Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS21460 and RALBFv3_RS21465 are separated by 224 nucleotides RALBFv3_RS21465 and RALBFv3_RS24640 are separated by 29 nucleotides RALBFv3_RS24640 and RALBFv3_RS21470 are separated by 71 nucleotides
RALBFv3_RS21460: RALBFv3_RS21460 - TonB-dependent siderophore receptor, at 1,537,650 to 1,540,076
_RS21460
RALBFv3_RS21465: RALBFv3_RS21465 - RNA polymerase sigma factor, at 1,540,301 to 1,540,891
_RS21465
RALBFv3_RS24640: RALBFv3_RS24640 - hypothetical protein, at 1,540,921 to 1,541,100
_RS24640
RALBFv3_RS21470: RALBFv3_RS21470 - TonB-dependent siderophore receptor, at 1,541,172 to 1,543,409
_RS21470
Position (kb) 1540
1541 Strain fitness (log2 ratio) -2
-1
0
1 at 1539.430 kb on - strand, within RALBFv3_RS21460 at 1540.005 kb on - strand at 1540.116 kb on - strand at 1540.156 kb on + strand at 1540.156 kb on + strand at 1540.156 kb on + strand at 1540.158 kb on - strand at 1540.204 kb on + strand at 1540.332 kb on + strand at 1540.429 kb on + strand, within RALBFv3_RS21465 at 1540.430 kb on - strand, within RALBFv3_RS21465 at 1540.825 kb on - strand, within RALBFv3_RS21465 at 1541.505 kb on + strand, within RALBFv3_RS21470 at 1541.505 kb on + strand, within RALBFv3_RS21470 at 1541.506 kb on - strand, within RALBFv3_RS21470 at 1541.506 kb on - strand, within RALBFv3_RS21470 at 1541.616 kb on - strand, within RALBFv3_RS21470 at 1541.617 kb on - strand, within RALBFv3_RS21470 at 1541.617 kb on - strand, within RALBFv3_RS21470 at 1541.617 kb on - strand, within RALBFv3_RS21470 at 1541.617 kb on - strand, within RALBFv3_RS21470 at 1541.617 kb on - strand, within RALBFv3_RS21470 at 1541.617 kb on - strand, within RALBFv3_RS21470 at 1541.617 kb on - strand, within RALBFv3_RS21470 at 1541.676 kb on + strand, within RALBFv3_RS21470 at 1541.677 kb on - strand, within RALBFv3_RS21470 at 1541.677 kb on - strand, within RALBFv3_RS21470 at 1541.677 kb on - strand, within RALBFv3_RS21470 at 1541.677 kb on - strand, within RALBFv3_RS21470 at 1541.694 kb on + strand, within RALBFv3_RS21470 at 1541.694 kb on + strand, within RALBFv3_RS21470 at 1541.694 kb on + strand, within RALBFv3_RS21470 at 1541.694 kb on + strand, within RALBFv3_RS21470 at 1541.695 kb on - strand, within RALBFv3_RS21470 at 1541.695 kb on - strand, within RALBFv3_RS21470 at 1541.712 kb on + strand, within RALBFv3_RS21470 at 1541.713 kb on - strand, within RALBFv3_RS21470 at 1541.713 kb on - strand, within RALBFv3_RS21470 at 1541.758 kb on - strand, within RALBFv3_RS21470 at 1541.850 kb on + strand, within RALBFv3_RS21470 at 1541.850 kb on + strand, within RALBFv3_RS21470 at 1541.851 kb on - strand, within RALBFv3_RS21470 at 1541.851 kb on - strand, within RALBFv3_RS21470
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,539,430 - RALBFv3_RS21460 0.73 -1.2 1,540,005 - -1.3 1,540,116 - -2.6 1,540,156 + -0.3 1,540,156 + -0.6 1,540,156 + -1.6 1,540,158 - -0.2 1,540,204 + -0.5 1,540,332 + -0.6 1,540,429 + RALBFv3_RS21465 0.22 -1.3 1,540,430 - RALBFv3_RS21465 0.22 -1.3 1,540,825 - RALBFv3_RS21465 0.89 +0.2 1,541,505 + RALBFv3_RS21470 0.15 -1.0 1,541,505 + RALBFv3_RS21470 0.15 -0.0 1,541,506 - RALBFv3_RS21470 0.15 +0.7 1,541,506 - RALBFv3_RS21470 0.15 -0.1 1,541,616 - RALBFv3_RS21470 0.20 +0.4 1,541,617 - RALBFv3_RS21470 0.20 +0.1 1,541,617 - RALBFv3_RS21470 0.20 -0.4 1,541,617 - RALBFv3_RS21470 0.20 +0.0 1,541,617 - RALBFv3_RS21470 0.20 +0.2 1,541,617 - RALBFv3_RS21470 0.20 +0.3 1,541,617 - RALBFv3_RS21470 0.20 +0.1 1,541,617 - RALBFv3_RS21470 0.20 +0.8 1,541,676 + RALBFv3_RS21470 0.23 -1.1 1,541,677 - RALBFv3_RS21470 0.23 -0.6 1,541,677 - RALBFv3_RS21470 0.23 -0.1 1,541,677 - RALBFv3_RS21470 0.23 +0.4 1,541,677 - RALBFv3_RS21470 0.23 +0.7 1,541,694 + RALBFv3_RS21470 0.23 -0.8 1,541,694 + RALBFv3_RS21470 0.23 +0.4 1,541,694 + RALBFv3_RS21470 0.23 -0.5 1,541,694 + RALBFv3_RS21470 0.23 -0.4 1,541,695 - RALBFv3_RS21470 0.23 -0.1 1,541,695 - RALBFv3_RS21470 0.23 +0.4 1,541,712 + RALBFv3_RS21470 0.24 +0.5 1,541,713 - RALBFv3_RS21470 0.24 -0.0 1,541,713 - RALBFv3_RS21470 0.24 +0.3 1,541,758 - RALBFv3_RS21470 0.26 -0.1 1,541,850 + RALBFv3_RS21470 0.30 +0.1 1,541,850 + RALBFv3_RS21470 0.30 +0.2 1,541,851 - RALBFv3_RS21470 0.30 -0.3 1,541,851 - RALBFv3_RS21470 0.30 -1.4
Or see this region's nucleotide sequence