Experiment: BHIS with DAMPA 60 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3971 and BT3972 overlap by 23 nucleotides BT3972 and BT3973 are separated by 20 nucleotides BT3973 and tRNA-Pro are separated by 55 nucleotides tRNA-Pro and BT3974 are separated by 364 nucleotides
BT3971: BT3971 - glutathione peroxidase (NCBI ptt file), at 5,172,133 to 5,172,675
BT3971
BT3972: BT3972 - putative NTP pyrophosphohydrolase (NCBI ptt file), at 5,172,653 to 5,173,195
BT3972
BT3973: BT3973 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 5,173,216 to 5,174,436
BT3973
BT_t64: tRNA-Pro - tRNA-Pro (RefSeq), at 5,174,492 to 5,174,568
tRNA-Pro
BT3974: BT3974 - conserved hypothetical protein (NCBI ptt file), at 5,174,933 to 5,175,727
BT3974
Position (kb)
5173
5174
5175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5172.278 kb on + strand, within BT3971 at 5172.281 kb on + strand, within BT3971 at 5172.282 kb on - strand, within BT3971 at 5172.346 kb on + strand, within BT3971 at 5172.355 kb on - strand, within BT3971 at 5172.444 kb on - strand, within BT3971 at 5172.448 kb on + strand, within BT3971 at 5172.456 kb on + strand, within BT3971 at 5172.582 kb on + strand, within BT3971 at 5172.583 kb on - strand, within BT3971 at 5172.674 kb on - strand at 5172.698 kb on - strand at 5172.704 kb on + strand at 5172.705 kb on - strand at 5172.714 kb on - strand, within BT3972 at 5172.883 kb on - strand, within BT3972 at 5172.956 kb on + strand, within BT3972 at 5172.957 kb on - strand, within BT3972 at 5172.976 kb on + strand, within BT3972 at 5172.976 kb on + strand, within BT3972 at 5173.045 kb on - strand, within BT3972 at 5173.047 kb on - strand, within BT3972 at 5173.047 kb on - strand, within BT3972 at 5173.047 kb on - strand, within BT3972 at 5173.195 kb on + strand at 5173.196 kb on - strand at 5173.196 kb on - strand at 5173.196 kb on - strand at 5173.278 kb on - strand at 5173.284 kb on - strand at 5173.383 kb on - strand, within BT3973 at 5173.383 kb on - strand, within BT3973 at 5173.400 kb on - strand, within BT3973 at 5173.400 kb on - strand, within BT3973 at 5173.413 kb on + strand, within BT3973 at 5173.457 kb on - strand, within BT3973 at 5173.677 kb on + strand, within BT3973 at 5173.679 kb on + strand at 5173.960 kb on + strand, within BT3973 at 5174.050 kb on + strand, within BT3973 at 5174.357 kb on - strand at 5174.725 kb on - strand at 5174.747 kb on + strand at 5174.811 kb on + strand at 5174.870 kb on - strand at 5174.873 kb on - strand at 5174.897 kb on + strand at 5174.966 kb on - strand at 5174.968 kb on + strand at 5175.017 kb on - strand, within BT3974 at 5175.021 kb on + strand, within BT3974 at 5175.121 kb on + strand, within BT3974 at 5175.122 kb on - strand, within BT3974 at 5175.200 kb on - strand, within BT3974 at 5175.277 kb on + strand, within BT3974 at 5175.296 kb on - strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.297 kb on + strand, within BT3974 at 5175.298 kb on - strand, within BT3974 at 5175.299 kb on + strand, within BT3974 at 5175.300 kb on - strand, within BT3974 at 5175.333 kb on - strand, within BT3974 at 5175.336 kb on + strand, within BT3974 at 5175.404 kb on - strand, within BT3974
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with DAMPA 60 uM remove 5,172,278 + BT3971 0.27 +0.9 5,172,281 + BT3971 0.27 -0.3 5,172,282 - BT3971 0.27 -2.7 5,172,346 + BT3971 0.39 -2.0 5,172,355 - BT3971 0.41 -0.6 5,172,444 - BT3971 0.57 -1.0 5,172,448 + BT3971 0.58 -0.6 5,172,456 + BT3971 0.59 -1.0 5,172,582 + BT3971 0.83 +0.2 5,172,583 - BT3971 0.83 +0.3 5,172,674 - -0.3 5,172,698 - -0.5 5,172,704 + -0.5 5,172,705 - -3.2 5,172,714 - BT3972 0.11 -2.1 5,172,883 - BT3972 0.42 -1.1 5,172,956 + BT3972 0.56 -0.8 5,172,957 - BT3972 0.56 -0.9 5,172,976 + BT3972 0.59 -2.3 5,172,976 + BT3972 0.59 +0.3 5,173,045 - BT3972 0.72 -2.5 5,173,047 - BT3972 0.73 -0.5 5,173,047 - BT3972 0.73 -0.6 5,173,047 - BT3972 0.73 +0.1 5,173,195 + -0.1 5,173,196 - -1.9 5,173,196 - -0.6 5,173,196 - -0.8 5,173,278 - +0.3 5,173,284 - +0.9 5,173,383 - BT3973 0.14 -1.5 5,173,383 - BT3973 0.14 -2.3 5,173,400 - BT3973 0.15 -1.0 5,173,400 - BT3973 0.15 -1.5 5,173,413 + BT3973 0.16 -2.4 5,173,457 - BT3973 0.20 +0.3 5,173,677 + BT3973 0.38 -2.4 5,173,679 + +0.3 5,173,960 + BT3973 0.61 -2.7 5,174,050 + BT3973 0.68 -2.4 5,174,357 - -2.3 5,174,725 - -0.4 5,174,747 + -1.4 5,174,811 + -1.1 5,174,870 - -0.3 5,174,873 - +0.0 5,174,897 + -2.1 5,174,966 - -1.1 5,174,968 + -1.3 5,175,017 - BT3974 0.11 -1.7 5,175,021 + BT3974 0.11 -0.6 5,175,121 + BT3974 0.24 -0.7 5,175,122 - BT3974 0.24 +0.7 5,175,200 - BT3974 0.34 -1.9 5,175,277 + BT3974 0.43 -0.3 5,175,296 - BT3974 0.46 +0.8 5,175,297 + BT3974 0.46 +0.0 5,175,297 + BT3974 0.46 -0.7 5,175,297 + BT3974 0.46 -1.1 5,175,297 + BT3974 0.46 +0.3 5,175,298 - BT3974 0.46 +2.3 5,175,299 + BT3974 0.46 +0.3 5,175,300 - BT3974 0.46 +0.3 5,175,333 - BT3974 0.50 -0.5 5,175,336 + BT3974 0.51 -2.1 5,175,404 - BT3974 0.59 +0.2
Or see this region's nucleotide sequence