Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS20275 and RALBFv3_RS20280 are separated by 45 nucleotides RALBFv3_RS20280 and RALBFv3_RS20285 are separated by 100 nucleotides RALBFv3_RS20285 and RALBFv3_RS20290 overlap by 4 nucleotides
RALBFv3_RS20275: RALBFv3_RS20275 - EamA/RhaT family transporter, at 1,241,436 to 1,242,332
_RS20275
RALBFv3_RS20280: RALBFv3_RS20280 - tRNA synthetase subunit beta, at 1,242,378 to 1,243,094
_RS20280
RALBFv3_RS20285: RALBFv3_RS20285 - YggS family pyridoxal phosphate-dependent enzyme, at 1,243,195 to 1,243,875
_RS20285
RALBFv3_RS20290: RALBFv3_RS20290 - stachydrine utilization oxidoreductase protein, at 1,243,872 to 1,246,073
_RS20290
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-1
0
1 at 1241.395 kb on - strand at 1241.681 kb on + strand, within RALBFv3_RS20275 at 1241.681 kb on + strand, within RALBFv3_RS20275 at 1241.709 kb on + strand, within RALBFv3_RS20275 at 1241.710 kb on - strand, within RALBFv3_RS20275 at 1241.956 kb on + strand, within RALBFv3_RS20275 at 1242.123 kb on + strand, within RALBFv3_RS20275 at 1242.354 kb on + strand at 1242.354 kb on + strand at 1242.377 kb on + strand at 1242.393 kb on + strand at 1242.394 kb on - strand at 1242.394 kb on - strand at 1242.394 kb on - strand at 1242.394 kb on - strand at 1242.394 kb on - strand at 1242.394 kb on - strand at 1242.394 kb on - strand at 1242.399 kb on + strand at 1242.400 kb on - strand at 1242.415 kb on + strand at 1242.457 kb on + strand, within RALBFv3_RS20280 at 1242.457 kb on + strand, within RALBFv3_RS20280 at 1242.457 kb on + strand, within RALBFv3_RS20280 at 1242.490 kb on + strand, within RALBFv3_RS20280 at 1242.490 kb on + strand, within RALBFv3_RS20280 at 1242.612 kb on + strand, within RALBFv3_RS20280 at 1242.795 kb on + strand, within RALBFv3_RS20280 at 1242.913 kb on + strand, within RALBFv3_RS20280 at 1242.913 kb on + strand, within RALBFv3_RS20280 at 1243.194 kb on + strand at 1243.194 kb on + strand at 1243.194 kb on + strand at 1243.195 kb on - strand at 1243.355 kb on + strand, within RALBFv3_RS20285 at 1243.570 kb on + strand, within RALBFv3_RS20285 at 1243.571 kb on - strand, within RALBFv3_RS20285 at 1243.571 kb on - strand, within RALBFv3_RS20285 at 1243.661 kb on - strand, within RALBFv3_RS20285 at 1243.661 kb on - strand, within RALBFv3_RS20285 at 1243.792 kb on + strand, within RALBFv3_RS20285 at 1243.793 kb on - strand, within RALBFv3_RS20285 at 1243.793 kb on - strand, within RALBFv3_RS20285 at 1243.793 kb on - strand, within RALBFv3_RS20285 at 1243.793 kb on - strand, within RALBFv3_RS20285 at 1243.968 kb on + strand at 1243.969 kb on - strand at 1243.969 kb on - strand at 1244.088 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,241,395 - -0.6 1,241,681 + RALBFv3_RS20275 0.27 +0.7 1,241,681 + RALBFv3_RS20275 0.27 +0.1 1,241,709 + RALBFv3_RS20275 0.30 -0.2 1,241,710 - RALBFv3_RS20275 0.31 +0.2 1,241,956 + RALBFv3_RS20275 0.58 -1.3 1,242,123 + RALBFv3_RS20275 0.77 -0.5 1,242,354 + +0.5 1,242,354 + -0.0 1,242,377 + -0.0 1,242,393 + -0.7 1,242,394 - -0.3 1,242,394 - +1.5 1,242,394 - -0.6 1,242,394 - -0.3 1,242,394 - -0.7 1,242,394 - -0.4 1,242,394 - -0.3 1,242,399 + +0.2 1,242,400 - +0.7 1,242,415 + -0.7 1,242,457 + RALBFv3_RS20280 0.11 -0.6 1,242,457 + RALBFv3_RS20280 0.11 -0.1 1,242,457 + RALBFv3_RS20280 0.11 +0.1 1,242,490 + RALBFv3_RS20280 0.16 +0.2 1,242,490 + RALBFv3_RS20280 0.16 -0.6 1,242,612 + RALBFv3_RS20280 0.33 -0.0 1,242,795 + RALBFv3_RS20280 0.58 -0.2 1,242,913 + RALBFv3_RS20280 0.75 -0.9 1,242,913 + RALBFv3_RS20280 0.75 +0.7 1,243,194 + -0.5 1,243,194 + +0.7 1,243,194 + +0.0 1,243,195 - +0.6 1,243,355 + RALBFv3_RS20285 0.23 +0.0 1,243,570 + RALBFv3_RS20285 0.55 -0.3 1,243,571 - RALBFv3_RS20285 0.55 +0.2 1,243,571 - RALBFv3_RS20285 0.55 -0.3 1,243,661 - RALBFv3_RS20285 0.68 -0.0 1,243,661 - RALBFv3_RS20285 0.68 +0.1 1,243,792 + RALBFv3_RS20285 0.88 +0.5 1,243,793 - RALBFv3_RS20285 0.88 +0.3 1,243,793 - RALBFv3_RS20285 0.88 -0.9 1,243,793 - RALBFv3_RS20285 0.88 -0.4 1,243,793 - RALBFv3_RS20285 0.88 +0.2 1,243,968 + -0.5 1,243,969 - +0.5 1,243,969 - -1.7 1,244,088 + +0.2
Or see this region's nucleotide sequence