Experiment: C.elegans, mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoB and deoD-2 are separated by 91 nucleotides deoD-2 and SO1222 are separated by 167 nucleotides SO1222 and serB are separated by 79 nucleotides
SO1219: deoB - Phosphopentomutase (EC 5.4.2.7) (from data) , at 1,266,092 to 1,267,306
deoB
SO1221: deoD-2 - purine nucleoside phosphorylase (NCBI ptt file), at 1,267,398 to 1,268,108
deoD-2
SO1222: SO1222 - hypothetical protein (NCBI ptt file), at 1,268,276 to 1,269,499
SO1222
SO_1223: serB - phosphoserine phosphatase (RefSeq), at 1,269,579 to 1,270,571
serB
Position (kb)
1268
1269
1270 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.346 kb on - strand at 1267.412 kb on + strand at 1267.479 kb on - strand, within deoD-2 at 1267.573 kb on - strand, within deoD-2 at 1267.687 kb on - strand, within deoD-2 at 1267.699 kb on - strand, within deoD-2 at 1267.724 kb on + strand, within deoD-2 at 1267.732 kb on - strand, within deoD-2 at 1267.807 kb on - strand, within deoD-2 at 1267.965 kb on + strand, within deoD-2 at 1268.038 kb on + strand at 1268.105 kb on + strand at 1268.113 kb on - strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.116 kb on + strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.124 kb on - strand at 1268.351 kb on - strand at 1268.454 kb on + strand, within SO1222 at 1268.467 kb on - strand, within SO1222 at 1268.501 kb on + strand, within SO1222 at 1268.503 kb on + strand, within SO1222 at 1268.509 kb on - strand, within SO1222 at 1268.600 kb on - strand, within SO1222 at 1268.605 kb on + strand, within SO1222 at 1268.701 kb on - strand, within SO1222 at 1268.812 kb on + strand, within SO1222 at 1268.835 kb on + strand, within SO1222 at 1268.843 kb on - strand, within SO1222 at 1268.998 kb on - strand, within SO1222 at 1269.133 kb on + strand, within SO1222 at 1269.176 kb on + strand, within SO1222 at 1269.773 kb on + strand, within serB at 1269.818 kb on - strand, within serB at 1269.833 kb on + strand, within serB at 1269.838 kb on + strand, within serB at 1269.903 kb on + strand, within serB at 1270.109 kb on + strand, within serB at 1270.109 kb on + strand, within serB at 1270.117 kb on - strand, within serB at 1270.117 kb on - strand, within serB at 1270.125 kb on - strand, within serB at 1270.142 kb on - strand, within serB at 1270.193 kb on + strand, within serB at 1270.193 kb on + strand, within serB at 1270.193 kb on + strand, within serB at 1270.201 kb on - strand, within serB at 1270.234 kb on + strand, within serB at 1270.335 kb on - strand, within serB at 1270.358 kb on + strand, within serB at 1270.358 kb on + strand, within serB at 1270.358 kb on + strand, within serB at 1270.358 kb on + strand, within serB at 1270.366 kb on - strand, within serB at 1270.440 kb on + strand, within serB
Per-strain Table
Position Strand Gene LocusTag Fraction C.elegans, mixed culture-2 remove 1,267,346 - +0.5 1,267,346 - +0.5 1,267,346 - +1.2 1,267,346 - +2.3 1,267,412 + +2.2 1,267,479 - deoD-2 SO1221 0.11 -0.7 1,267,573 - deoD-2 SO1221 0.25 +1.1 1,267,687 - deoD-2 SO1221 0.41 +1.8 1,267,699 - deoD-2 SO1221 0.42 +0.8 1,267,724 + deoD-2 SO1221 0.46 +1.3 1,267,732 - deoD-2 SO1221 0.47 +3.2 1,267,807 - deoD-2 SO1221 0.58 -1.9 1,267,965 + deoD-2 SO1221 0.80 -0.8 1,268,038 + +0.1 1,268,105 + +0.4 1,268,113 - -2.0 1,268,116 + +0.4 1,268,116 + +1.5 1,268,116 + +1.1 1,268,116 + -0.6 1,268,116 + +0.2 1,268,124 - -0.2 1,268,124 - +0.8 1,268,124 - -0.6 1,268,124 - +0.3 1,268,124 - +1.7 1,268,124 - +1.3 1,268,124 - -0.0 1,268,124 - -0.7 1,268,124 - +0.0 1,268,124 - -1.4 1,268,124 - -1.1 1,268,124 - +1.0 1,268,351 - +0.2 1,268,454 + SO1222 0.15 -0.9 1,268,467 - SO1222 0.16 -0.3 1,268,501 + SO1222 0.18 -1.0 1,268,503 + SO1222 0.19 -0.9 1,268,509 - SO1222 0.19 -2.3 1,268,600 - SO1222 0.26 -0.6 1,268,605 + SO1222 0.27 -1.5 1,268,701 - SO1222 0.35 -1.0 1,268,812 + SO1222 0.44 -1.1 1,268,835 + SO1222 0.46 +0.0 1,268,843 - SO1222 0.46 +0.1 1,268,998 - SO1222 0.59 +2.8 1,269,133 + SO1222 0.70 +1.1 1,269,176 + SO1222 0.74 -1.6 1,269,773 + serB SO_1223 0.20 -0.5 1,269,818 - serB SO_1223 0.24 +0.2 1,269,833 + serB SO_1223 0.26 +1.6 1,269,838 + serB SO_1223 0.26 -1.9 1,269,903 + serB SO_1223 0.33 -2.5 1,270,109 + serB SO_1223 0.53 +2.6 1,270,109 + serB SO_1223 0.53 -1.5 1,270,117 - serB SO_1223 0.54 -0.6 1,270,117 - serB SO_1223 0.54 -0.8 1,270,125 - serB SO_1223 0.55 -2.4 1,270,142 - serB SO_1223 0.57 +0.1 1,270,193 + serB SO_1223 0.62 +1.8 1,270,193 + serB SO_1223 0.62 -0.6 1,270,193 + serB SO_1223 0.62 +0.6 1,270,201 - serB SO_1223 0.63 -0.2 1,270,234 + serB SO_1223 0.66 +2.7 1,270,335 - serB SO_1223 0.76 -0.3 1,270,358 + serB SO_1223 0.78 -1.0 1,270,358 + serB SO_1223 0.78 -1.8 1,270,358 + serB SO_1223 0.78 -1.1 1,270,358 + serB SO_1223 0.78 -0.5 1,270,366 - serB SO_1223 0.79 +0.2 1,270,440 + serB SO_1223 0.87 -1.3
Or see this region's nucleotide sequence