Experiment: BHIS with DAMPA 60 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3212 and BT3213 are separated by 52 nucleotides BT3213 and BT3214 are separated by 17 nucleotides BT3214 and BT3215 are separated by 16 nucleotides BT3215 and BT3216 are separated by 7 nucleotides BT3216 and BT3217 are separated by 30 nucleotides
BT3212: BT3212 - putative bacitracin resistance protein (NCBI ptt file), at 4,104,984 to 4,105,778
BT3212
BT3213: BT3213 - tRNA pseudouridine synthase B (NCBI ptt file), at 4,105,831 to 4,106,553
BT3213
BT3214: BT3214 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 4,106,571 to 4,107,629
BT3214
BT3215: BT3215 - conserved hypothetical protein (NCBI ptt file), at 4,107,646 to 4,107,981
BT3215
BT3216: BT3216 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine (NCBI ptt file), at 4,107,989 to 4,108,471
BT3216
BT3217: BT3217 - hypothetical protein (NCBI ptt file), at 4,108,502 to 4,108,693
BT3217
Position (kb)
4106
4107
4108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4105.793 kb on + strand at 4105.898 kb on + strand at 4105.952 kb on + strand, within BT3213 at 4106.306 kb on + strand, within BT3213 at 4106.306 kb on + strand, within BT3213 at 4106.380 kb on + strand, within BT3213 at 4106.556 kb on + strand at 4107.684 kb on + strand, within BT3215 at 4107.684 kb on + strand at 4107.712 kb on + strand, within BT3215 at 4107.734 kb on + strand, within BT3215 at 4107.907 kb on + strand, within BT3215 at 4107.932 kb on + strand, within BT3215 at 4107.932 kb on + strand, within BT3215 at 4107.974 kb on + strand at 4107.974 kb on + strand at 4108.563 kb on - strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.572 kb on - strand, within BT3217 at 4108.583 kb on - strand, within BT3217 at 4108.613 kb on + strand, within BT3217 at 4108.614 kb on - strand, within BT3217 at 4108.624 kb on - strand, within BT3217
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with DAMPA 60 uM remove 4,105,793 + +0.4 4,105,898 + +2.9 4,105,952 + BT3213 0.17 +2.1 4,106,306 + BT3213 0.66 -0.1 4,106,306 + BT3213 0.66 +0.9 4,106,380 + BT3213 0.76 +0.7 4,106,556 + +0.1 4,107,684 + BT3215 0.11 -1.6 4,107,684 + +1.6 4,107,712 + BT3215 0.20 -1.1 4,107,734 + BT3215 0.26 -2.8 4,107,907 + BT3215 0.78 -1.2 4,107,932 + BT3215 0.85 -3.0 4,107,932 + BT3215 0.85 -1.6 4,107,974 + -2.7 4,107,974 + -2.3 4,108,563 - BT3217 0.32 -1.4 4,108,571 + BT3217 0.36 -0.7 4,108,571 + BT3217 0.36 -0.8 4,108,572 - BT3217 0.36 -1.5 4,108,583 - BT3217 0.42 -0.7 4,108,613 + BT3217 0.58 +2.5 4,108,614 - BT3217 0.58 +0.5 4,108,624 - BT3217 0.64 -1.6
Or see this region's nucleotide sequence