Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01525

Experiment: rhizosphere sample 1; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01510 and BWI76_RS01515 are separated by 37 nucleotidesBWI76_RS01515 and BWI76_RS01520 are separated by 3 nucleotidesBWI76_RS01520 and BWI76_RS01525 are separated by 172 nucleotidesBWI76_RS01525 and BWI76_RS01530 are separated by 318 nucleotides BWI76_RS01510: BWI76_RS01510 - anaerobic C4-dicarboxylate transporter, at 308,526 to 309,866 _RS01510 BWI76_RS01515: BWI76_RS01515 - PTS family cellobiose transporter subunit IIA, at 309,904 to 310,221 _RS01515 BWI76_RS01520: BWI76_RS01520 - PTS sugar transporter subunit IIB, at 310,225 to 310,530 _RS01520 BWI76_RS01525: BWI76_RS01525 - sensor domain-containing diguanylate cyclase, at 310,703 to 312,235 _RS01525 BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687 _RS01530 Position (kb) 310 311 312 313Strain fitness (log2 ratio) -2 -1 0 1 2 3at 309.747 kb on + strandat 309.976 kb on + strand, within BWI76_RS01515at 309.981 kb on + strand, within BWI76_RS01515at 310.030 kb on + strand, within BWI76_RS01515at 310.030 kb on + strand, within BWI76_RS01515at 310.038 kb on - strand, within BWI76_RS01515at 310.178 kb on + strand, within BWI76_RS01515at 310.237 kb on + strandat 310.325 kb on + strand, within BWI76_RS01520at 310.325 kb on + strand, within BWI76_RS01520at 310.325 kb on + strand, within BWI76_RS01520at 310.325 kb on + strand, within BWI76_RS01520at 310.333 kb on - strand, within BWI76_RS01520at 310.359 kb on + strand, within BWI76_RS01520at 310.518 kb on - strandat 310.548 kb on - strandat 310.616 kb on - strandat 310.695 kb on - strandat 310.810 kb on - strandat 311.006 kb on - strand, within BWI76_RS01525at 311.006 kb on - strand, within BWI76_RS01525at 311.170 kb on - strand, within BWI76_RS01525at 311.205 kb on - strand, within BWI76_RS01525at 311.293 kb on - strand, within BWI76_RS01525at 311.394 kb on - strand, within BWI76_RS01525at 311.450 kb on + strand, within BWI76_RS01525at 311.484 kb on + strand, within BWI76_RS01525at 311.527 kb on - strand, within BWI76_RS01525at 311.588 kb on + strand, within BWI76_RS01525at 311.657 kb on - strand, within BWI76_RS01525at 311.679 kb on - strand, within BWI76_RS01525at 311.712 kb on + strand, within BWI76_RS01525at 311.720 kb on - strand, within BWI76_RS01525at 311.722 kb on + strand, within BWI76_RS01525at 311.742 kb on - strand, within BWI76_RS01525at 311.763 kb on + strand, within BWI76_RS01525at 311.781 kb on - strand, within BWI76_RS01525at 311.840 kb on + strand, within BWI76_RS01525at 312.239 kb on - strandat 312.259 kb on + strandat 312.265 kb on - strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.282 kb on + strandat 312.290 kb on - strandat 312.290 kb on - strandat 312.290 kb on - strandat 312.290 kb on - strandat 312.290 kb on - strandat 312.290 kb on - strandat 312.290 kb on - strandat 312.337 kb on - strandat 312.392 kb on + strandat 312.399 kb on + strandat 312.406 kb on - strandat 312.416 kb on - strandat 312.435 kb on + strandat 312.443 kb on - strandat 312.443 kb on - strandat 312.452 kb on + strandat 312.460 kb on - strandat 312.462 kb on + strandat 312.488 kb on + strandat 312.562 kb on + strandat 312.570 kb on - strandat 312.570 kb on - strandat 312.570 kb on - strandat 312.577 kb on + strandat 312.587 kb on + strandat 312.596 kb on - strandat 312.643 kb on + strandat 312.666 kb on + strandat 312.674 kb on - strand, within BWI76_RS01530at 312.674 kb on - strand, within BWI76_RS01530at 312.941 kb on + strand, within BWI76_RS01530at 313.038 kb on + strand, within BWI76_RS01530

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Per-strain Table

Position Strand Gene LocusTag Fraction rhizosphere sample 1; outgrowth in LB
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309,747 + -0.9
309,976 + BWI76_RS01515 0.23 +0.6
309,981 + BWI76_RS01515 0.24 -1.2
310,030 + BWI76_RS01515 0.40 -0.9
310,030 + BWI76_RS01515 0.40 -1.4
310,038 - BWI76_RS01515 0.42 +3.4
310,178 + BWI76_RS01515 0.86 -1.1
310,237 + +1.0
310,325 + BWI76_RS01520 0.33 -1.5
310,325 + BWI76_RS01520 0.33 -0.4
310,325 + BWI76_RS01520 0.33 +0.9
310,325 + BWI76_RS01520 0.33 -0.1
310,333 - BWI76_RS01520 0.35 -0.5
310,359 + BWI76_RS01520 0.44 +0.0
310,518 - -0.2
310,548 - +0.4
310,616 - +2.1
310,695 - +0.0
310,810 - +0.8
311,006 - BWI76_RS01525 0.20 +2.1
311,006 - BWI76_RS01525 0.20 +0.1
311,170 - BWI76_RS01525 0.30 +0.4
311,205 - BWI76_RS01525 0.33 +1.4
311,293 - BWI76_RS01525 0.38 +0.0
311,394 - BWI76_RS01525 0.45 +2.3
311,450 + BWI76_RS01525 0.49 -0.3
311,484 + BWI76_RS01525 0.51 +0.2
311,527 - BWI76_RS01525 0.54 -0.3
311,588 + BWI76_RS01525 0.58 -0.1
311,657 - BWI76_RS01525 0.62 -1.5
311,679 - BWI76_RS01525 0.64 +0.0
311,712 + BWI76_RS01525 0.66 +1.6
311,720 - BWI76_RS01525 0.66 -0.7
311,722 + BWI76_RS01525 0.66 +1.5
311,742 - BWI76_RS01525 0.68 -1.8
311,763 + BWI76_RS01525 0.69 -0.4
311,781 - BWI76_RS01525 0.70 +2.4
311,840 + BWI76_RS01525 0.74 -0.1
312,239 - -0.1
312,259 + +0.0
312,265 - -0.5
312,282 + +1.2
312,282 + -1.0
312,282 + -2.1
312,282 + +0.1
312,282 + +1.1
312,282 + -0.1
312,282 + +2.8
312,282 + +1.0
312,282 + -0.7
312,290 - +0.2
312,290 - -0.1
312,290 - +0.1
312,290 - -0.7
312,290 - +1.0
312,290 - -0.0
312,290 - +0.1
312,337 - -0.3
312,392 + +0.7
312,399 + +0.9
312,406 - +0.3
312,416 - +0.4
312,435 + +1.5
312,443 - -1.1
312,443 - -0.0
312,452 + +0.0
312,460 - +1.1
312,462 + +0.0
312,488 + -0.3
312,562 + +0.2
312,570 - -0.0
312,570 - +0.8
312,570 - +0.0
312,577 + -0.8
312,587 + -2.4
312,596 - +1.7
312,643 + -0.8
312,666 + -0.1
312,674 - BWI76_RS01530 0.11 +0.9
312,674 - BWI76_RS01530 0.11 -1.0
312,941 + BWI76_RS01530 0.34 -0.7
313,038 + BWI76_RS01530 0.43 -0.4

Or see this region's nucleotide sequence