Experiment: R2A control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1544 and SMa1545 overlap by 1 nucleotides SMa1545 and SMa1547 are separated by 294 nucleotides SMa1547 and SMa1548 are separated by 141 nucleotides
SMa1544: SMa1544 - NuoK2 NADH:quinone oxidoreductase subunit 11 (chain K), at 852,312 to 852,614
SMa1544
SMa1545: SMa1545 - NuoJ2 NADH:quinone oxidoreductase subunit 6 (chain J), at 852,614 to 853,114
SMa1545
SMa1547: SMa1547 - protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT), at 853,409 to 854,080
SMa1547
SMa1548: SMa1548 - Diguanylate cyclase/phosphodiesterase, at 854,222 to 857,437
SMa1548
Position (kb) 853
854
855 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 852.457 kb on + strand, within SMa1544 at 852.458 kb on - strand, within SMa1544 at 852.460 kb on + strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.461 kb on - strand, within SMa1544 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.936 kb on + strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.937 kb on - strand, within SMa1545 at 852.980 kb on - strand, within SMa1545 at 853.168 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.194 kb on + strand at 853.217 kb on + strand at 853.217 kb on + strand at 853.277 kb on + strand at 853.278 kb on - strand at 853.278 kb on - strand at 853.337 kb on + strand at 853.338 kb on - strand at 853.338 kb on - strand at 853.359 kb on - strand at 853.476 kb on - strand at 853.624 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.828 kb on + strand, within SMa1547 at 853.829 kb on - strand, within SMa1547 at 853.829 kb on - strand, within SMa1547 at 853.829 kb on - strand, within SMa1547 at 854.078 kb on + strand at 854.078 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.111 kb on + strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.112 kb on - strand at 854.115 kb on + strand at 854.116 kb on - strand at 854.168 kb on + strand at 854.210 kb on - strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.254 kb on + strand at 854.255 kb on - strand at 854.281 kb on + strand at 854.281 kb on + strand at 854.281 kb on + strand at 854.309 kb on - strand at 854.309 kb on - strand at 854.409 kb on + strand at 854.409 kb on + strand at 854.409 kb on + strand at 854.410 kb on - strand at 854.410 kb on - strand at 854.410 kb on - strand at 854.410 kb on - strand at 854.457 kb on + strand at 854.458 kb on - strand at 854.603 kb on - strand, within SMa1548 at 854.654 kb on + strand, within SMa1548 at 854.873 kb on + strand, within SMa1548 at 854.874 kb on - strand, within SMa1548 at 854.874 kb on - strand, within SMa1548 at 854.903 kb on - strand, within SMa1548 at 854.982 kb on + strand, within SMa1548
Per-strain Table
Position Strand Gene LocusTag Fraction R2A control remove 852,457 + SMa1544 0.48 +0.5 852,458 - SMa1544 0.48 +0.3 852,460 + SMa1544 0.49 -0.4 852,461 - SMa1544 0.49 +0.3 852,461 - SMa1544 0.49 +0.3 852,936 + SMa1545 0.64 +1.0 852,936 + SMa1545 0.64 -0.0 852,936 + SMa1545 0.64 -0.9 852,937 - SMa1545 0.64 +0.2 852,937 - SMa1545 0.64 +0.8 852,980 - SMa1545 0.73 +1.1 853,168 + -0.3 853,194 + +1.5 853,194 + +2.7 853,194 + +0.3 853,217 + -0.0 853,217 + +0.2 853,277 + +2.2 853,278 - -0.4 853,278 - +0.5 853,337 + +0.4 853,338 - -0.4 853,338 - -0.1 853,359 - +0.1 853,476 - +0.1 853,624 + SMa1547 0.32 +0.3 853,828 + SMa1547 0.62 -0.7 853,828 + SMa1547 0.62 -2.9 853,828 + SMa1547 0.62 -1.5 853,828 + SMa1547 0.62 -0.0 853,829 - SMa1547 0.62 -1.7 853,829 - SMa1547 0.62 -0.2 853,829 - SMa1547 0.62 -0.6 854,078 + -0.3 854,078 + +0.7 854,111 + +0.4 854,111 + -0.8 854,111 + +0.1 854,111 + -1.1 854,111 + +0.3 854,112 - +0.9 854,112 - +0.7 854,112 - +0.1 854,112 - +0.4 854,112 - +0.5 854,115 + +0.3 854,116 - +0.6 854,168 + +0.7 854,210 - -0.3 854,254 + +1.4 854,254 + -1.2 854,254 + +0.1 854,254 + -0.5 854,254 + -0.5 854,254 + -1.5 854,255 - -0.0 854,281 + +0.1 854,281 + -1.0 854,281 + -0.7 854,309 - +0.4 854,309 - +1.2 854,409 + -0.7 854,409 + +0.1 854,409 + +0.2 854,410 - +0.1 854,410 - +0.6 854,410 - +0.1 854,410 - -0.3 854,457 + +0.1 854,458 - +0.2 854,603 - SMa1548 0.12 -0.4 854,654 + SMa1548 0.13 -0.4 854,873 + SMa1548 0.20 +3.2 854,874 - SMa1548 0.20 +4.2 854,874 - SMa1548 0.20 +2.0 854,903 - SMa1548 0.21 -0.1 854,982 + SMa1548 0.24 +0.1
Or see this region's nucleotide sequence