Experiment: C.elegans, mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purD and purH are separated by 62 nucleotides purH and SO0443 are separated by 335 nucleotides SO0443 and SO0444 overlap by 1 nucleotides
SO0441: purD - phosphoribosylamine--glycine ligase (NCBI ptt file), at 468,430 to 469,728
purD
SO0442: purH - phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI ptt file), at 469,791 to 471,422
purH
SO0443: SO0443 - transcriptional regulator, MerR family (NCBI ptt file), at 471,758 to 472,249
SO0443
SO0444: SO0444 - hypothetical protein (NCBI ptt file), at 472,249 to 473,724
SO0444
Position (kb) 469
470
471
472 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 468.899 kb on + strand, within purD at 468.900 kb on - strand, within purD at 468.915 kb on - strand, within purD at 468.915 kb on - strand, within purD at 468.951 kb on + strand, within purD at 469.016 kb on - strand, within purD at 469.264 kb on - strand, within purD at 469.447 kb on - strand, within purD at 469.472 kb on + strand, within purD at 469.576 kb on - strand, within purD at 469.635 kb on + strand at 469.635 kb on + strand at 469.639 kb on + strand at 469.643 kb on - strand at 469.643 kb on - strand at 469.643 kb on - strand at 469.643 kb on - strand at 469.649 kb on + strand at 469.657 kb on - strand at 469.657 kb on - strand at 469.676 kb on + strand at 469.738 kb on + strand at 469.806 kb on + strand at 469.987 kb on - strand, within purH at 469.995 kb on - strand, within purH at 470.022 kb on + strand, within purH at 470.104 kb on + strand, within purH at 470.114 kb on + strand, within purH at 470.163 kb on - strand, within purH at 470.174 kb on + strand, within purH at 470.183 kb on + strand, within purH at 470.241 kb on + strand, within purH at 470.254 kb on - strand, within purH at 470.276 kb on + strand, within purH at 470.329 kb on + strand, within purH at 470.337 kb on - strand, within purH at 470.388 kb on + strand, within purH at 470.490 kb on + strand, within purH at 470.493 kb on - strand, within purH at 470.495 kb on + strand, within purH at 470.495 kb on + strand, within purH at 470.495 kb on + strand, within purH at 470.495 kb on + strand, within purH at 470.495 kb on + strand, within purH at 470.511 kb on - strand, within purH at 470.517 kb on - strand, within purH at 470.703 kb on - strand, within purH at 470.786 kb on + strand, within purH at 470.852 kb on - strand, within purH at 470.878 kb on - strand, within purH at 470.979 kb on + strand, within purH at 471.053 kb on + strand, within purH at 471.094 kb on + strand, within purH at 471.094 kb on + strand, within purH at 471.094 kb on + strand, within purH at 471.102 kb on - strand, within purH at 471.171 kb on + strand, within purH at 471.179 kb on - strand, within purH at 471.213 kb on - strand, within purH at 471.236 kb on + strand, within purH at 471.239 kb on + strand, within purH at 471.244 kb on - strand, within purH at 471.244 kb on - strand, within purH at 471.263 kb on + strand at 471.285 kb on + strand at 471.314 kb on + strand at 471.314 kb on + strand at 471.314 kb on + strand at 471.314 kb on + strand at 471.383 kb on - strand at 471.420 kb on + strand at 471.612 kb on + strand at 471.676 kb on + strand at 471.715 kb on + strand at 471.742 kb on + strand at 471.757 kb on - strand at 471.757 kb on - strand at 471.757 kb on - strand at 471.801 kb on - strand at 471.879 kb on - strand, within SO0443 at 471.879 kb on - strand, within SO0443 at 471.891 kb on + strand, within SO0443 at 471.993 kb on + strand, within SO0443 at 472.006 kb on + strand, within SO0443 at 472.014 kb on - strand, within SO0443 at 472.017 kb on + strand, within SO0443 at 472.286 kb on - strand at 472.383 kb on + strand at 472.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction C.elegans, mixed culture-2 remove 468,899 + purD SO0441 0.36 -2.1 468,900 - purD SO0441 0.36 -3.1 468,915 - purD SO0441 0.37 -1.9 468,915 - purD SO0441 0.37 -0.3 468,951 + purD SO0441 0.40 -1.5 469,016 - purD SO0441 0.45 +0.0 469,264 - purD SO0441 0.64 +0.0 469,447 - purD SO0441 0.78 -1.3 469,472 + purD SO0441 0.80 -1.1 469,576 - purD SO0441 0.88 -1.7 469,635 + -2.7 469,635 + -2.1 469,639 + -1.0 469,643 - -0.6 469,643 - -1.0 469,643 - -2.8 469,643 - -2.0 469,649 + -1.5 469,657 - -1.7 469,657 - +1.6 469,676 + -1.6 469,738 + -1.9 469,806 + -1.7 469,987 - purH SO0442 0.12 -1.2 469,995 - purH SO0442 0.12 -2.8 470,022 + purH SO0442 0.14 -2.2 470,104 + purH SO0442 0.19 -1.5 470,114 + purH SO0442 0.20 -0.8 470,163 - purH SO0442 0.23 +1.6 470,174 + purH SO0442 0.23 -2.8 470,183 + purH SO0442 0.24 -3.1 470,241 + purH SO0442 0.28 -1.0 470,254 - purH SO0442 0.28 +0.0 470,276 + purH SO0442 0.30 -1.7 470,329 + purH SO0442 0.33 -2.1 470,337 - purH SO0442 0.33 -0.4 470,388 + purH SO0442 0.37 -0.5 470,490 + purH SO0442 0.43 -2.6 470,493 - purH SO0442 0.43 -2.2 470,495 + purH SO0442 0.43 -3.8 470,495 + purH SO0442 0.43 -0.8 470,495 + purH SO0442 0.43 -1.3 470,495 + purH SO0442 0.43 -0.4 470,495 + purH SO0442 0.43 -0.1 470,511 - purH SO0442 0.44 -2.6 470,517 - purH SO0442 0.44 -2.2 470,703 - purH SO0442 0.56 -1.3 470,786 + purH SO0442 0.61 +0.6 470,852 - purH SO0442 0.65 -0.8 470,878 - purH SO0442 0.67 -2.7 470,979 + purH SO0442 0.73 -3.5 471,053 + purH SO0442 0.77 -0.2 471,094 + purH SO0442 0.80 -1.3 471,094 + purH SO0442 0.80 -1.1 471,094 + purH SO0442 0.80 -2.0 471,102 - purH SO0442 0.80 -0.5 471,171 + purH SO0442 0.85 +0.6 471,179 - purH SO0442 0.85 -1.5 471,213 - purH SO0442 0.87 -1.0 471,236 + purH SO0442 0.89 +0.0 471,239 + purH SO0442 0.89 +0.2 471,244 - purH SO0442 0.89 -1.7 471,244 - purH SO0442 0.89 -1.1 471,263 + -1.7 471,285 + -2.0 471,314 + -1.5 471,314 + -2.3 471,314 + -1.1 471,314 + -1.7 471,383 - -1.6 471,420 + -2.4 471,612 + -0.0 471,676 + +0.7 471,715 + +0.9 471,742 + -0.8 471,757 - -0.4 471,757 - -0.4 471,757 - +0.4 471,801 - -0.4 471,879 - SO0443 0.25 +0.9 471,879 - SO0443 0.25 -0.1 471,891 + SO0443 0.27 +0.3 471,993 + SO0443 0.48 -1.8 472,006 + SO0443 0.50 +2.0 472,014 - SO0443 0.52 +0.6 472,017 + SO0443 0.53 +0.5 472,286 - +1.1 472,383 + -1.1 472,391 - +1.0
Or see this region's nucleotide sequence