Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03615 and RALBFv3_RS03620 are separated by 114 nucleotides RALBFv3_RS03620 and RALBFv3_RS03625 are separated by 149 nucleotides
RALBFv3_RS03615: RALBFv3_RS03615 - ligase, at 792,162 to 793,469
_RS03615
RALBFv3_RS03620: RALBFv3_RS03620 - DNA polymerase III subunit alpha, at 793,584 to 797,108
_RS03620
RALBFv3_RS03625: RALBFv3_RS03625 - sulfurtransferase, at 797,258 to 798,106
_RS03625
Position (kb) 793
794
795
796
797
798 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 792.608 kb on - strand, within RALBFv3_RS03615 at 792.714 kb on - strand, within RALBFv3_RS03615 at 792.716 kb on - strand, within RALBFv3_RS03615 at 793.393 kb on + strand at 793.393 kb on + strand at 793.423 kb on + strand at 793.554 kb on + strand at 793.554 kb on + strand at 797.282 kb on + strand at 797.666 kb on - strand, within RALBFv3_RS03625 at 797.867 kb on + strand, within RALBFv3_RS03625 at 797.867 kb on + strand, within RALBFv3_RS03625 at 797.868 kb on - strand, within RALBFv3_RS03625 at 797.868 kb on - strand, within RALBFv3_RS03625 at 797.868 kb on - strand, within RALBFv3_RS03625 at 797.868 kb on - strand, within RALBFv3_RS03625 at 797.894 kb on + strand, within RALBFv3_RS03625 at 797.895 kb on - strand, within RALBFv3_RS03625 at 797.921 kb on + strand, within RALBFv3_RS03625 at 797.921 kb on + strand, within RALBFv3_RS03625 at 797.921 kb on + strand, within RALBFv3_RS03625 at 798.047 kb on + strand at 798.047 kb on + strand at 798.048 kb on - strand at 798.048 kb on - strand at 798.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 792,608 - RALBFv3_RS03615 0.34 -0.4 792,714 - RALBFv3_RS03615 0.42 -1.4 792,716 - RALBFv3_RS03615 0.42 -4.1 793,393 + -1.4 793,393 + -3.6 793,423 + -2.2 793,554 + -0.5 793,554 + +0.1 797,282 + -2.4 797,666 - RALBFv3_RS03625 0.48 -0.7 797,867 + RALBFv3_RS03625 0.72 +0.2 797,867 + RALBFv3_RS03625 0.72 -1.0 797,868 - RALBFv3_RS03625 0.72 -0.2 797,868 - RALBFv3_RS03625 0.72 -0.3 797,868 - RALBFv3_RS03625 0.72 +0.6 797,868 - RALBFv3_RS03625 0.72 -0.7 797,894 + RALBFv3_RS03625 0.75 -0.0 797,895 - RALBFv3_RS03625 0.75 -1.7 797,921 + RALBFv3_RS03625 0.78 +0.2 797,921 + RALBFv3_RS03625 0.78 -1.0 797,921 + RALBFv3_RS03625 0.78 +1.1 798,047 + +0.9 798,047 + -0.5 798,048 - -0.5 798,048 - +1.3 798,048 - -0.8
Or see this region's nucleotide sequence