Experiment: rhizosphere sample 1; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03225 and BWI76_RS03230 are separated by 48 nucleotides BWI76_RS03230 and BWI76_RS03235 overlap by 4 nucleotides
BWI76_RS03225: BWI76_RS03225 - transcriptional regulator, at 664,764 to 665,570
_RS03225
BWI76_RS03230: BWI76_RS03230 - hypothetical protein, at 665,619 to 666,524
_RS03230
BWI76_RS03235: BWI76_RS03235 - Neopullulanase (EC 3.2.1.135) <small>(from data)</small>, at 666,521 to 668,317
_RS03235
Position (kb) 665
666
667 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 664.627 kb on - strand at 664.649 kb on + strand at 664.649 kb on + strand at 664.658 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.660 kb on + strand at 664.666 kb on + strand at 664.668 kb on + strand at 664.668 kb on + strand at 664.668 kb on + strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.668 kb on - strand at 664.671 kb on + strand at 664.679 kb on - strand at 664.679 kb on - strand at 664.681 kb on + strand at 664.690 kb on + strand at 664.696 kb on - strand at 664.700 kb on + strand at 664.719 kb on + strand at 664.738 kb on + strand at 664.738 kb on + strand at 664.748 kb on - strand at 664.748 kb on - strand at 664.793 kb on - strand at 664.795 kb on + strand at 664.798 kb on - strand at 664.820 kb on + strand at 664.836 kb on - strand at 665.006 kb on - strand, within BWI76_RS03225 at 665.091 kb on + strand, within BWI76_RS03225 at 665.100 kb on + strand, within BWI76_RS03225 at 665.100 kb on + strand, within BWI76_RS03225 at 665.108 kb on - strand at 665.108 kb on - strand, within BWI76_RS03225 at 665.130 kb on - strand, within BWI76_RS03225 at 665.130 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.155 kb on - strand, within BWI76_RS03225 at 665.158 kb on + strand, within BWI76_RS03225 at 665.190 kb on - strand, within BWI76_RS03225 at 665.261 kb on - strand, within BWI76_RS03225 at 665.261 kb on - strand, within BWI76_RS03225 at 665.275 kb on + strand, within BWI76_RS03225 at 665.279 kb on - strand, within BWI76_RS03225 at 665.307 kb on - strand, within BWI76_RS03225 at 665.443 kb on - strand, within BWI76_RS03225 at 665.445 kb on - strand, within BWI76_RS03225 at 665.476 kb on - strand, within BWI76_RS03225 at 665.477 kb on - strand, within BWI76_RS03225 at 665.513 kb on - strand at 665.513 kb on - strand at 665.513 kb on - strand at 665.513 kb on - strand at 665.513 kb on - strand at 665.543 kb on - strand at 665.543 kb on - strand at 665.543 kb on - strand at 665.559 kb on - strand at 665.579 kb on + strand at 665.580 kb on - strand at 665.580 kb on - strand at 665.587 kb on - strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.630 kb on + strand at 665.631 kb on + strand at 665.635 kb on + strand at 665.635 kb on + strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.638 kb on - strand at 665.640 kb on - strand at 665.645 kb on - strand at 665.648 kb on - strand at 