Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS20280

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS20275 and RALBFv3_RS20280 are separated by 45 nucleotidesRALBFv3_RS20280 and RALBFv3_RS20285 are separated by 100 nucleotidesRALBFv3_RS20285 and RALBFv3_RS20290 overlap by 4 nucleotides RALBFv3_RS20275: RALBFv3_RS20275 - EamA/RhaT family transporter, at 1,241,436 to 1,242,332 _RS20275 RALBFv3_RS20280: RALBFv3_RS20280 - tRNA synthetase subunit beta, at 1,242,378 to 1,243,094 _RS20280 RALBFv3_RS20285: RALBFv3_RS20285 - YggS family pyridoxal phosphate-dependent enzyme, at 1,243,195 to 1,243,875 _RS20285 RALBFv3_RS20290: RALBFv3_RS20290 - stachydrine utilization oxidoreductase protein, at 1,243,872 to 1,246,073 _RS20290 Position (kb) 1242 1243 1244Strain fitness (log2 ratio) -1 0 1at 1241.395 kb on - strandat 1241.681 kb on + strand, within RALBFv3_RS20275at 1241.681 kb on + strand, within RALBFv3_RS20275at 1241.709 kb on + strand, within RALBFv3_RS20275at 1241.710 kb on - strand, within RALBFv3_RS20275at 1241.956 kb on + strand, within RALBFv3_RS20275at 1242.123 kb on + strand, within RALBFv3_RS20275at 1242.354 kb on + strandat 1242.354 kb on + strandat 1242.377 kb on + strandat 1242.393 kb on + strandat 1242.394 kb on - strandat 1242.394 kb on - strandat 1242.394 kb on - strandat 1242.394 kb on - strandat 1242.394 kb on - strandat 1242.394 kb on - strandat 1242.394 kb on - strandat 1242.399 kb on + strandat 1242.400 kb on - strandat 1242.415 kb on + strandat 1242.457 kb on + strand, within RALBFv3_RS20280at 1242.457 kb on + strand, within RALBFv3_RS20280at 1242.457 kb on + strand, within RALBFv3_RS20280at 1242.490 kb on + strand, within RALBFv3_RS20280at 1242.490 kb on + strand, within RALBFv3_RS20280at 1242.612 kb on + strand, within RALBFv3_RS20280at 1242.795 kb on + strand, within RALBFv3_RS20280at 1242.913 kb on + strand, within RALBFv3_RS20280at 1242.913 kb on + strand, within RALBFv3_RS20280at 1243.194 kb on + strandat 1243.194 kb on + strandat 1243.194 kb on + strandat 1243.195 kb on - strandat 1243.355 kb on + strand, within RALBFv3_RS20285at 1243.570 kb on + strand, within RALBFv3_RS20285at 1243.571 kb on - strand, within RALBFv3_RS20285at 1243.571 kb on - strand, within RALBFv3_RS20285at 1243.661 kb on - strand, within RALBFv3_RS20285at 1243.661 kb on - strand, within RALBFv3_RS20285at 1243.792 kb on + strand, within RALBFv3_RS20285at 1243.793 kb on - strand, within RALBFv3_RS20285at 1243.793 kb on - strand, within RALBFv3_RS20285at 1243.793 kb on - strand, within RALBFv3_RS20285at 1243.793 kb on - strand, within RALBFv3_RS20285at 1243.968 kb on + strandat 1243.969 kb on - strandat 1243.969 kb on - strandat 1244.088 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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1,241,395 - -0.6
1,241,681 + RALBFv3_RS20275 0.27 +0.7
1,241,681 + RALBFv3_RS20275 0.27 +0.1
1,241,709 + RALBFv3_RS20275 0.30 -0.2
1,241,710 - RALBFv3_RS20275 0.31 +0.2
1,241,956 + RALBFv3_RS20275 0.58 -1.3
1,242,123 + RALBFv3_RS20275 0.77 -0.5
1,242,354 + +0.5
1,242,354 + -0.0
1,242,377 + -0.0
1,242,393 + -0.7
1,242,394 - -0.3
1,242,394 - +1.5
1,242,394 - -0.6
1,242,394 - -0.3
1,242,394 - -0.7
1,242,394 - -0.4
1,242,394 - -0.3
1,242,399 + +0.2
1,242,400 - +0.7
1,242,415 + -0.7
1,242,457 + RALBFv3_RS20280 0.11 -0.6
1,242,457 + RALBFv3_RS20280 0.11 -0.1
1,242,457 + RALBFv3_RS20280 0.11 +0.1
1,242,490 + RALBFv3_RS20280 0.16 +0.2
1,242,490 + RALBFv3_RS20280 0.16 -0.6
1,242,612 + RALBFv3_RS20280 0.33 -0.0
1,242,795 + RALBFv3_RS20280 0.58 -0.2
1,242,913 + RALBFv3_RS20280 0.75 -0.9
1,242,913 + RALBFv3_RS20280 0.75 +0.7
1,243,194 + -0.5
1,243,194 + +0.7
1,243,194 + +0.0
1,243,195 - +0.6
1,243,355 + RALBFv3_RS20285 0.23 +0.0
1,243,570 + RALBFv3_RS20285 0.55 -0.3
1,243,571 - RALBFv3_RS20285 0.55 +0.2
1,243,571 - RALBFv3_RS20285 0.55 -0.3
1,243,661 - RALBFv3_RS20285 0.68 -0.0
1,243,661 - RALBFv3_RS20285 0.68 +0.1
1,243,792 + RALBFv3_RS20285 0.88 +0.5
1,243,793 - RALBFv3_RS20285 0.88 +0.3
1,243,793 - RALBFv3_RS20285 0.88 -0.9
1,243,793 - RALBFv3_RS20285 0.88 -0.4
1,243,793 - RALBFv3_RS20285 0.88 +0.2
1,243,968 + -0.5
1,243,969 - +0.5
1,243,969 - -1.7
1,244,088 + +0.2

Or see this region's nucleotide sequence