBFv3_RS12535 0.59 -0.0 2,761,282 + RALBFv3_RS12535 0.59 -0.0 2,761,282 + RALBFv3_RS12535 0.59 -0.7 2,761,282 + RALBFv3_RS12535 0.59 +0.2 2,761,282 + RALBFv3_RS12535 0.59 -0.0 2,761,282 + RALBFv3_RS12535 0.59 -0.3 2,761,282 + RALBFv3_RS12535 0.59 +0.2 2,761,282 + RALBFv3_RS12535 0.59 +0.1 2,761,282 + RALBFv3_RS12535 0.59 +1.1 2,761,282 + RALBFv3_RS12535 0.59 -0.1 2,761,282 + RALBFv3_RS12535 0.59 +0.8 2,761,282 + RALBFv3_RS12535 0.59 -0.9 2,761,282 + RALBFv3_RS12535 0.59 -0.1 2,761,282 + RALBFv3_RS12535 0.59 +1.1 2,761,282 + RALBFv3_RS12535 0.59 -0.2 2,761,283 - RALBFv3_RS12535 0.59 -0.5 2,761,283 - RALBFv3_RS12535 0.59 -0.1 2,761,283 - RALBFv3_RS12535 0.59 -0.1 2,761,283 - RALBFv3_RS12535 0.59 +1.1 2,761,283 - RALBFv3_RS12535 0.59 -2.2 2,761,283 - RALBFv3_RS12535 0.59 +1.7 2,761,283 - RALBFv3_RS12535 0.59 +0.1 2,761,283 - RALBFv3_RS12535 0.59 +1.1 2,761,705 - RALBFv3_RS12540 0.44 -0.0 2,761,746 + RALBFv3_RS12540 0.67 -0.0 2,761,746 + RALBFv3_RS12540 0.67 -1.7 2,761,746 + RALBFv3_RS12540 0.67 +1.1 2,761,746 + RALBFv3_RS12540 0.67 +0.2 2,761,747 - RALBFv3_RS12540 0.67 -1.4 2,762,338 + +0.4 2,762,338 + -0.1 2,762,364 + RALBFv3_RS12545 0.15 -0.5 2,762,365 - RALBFv3_RS12545 0.15 -0.1 2,762,365 - RALBFv3_RS12545 0.15 +1.1 2,762,464 + RALBFv3_RS12545 0.68 +0.0 2,762,465 - RALBFv3_RS12545 0.69 +0.2
Or see this region's nucleotide sequence