665.648 kb on - strand at 665.648 kb on - strand at 665.662 kb on + strand at 665.677 kb on - strand at 665.739 kb on + strand, within BWI76_RS03230 at 665.753 kb on - strand, within BWI76_RS03230 at 665.753 kb on - strand, within BWI76_RS03230 at 665.775 kb on + strand, within BWI76_RS03230 at 665.805 kb on - strand, within BWI76_RS03230 at 665.812 kb on - strand, within BWI76_RS03230 at 665.841 kb on + strand, within BWI76_RS03230 at 665.843 kb on + strand, within BWI76_RS03230 at 665.848 kb on + strand, within BWI76_RS03230 at 665.848 kb on + strand, within BWI76_RS03230 at 665.848 kb on + strand, within BWI76_RS03230 at 665.851 kb on - strand, within BWI76_RS03230 at 665.874 kb on - strand, within BWI76_RS03230 at 665.875 kb on + strand, within BWI76_RS03230 at 665.878 kb on - strand, within BWI76_RS03230 at 665.904 kb on + strand, within BWI76_RS03230 at 665.912 kb on - strand, within BWI76_RS03230 at 665.932 kb on + strand, within BWI76_RS03230 at 665.943 kb on - strand, within BWI76_RS03230 at 666.032 kb on + strand, within BWI76_RS03230 at 666.040 kb on - strand, within BWI76_RS03230 at 666.087 kb on - strand, within BWI76_RS03230 at 666.136 kb on + strand, within BWI76_RS03230 at 666.161 kb on + strand, within BWI76_RS03230 at 666.201 kb on - strand, within BWI76_RS03230 at 666.214 kb on - strand, within BWI76_RS03230 at 666.237 kb on + strand, within BWI76_RS03230 at 666.238 kb on - strand, within BWI76_RS03230 at 666.303 kb on + strand, within BWI76_RS03230 at 666.303 kb on + strand, within BWI76_RS03230 at 666.316 kb on + strand, within BWI76_RS03230 at 666.319 kb on - strand, within BWI76_RS03230 at 666.326 kb on - strand, within BWI76_RS03230 at 666.326 kb on - strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.330 kb on + strand, within BWI76_RS03230 at 666.333 kb on - strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.335 kb on + strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.338 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.343 kb on - strand, within BWI76_RS03230 at 666.406 kb on + strand, within BWI76_RS03230 at 666.415 kb on - strand, within BWI76_RS03230 at 666.415 kb on - strand, within BWI76_RS03230 at 666.415 kb on - strand, within BWI76_RS03230 at 666.416 kb on - strand, within BWI76_RS03230 at 666.436 kb on - strand at 666.442 kb on - strand at 666.451 kb on - strand at 666.451 kb on - strand at 666.518 kb on - strand at 666.528 kb on + strand at 666.549 kb on - strand at 666.564 kb on - strand at 666.570 kb on - strand at 666.571 kb on + strand at 666.579 kb on - strand at 666.583 kb on + strand at 666.585 kb on + strand at 666.585 kb on + strand at 666.591 kb on - strand at 666.593 kb on - strand at 666.593 kb on - strand at 666.593 kb on - strand at 666.593 kb on - strand at 666.638 kb on + strand at 666.654 kb on - strand at 666.764 kb on - strand, within BWI76_RS03235 at 666.775 kb on + strand, within BWI76_RS03235 