Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10175

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10170 and RALBFv3_RS10175 are separated by 170 nucleotidesRALBFv3_RS10175 and RALBFv3_RS10180 overlap by 4 nucleotides RALBFv3_RS10170: RALBFv3_RS10170 - ornithine--oxo-acid transaminase, at 2,260,293 to 2,261,519 _RS10170 RALBFv3_RS10175: RALBFv3_RS10175 - LysR family transcriptional regulator, at 2,261,690 to 2,262,619 _RS10175 RALBFv3_RS10180: RALBFv3_RS10180 - aldehyde dehydrogenase family protein, at 2,262,616 to 2,264,127 _RS10180 Position (kb) 2261 2262 2263Strain fitness (log2 ratio) -1 0 1 2at 2260.891 kb on + strand, within RALBFv3_RS10170at 2261.368 kb on + strand, within RALBFv3_RS10170at 2261.458 kb on + strandat 2261.458 kb on + strandat 2261.459 kb on - strandat 2261.459 kb on - strandat 2261.712 kb on - strandat 2261.719 kb on + strandat 2261.719 kb on + strandat 2261.720 kb on - strandat 2261.720 kb on - strandat 2262.005 kb on + strand, within RALBFv3_RS10175at 2262.005 kb on + strand, within RALBFv3_RS10175at 2262.005 kb on + strand, within RALBFv3_RS10175at 2262.192 kb on - strand, within RALBFv3_RS10175at 2262.294 kb on - strand, within RALBFv3_RS10175at 2262.314 kb on + strand, within RALBFv3_RS10175at 2262.314 kb on + strand, within RALBFv3_RS10175at 2262.315 kb on - strand, within RALBFv3_RS10175at 2262.315 kb on - strand, within RALBFv3_RS10175at 2262.679 kb on - strandat 2262.698 kb on + strandat 2262.698 kb on + strandat 2262.699 kb on - strandat 2263.019 kb on + strand, within RALBFv3_RS10180at 2263.022 kb on + strand, within RALBFv3_RS10180at 2263.022 kb on + strand, within RALBFv3_RS10180at 2263.118 kb on + strand, within RALBFv3_RS10180at 2263.191 kb on - strand, within RALBFv3_RS10180at 2263.224 kb on - strand, within RALBFv3_RS10180

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
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2,260,891 + RALBFv3_RS10170 0.49 -1.0
2,261,368 + RALBFv3_RS10170 0.88 -0.3
2,261,458 + +0.3
2,261,458 + -0.4
2,261,459 - +0.7
2,261,459 - +0.1
2,261,712 - +0.5
2,261,719 + -0.6
2,261,719 + +0.3
2,261,720 - +1.2
2,261,720 - +0.1
2,262,005 + RALBFv3_RS10175 0.34 -1.4
2,262,005 + RALBFv3_RS10175 0.34 -0.1
2,262,005 + RALBFv3_RS10175 0.34 -0.6
2,262,192 - RALBFv3_RS10175 0.54 -1.0
2,262,294 - RALBFv3_RS10175 0.65 -0.1
2,262,314 + RALBFv3_RS10175 0.67 -0.8
2,262,314 + RALBFv3_RS10175 0.67 -0.7
2,262,315 - RALBFv3_RS10175 0.67 -1.3
2,262,315 - RALBFv3_RS10175 0.67 +0.2
2,262,679 - +0.3
2,262,698 + -0.1
2,262,698 + -0.8
2,262,699 - +1.8
2,263,019 + RALBFv3_RS10180 0.27 -0.1
2,263,022 + RALBFv3_RS10180 0.27 -0.6
2,263,022 + RALBFv3_RS10180 0.27 -0.3
2,263,118 + RALBFv3_RS10180 0.33 +1.2
2,263,191 - RALBFv3_RS10180 0.38 +0.3
2,263,224 - RALBFv3_RS10180 0.40 -0.4

Or see this region's nucleotide sequence