Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT3973

Experiment: BHIS with DAMPA 60 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT3971 and BT3972 overlap by 23 nucleotidesBT3972 and BT3973 are separated by 20 nucleotidesBT3973 and tRNA-Pro are separated by 55 nucleotidestRNA-Pro and BT3974 are separated by 364 nucleotides BT3971: BT3971 - glutathione peroxidase (NCBI ptt file), at 5,172,133 to 5,172,675 BT3971 BT3972: BT3972 - putative NTP pyrophosphohydrolase (NCBI ptt file), at 5,172,653 to 5,173,195 BT3972 BT3973: BT3973 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 5,173,216 to 5,174,436 BT3973 BT_t64: tRNA-Pro - tRNA-Pro (RefSeq), at 5,174,492 to 5,174,568 tRNA-Pro BT3974: BT3974 - conserved hypothetical protein (NCBI ptt file), at 5,174,933 to 5,175,727 BT3974 Position (kb) 5173 5174 5175Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5172.278 kb on + strand, within BT3971at 5172.281 kb on + strand, within BT3971at 5172.282 kb on - strand, within BT3971at 5172.346 kb on + strand, within BT3971at 5172.355 kb on - strand, within BT3971at 5172.444 kb on - strand, within BT3971at 5172.448 kb on + strand, within BT3971at 5172.456 kb on + strand, within BT3971at 5172.582 kb on + strand, within BT3971at 5172.583 kb on - strand, within BT3971at 5172.674 kb on - strandat 5172.698 kb on - strandat 5172.704 kb on + strandat 5172.705 kb on - strandat 5172.714 kb on - strand, within BT3972at 5172.883 kb on - strand, within BT3972at 5172.956 kb on + strand, within BT3972at 5172.957 kb on - strand, within BT3972at 5172.976 kb on + strand, within BT3972at 5172.976 kb on + strand, within BT3972at 5173.045 kb on - strand, within BT3972at 5173.047 kb on - strand, within BT3972at 5173.047 kb on - strand, within BT3972at 5173.047 kb on - strand, within BT3972at 5173.195 kb on + strandat 5173.196 kb on - strandat 5173.196 kb on - strandat 5173.196 kb on - strandat 5173.278 kb on - strandat 5173.284 kb on - strandat 5173.383 kb on - strand, within BT3973at 5173.383 kb on - strand, within BT3973at 5173.400 kb on - strand, within BT3973at 5173.400 kb on - strand, within BT3973at 5173.413 kb on + strand, within BT3973at 5173.457 kb on - strand, within BT3973at 5173.677 kb on + strand, within BT3973at 5173.679 kb on + strandat 5173.960 kb on + strand, within BT3973at 5174.050 kb on + strand, within BT3973at 5174.357 kb on - strandat 5174.725 kb on - strandat 5174.747 kb on + strandat 5174.811 kb on + strandat 5174.870 kb on - strandat 5174.873 kb on - strandat 5174.897 kb on + strandat 5174.966 kb on - strandat 5174.968 kb on + strandat 5175.017 kb on - strand, within BT3974at 5175.021 kb on + strand, within BT3974at 5175.121 kb on + strand, within BT3974at 5175.122 kb on - strand, within BT3974at 5175.200 kb on - strand, within BT3974at 5175.277 kb on + strand, within BT3974at 5175.296 kb on - strand, within BT3974at 5175.297 kb on + strand, within BT3974at 5175.297 kb on + strand, within BT3974at 5175.297 kb on + strand, within BT3974at 5175.297 kb on + strand, within BT3974at 5175.298 kb on - strand, within BT3974at 5175.299 kb on + strand, within BT3974at 5175.300 kb on - strand, within BT3974at 5175.333 kb on - strand, within BT3974at 5175.336 kb on + strand, within BT3974at 5175.404 kb on - strand, within BT3974

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with DAMPA 60 uM
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5,172,278 + BT3971 0.27 +0.9
5,172,281 + BT3971 0.27 -0.3
5,172,282 - BT3971 0.27 -2.7
5,172,346 + BT3971 0.39 -2.0
5,172,355 - BT3971 0.41 -0.6
5,172,444 - BT3971 0.57 -1.0
5,172,448 + BT3971 0.58 -0.6
5,172,456 + BT3971 0.59 -1.0
5,172,582 + BT3971 0.83 +0.2
5,172,583 - BT3971 0.83 +0.3
5,172,674 - -0.3
5,172,698 - -0.5
5,172,704 + -0.5
5,172,705 - -3.2
5,172,714 - BT3972 0.11 -2.1
5,172,883 - BT3972 0.42 -1.1
5,172,956 + BT3972 0.56 -0.8
5,172,957 - BT3972 0.56 -0.9
5,172,976 + BT3972 0.59 -2.3
5,172,976 + BT3972 0.59 +0.3
5,173,045 - BT3972 0.72 -2.5
5,173,047 - BT3972 0.73 -0.5
5,173,047 - BT3972 0.73 -0.6
5,173,047 - BT3972 0.73 +0.1
5,173,195 + -0.1
5,173,196 - -1.9
5,173,196 - -0.6
5,173,196 - -0.8
5,173,278 - +0.3
5,173,284 - +0.9
5,173,383 - BT3973 0.14 -1.5
5,173,383 - BT3973 0.14 -2.3
5,173,400 - BT3973 0.15 -1.0
5,173,400 - BT3973 0.15 -1.5
5,173,413 + BT3973 0.16 -2.4
5,173,457 - BT3973 0.20 +0.3
5,173,677 + BT3973 0.38 -2.4
5,173,679 + +0.3
5,173,960 + BT3973 0.61 -2.7
5,174,050 + BT3973 0.68 -2.4
5,174,357 - -2.3
5,174,725 - -0.4
5,174,747 + -1.4
5,174,811 + -1.1
5,174,870 - -0.3
5,174,873 - +0.0
5,174,897 + -2.1
5,174,966 - -1.1
5,174,968 + -1.3
5,175,017 - BT3974 0.11 -1.7
5,175,021 + BT3974 0.11 -0.6
5,175,121 + BT3974 0.24 -0.7
5,175,122 - BT3974 0.24 +0.7
5,175,200 - BT3974 0.34 -1.9
5,175,277 + BT3974 0.43 -0.3
5,175,296 - BT3974 0.46 +0.8
5,175,297 + BT3974 0.46 +0.0
5,175,297 + BT3974 0.46 -0.7
5,175,297 + BT3974 0.46 -1.1
5,175,297 + BT3974 0.46 +0.3
5,175,298 - BT3974 0.46 +2.3
5,175,299 + BT3974 0.46 +0.3
5,175,300 - BT3974 0.46 +0.3
5,175,333 - BT3974 0.50 -0.5
5,175,336 + BT3974 0.51 -2.1
5,175,404 - BT3974 0.59 +0.2

Or see this region's nucleotide sequence