at 666.811 kb on + strand, within BWI76_RS03235 at 666.820 kb on + strand, within BWI76_RS03235 at 666.828 kb on - strand, within BWI76_RS03235 at 666.838 kb on - strand, within BWI76_RS03235 at 666.844 kb on - strand, within BWI76_RS03235 at 666.870 kb on + strand, within BWI76_RS03235 at 666.896 kb on - strand, within BWI76_RS03235 at 666.896 kb on - strand, within BWI76_RS03235 at 666.904 kb on - strand, within BWI76_RS03235 at 666.911 kb on + strand, within BWI76_RS03235 at 666.919 kb on - strand, within BWI76_RS03235 at 666.920 kb on - strand, within BWI76_RS03235 at 666.935 kb on - strand, within BWI76_RS03235 at 666.945 kb on - strand, within BWI76_RS03235 at 666.945 kb on - strand, within BWI76_RS03235 at 666.945 kb on - strand, within BWI76_RS03235 at 666.985 kb on - strand, within BWI76_RS03235 at 666.996 kb on - strand, within BWI76_RS03235 at 667.085 kb on - strand, within BWI76_RS03235 at 667.096 kb on - strand, within BWI76_RS03235 at 667.096 kb on - strand, within BWI76_RS03235 at 667.096 kb on - strand, within BWI76_RS03235 at 667.101 kb on - strand, within BWI76_RS03235 at 667.115 kb on + strand at 667.115 kb on + strand, within BWI76_RS03235 at 667.118 kb on - strand, within BWI76_RS03235 at 667.123 kb on - strand, within BWI76_RS03235 at 667.123 kb on - strand, within BWI76_RS03235 at 667.128 kb on - strand, within BWI76_RS03235 at 667.130 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.132 kb on + strand, within BWI76_RS03235 at 667.133 kb on - strand, within BWI76_RS03235 at 667.138 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.140 kb on - strand, within BWI76_RS03235 at 667.180 kb on + strand, within BWI76_RS03235 at 667.238 kb on + strand, within BWI76_RS03235 at 667.242 kb on + strand, within BWI76_RS03235 at 667.243 kb on + strand, within BWI76_RS03235 at 667.243 kb on + strand, within BWI76_RS03235 at 667.243 kb on + strand at 667.251 kb on - strand, within BWI76_RS03235 at 667.314 kb on + strand, within BWI76_RS03235 at 667.449 kb on + strand, within BWI76_RS03235 at 667.480 kb on + strand, within BWI76_RS03235 at 667.488 kb on - strand, within BWI76_RS03235 at 667.488 kb on - strand, within BWI76_RS03235 at 667.514 kb on + strand, within BWI76_RS03235
Per-strain Table
Position Strand Gene LocusTag Fraction rhizosphere sample 1; outgrowth in LB remove 664,627 - +0.7 664,649 + +0.4 664,649 + +0.7 664,658 + -1.1 664,660 + +0.5 664,660 + -0.8 664,660 + -2.1 664,660 + -1.3 664,660 + -0.4 664,660 + +0.2 664,660 + -1.2 664,660 + -0.5 664,660 + +1.3 664,660 + -0.4 664,666 + -2.6 664,668 + -0.1 664,668 + +1.3 664,668 + +0.4 664,668 - -0.3 664,668 - -1.7 664,668 - -1.6 664,668 - +0.9 664,668 - -2.0 664,668 - +1.3 664,668 - -0.8 664,668 - -0.9 664,668 - -3.0 664,668 - -2.1 664,668 - +0.7 664,668 - -0.1 664,668 - -2.0 664,668 - -1.5 664,668 - -0.9 664,668 - +0.8 664,671 + -0.6 664,679 - +2.0 664,679 - -1.0 664,681 + -0.6 664,690 + -0.2 664,696 - -1.3 664,700 + +2.0 664,719 + -0.6 664,738 + +0.4 664,738 + +1.2 664,748 - +3.8 664,748 - -1.4 664,793 - -1.7 664,795 + -2.4 664,798 - -2.8 664,820 + -0.1 664,836 - -0.3 665,006 - BWI76_RS03225 0.30 -0.6 665,091 + BWI76_RS03225 0.41 +0.3 665,100 + BWI76_RS03225 0.42 +1.0 665,100 + BWI76_RS03225 0.42 +0.3 665,108 - -0.1 665,108 - BWI76_RS03225 0.43 -3.0 665,130 - BWI76_RS03225 0.45 +0.0 665,130 - BWI76_RS03225 0.45 +0.1 665,155 - BWI76_RS03225 0.48 +0.8 665,155 - BWI76_RS03225 0.48 -0.9 665,155 - BWI76_RS03225 0.48 -0.4 665,155 - BWI76_RS03225 0.48 -0.5 665,158 + BWI76_RS03225 0.49 -1.5 665,190 - BWI76_RS03225 0.53 -2.0 665,261 - BWI76_RS03225 0.62 -1.5 665,261 - BWI76_RS03225 0.62 +0.1 665,275 + BWI76_RS03225 0.63 +0.1 665,279 - BWI76_RS03225 0.64 +1.3 665,307 - BWI76_RS03225 0.67 +0.6 665,443 - BWI76_RS03225 0.84 +2.5 665,445 - BWI76_RS03225 0.84 -1.3 665,476 - BWI76_RS03225 0.88 -1.4 665,477 - BWI76_RS03225 0.88 -0.6 665,513 - -0.2 665,513 - -0.9 665,513 - -1.0 665,513 - -2.5 665,513 - -1.9 665,543 - +0.4 665,543 - -1.9 665,543 - -1.1 665,559 - -3.2 665,579 + -1.2 665,580 - +0.4 665,580 - +0.9 665,587 - -1.5 665,630 + -1.6 665,630 + -1.0 665,630 + +3.6 665,630 + -2.6 665,630 + -1.3 665,630 + -0.7 665,630 + -0.9 665,630 + +0.5 665,630 + +3.6 665,630 + +1.0 665,630 + +1.0 665,630 + -1.1 665,630 + +0.3 665,630 + +1.0 665,630 + +0.0 665,631 + -1.0 665,635 + -0.0 665,635 + -0.7 665,638 - +3.6 665,638 - -2.4 665,638 - -1.5 665,638 - +2.6 665,638 - +0.2 665,638 - -2.5 665,638 - -0.7 665,638 - +0.8 665,638 - -2.1 665,638 - -0.4 665,638 - +1.6 665,638 - -1.3 665,638 - +0.3 665,640 - -1.6 665,645 - +2.0 665,648 - +0.9 665,648 - -1.2 665,648 - +2.6 665,662 + -2.9 665,677 - +0.7 665,739 + BWI76_RS03230 0.13 +0.4 665,753 - BWI76_RS03230 0.15 +0.1 665,753 - BWI76_RS03230 0.15 +1.6 665,775 + BWI76_RS03230 0.17 +1.6 665,805 - BWI76_RS03230 0.21 +3.6 665,812 - BWI76_RS03230 0.21 -2.6 665,841 + BWI76_RS03230 0.25 +1.6 665,843 + BWI76_RS03230 0.25 +2.0 665,848 + BWI76_RS03230 0.25 +0.6 665,848 + BWI76_RS03230 0.25 -1.4 665,848 + BWI76_RS03230 0.25 -1.7 665,851 - BWI76_RS03230 0.26 +0.3 665,874 - BWI76_RS03230 0.28 +2.0 665,875 + BWI76_RS03230 0.28 -0.2 665,878 - BWI76_RS03230 0.29 +2.0 665,904 + BWI76_RS03230 0.31 -0.6 665,912 - BWI76_RS03230 0.32 -1.0 665,932 + BWI76_RS03230 0.35 +2.6 665,943 - BWI76_RS03230 0.36 -2.2 666,032 + BWI76_RS03230 0.46 +0.8 666,040 - BWI76_RS03230 0.46 +0.8 666,087 - BWI76_RS03230 0.52 +1.0 666,136 + BWI76_RS03230 0.57 +1.6 666,161 + BWI76_RS03230 0.60 -0.4 666,201 - BWI76_RS03230 0.64 +2.0 666,214 - BWI76_RS03230 0.66 +2.6 666,237 + BWI76_RS03230 0.68 +0.6 666,238 - BWI76_RS03230 0.68 +3.6 666,303 + BWI76_RS03230 0.75 -2.2 666,303 + BWI76_RS03230 0.75 +2.6 666,316 + BWI76_RS03230 0.77 +1.6 666,319 - BWI76_RS03230 0.77 -0.5 666,326 - BWI76_RS03230 0.78 -0.2 666,326 - BWI76_RS03230 0.78 -1.0 666,330 + BWI76_RS03230 0.78 +2.6 666,330 + BWI76_RS03230 0.78 +3.6 666,330 + BWI76_RS03230 0.78 +0.0 666,330 + BWI76_RS03230 0.78 +1.6 666,333 - BWI76_RS03230 0.79 +1.6 666,335 + BWI76_RS03230 0.79 -2.0 666,335 + BWI76_RS03230 0.79 +2.0 666,335 + BWI76_RS03230 0.79 +2.6 666,335 + BWI76_RS03230 0.79 +1.0 666,338 - BWI76_RS03230 0.79 -0.2 666,338 - BWI76_RS03230 0.79 +0.0 666,338 - BWI76_RS03230 0.79 -2.6 666,338 - BWI76_RS03230 0.79 -1.8 666,338 - BWI76_RS03230 0.79 +0.2 666,343 - BWI76_RS03230 0.80 -1.9 666,343 - BWI76_RS03230 0.80 -1.5 666,343 - BWI76_RS03230 0.80 +0.8 666,343 - BWI76_RS03230 0.80 -1.6 666,406 + BWI76_RS03230 0.87 +0.6 666,415 - BWI76_RS03230 0.88 -0.3 666,415 - BWI76_RS03230 0.88 -2.4 666,415 - BWI76_RS03230 0.88 +3.6 666,416 - BWI76_RS03230 0.88 +0.1 666,436 - -0.6 666,442 - +1.0 666,451 - -1.2 666,451 - -1.0 666,518 - +1.3 666,528 + -0.7 666,549 - -0.6 666,564 - -0.3 666,570 - +0.8 666,571 + +2.0 666,579 - -1.5 666,583 + +1.6 666,585 + +2.6 666,585 + +0.1 666,591 - +0.9 666,593 - -1.7 666,593 - -1.1 666,593 - +0.5 666,593 - -2.4 666,638 + -0.6 666,654 - -2.0 666,764 - BWI76_RS03235 0.14 -1.6 666,775 + BWI76_RS03235 0.14 +3.3 666,811 + BWI76_RS03235 0.16 +0.0 666,820 + BWI76_RS03235 0.17 +0.8 666,828 - BWI76_RS03235 0.17 +0.5 666,838 - BWI76_RS03235 0.18 +0.4 666,844 - BWI76_RS03235 0.18 -0.1 666,870 + BWI76_RS03235 0.19 +0.6 666,896 - BWI76_RS03235 0.21 -0.8 666,896 - BWI76_RS03235 0.21 -3.3 666,904 - BWI76_RS03235 0.21 -1.2 666,911 + BWI76_RS03235 0.22 +1.3 666,919 - BWI76_RS03235 0.22 -0.6 666,920 - BWI76_RS03235 0.22 -0.1 666,935 - BWI76_RS03235 0.23 -1.0 666,945 - BWI76_RS03235 0.24 +3.6 666,945 - BWI76_RS03235 0.24 -2.3 666,945 - BWI76_RS03235 0.24 +0.8 666,985 - BWI76_RS03235 0.26 -0.0 666,996 - BWI76_RS03235 0.26 +0.4 667,085 - BWI76_RS03235 0.31 +0.0 667,096 - BWI76_RS03235 0.32 -1.4 667,096 - BWI76_RS03235 0.32 -0.3 667,096 - BWI76_RS03235 0.32 -1.9 667,101 - BWI76_RS03235 0.32 -3.0 667,115 + +3.6 667,115 + BWI76_RS03235 0.33 -1.1 667,118 - BWI76_RS03235 0.33 +0.1 667,123 - BWI76_RS03235 0.34 -1.6 667,123 - BWI76_RS03235 0.34 -0.5 667,128 - BWI76_RS03235 0.34 -1.5 667,130 + BWI76_RS03235 0.34 +2.0 667,132 + BWI76_RS03235 0.34 +3.6 667,132 + BWI76_RS03235 0.34 +3.6 667,132 + BWI76_RS03235 0.34 +3.6 667,132 + BWI76_RS03235 0.34 +3.6 667,133 - BWI76_RS03235 0.34 -0.7 667,138 - BWI76_RS03235 0.34 -2.8 667,140 - BWI76_RS03235 0.34 -0.3 667,140 - BWI76_RS03235 0.34 +1.6 667,140 - BWI76_RS03235 0.34 -0.4 667,140 - BWI76_RS03235 0.34 -1.1 667,140 - BWI76_RS03235 0.34 -0.5 667,180 + BWI76_RS03235 0.37 +0.4 667,238 + BWI76_RS03235 0.40 +2.3 667,242 + BWI76_RS03235 0.40 +1.3 667,243 + BWI76_RS03235 0.40 +2.0 667,243 + BWI76_RS03235 0.40 -0.3 667,243 + +3.0 667,251 - BWI76_RS03235 0.41 -2.0 667,314 + BWI76_RS03235 0.44 -1.0 667,449 + BWI76_RS03235 0.52 +1.5 667,480 + BWI76_RS03235 0.53 +1.0 667,488 - BWI76_RS03235 0.54 +1.3 667,488 - BWI76_RS03235 0.54 -0.9 667,514 + BWI76_RS03235 0.55 +1.6
Or see this region's nucleotide sequence