Experiment: C.elegans, mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspI and gspJ overlap by 20 nucleotides gspJ and gspK overlap by 4 nucleotides gspK and gspL are separated by 48 nucleotides
SO0171: gspI - general secretion pathway protein I (NCBI ptt file), at 178,274 to 178,642
gspI
SO0172: gspJ - general secretion pathway protein J (NCBI ptt file), at 178,623 to 179,390
gspJ
SO0173: gspK - general secretion pathway protein K (NCBI ptt file), at 179,387 to 180,385
gspK
SO0174: gspL - general secretion pathway protein L (NCBI ptt file), at 180,434 to 181,621
gspL
Position (kb) 179
180
181 Strain fitness (log2 ratio) -2
-1
0
1
2 at 178.440 kb on + strand, within gspI at 178.516 kb on - strand, within gspI at 178.605 kb on - strand, within gspI at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.654 kb on + strand at 178.662 kb on - strand at 178.662 kb on - strand at 178.700 kb on + strand, within gspJ at 178.730 kb on - strand, within gspJ at 178.742 kb on - strand, within gspJ at 178.744 kb on + strand, within gspJ at 178.816 kb on + strand, within gspJ at 178.824 kb on - strand, within gspJ at 178.849 kb on - strand, within gspJ at 178.850 kb on + strand, within gspJ at 178.867 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.943 kb on + strand, within gspJ at 178.950 kb on - strand, within gspJ at 178.951 kb on - strand, within gspJ at 179.018 kb on - strand, within gspJ at 179.151 kb on + strand, within gspJ at 179.157 kb on + strand, within gspJ at 179.220 kb on - strand, within gspJ at 179.273 kb on + strand, within gspJ at 179.303 kb on - strand, within gspJ at 179.394 kb on + strand at 179.436 kb on - strand at 179.544 kb on - strand, within gspK at 179.635 kb on + strand, within gspK at 179.645 kb on + strand, within gspK at 179.785 kb on + strand, within gspK at 179.849 kb on - strand, within gspK at 179.892 kb on + strand, within gspK at 180.055 kb on + strand, within gspK at 180.102 kb on - strand, within gspK at 180.209 kb on - strand, within gspK at 180.410 kb on + strand at 180.418 kb on - strand at 180.424 kb on + strand at 180.476 kb on - strand at 180.493 kb on + strand at 180.538 kb on + strand at 180.546 kb on - strand at 180.546 kb on - strand at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.569 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.628 kb on - strand, within gspL at 180.697 kb on - strand, within gspL at 180.910 kb on - strand, within gspL at 180.920 kb on + strand, within gspL at 180.932 kb on + strand, within gspL at 181.035 kb on + strand, within gspL at 181.078 kb on - strand, within gspL at 181.147 kb on - strand, within gspL at 181.225 kb on - strand, within gspL at 181.281 kb on - strand, within gspL at 181.311 kb on - strand, within gspL at 181.364 kb on + strand, within gspL at 181.364 kb on + strand, within gspL at 181.372 kb on - strand, within gspL at 181.372 kb on - strand, within gspL
Per-strain Table
Position Strand Gene LocusTag Fraction C.elegans, mixed culture-2 remove 178,440 + gspI SO0171 0.45 -0.3 178,516 - gspI SO0171 0.66 +0.6 178,605 - gspI SO0171 0.90 +1.6 178,654 + +0.6 178,654 + -0.3 178,654 + -1.3 178,654 + +1.0 178,654 + +1.6 178,654 + -0.3 178,662 - -0.8 178,662 - -0.3 178,700 + gspJ SO0172 0.10 +0.6 178,730 - gspJ SO0172 0.14 -1.1 178,742 - gspJ SO0172 0.15 +1.0 178,744 + gspJ SO0172 0.16 +1.6 178,816 + gspJ SO0172 0.25 +1.0 178,824 - gspJ SO0172 0.26 -0.3 178,849 - gspJ SO0172 0.29 +0.2 178,850 + gspJ SO0172 0.30 +1.2 178,867 + gspJ SO0172 0.32 +1.6 178,943 + gspJ SO0172 0.42 +0.6 178,943 + gspJ SO0172 0.42 +2.0 178,950 - gspJ SO0172 0.43 +2.6 178,951 - gspJ SO0172 0.43 +1.6 179,018 - gspJ SO0172 0.51 +0.2 179,151 + gspJ SO0172 0.69 -0.3 179,157 + gspJ SO0172 0.70 +2.6 179,220 - gspJ SO0172 0.78 +1.6 179,273 + gspJ SO0172 0.85 -2.3 179,303 - gspJ SO0172 0.89 -0.0 179,394 + +0.2 179,436 - +0.6 179,544 - gspK SO0173 0.16 -1.0 179,635 + gspK SO0173 0.25 -1.0 179,645 + gspK SO0173 0.26 +0.2 179,785 + gspK SO0173 0.40 +1.0 179,849 - gspK SO0173 0.46 +1.0 179,892 + gspK SO0173 0.51 -0.2 180,055 + gspK SO0173 0.67 -0.0 180,102 - gspK SO0173 0.72 +1.6 180,209 - gspK SO0173 0.82 +1.6 180,410 + +0.6 180,418 - +0.6 180,424 + -0.3 180,476 - +0.2 180,493 + +1.6 180,538 + +0.6 180,546 - +1.6 180,546 - +2.6 180,569 - gspL SO0174 0.11 +2.6 180,569 - gspL SO0174 0.11 +0.6 180,569 - gspL SO0174 0.11 +2.6 180,628 - gspL SO0174 0.16 +0.6 180,628 - gspL SO0174 0.16 -0.6 180,697 - gspL SO0174 0.22 +2.6 180,910 - gspL SO0174 0.40 +0.1 180,920 + gspL SO0174 0.41 +0.6 180,932 + gspL SO0174 0.42 +0.6 181,035 + gspL SO0174 0.51 +1.8 181,078 - gspL SO0174 0.54 -0.6 181,147 - gspL SO0174 0.60 +2.0 181,225 - gspL SO0174 0.67 -0.0 181,281 - gspL SO0174 0.71 +1.0 181,311 - gspL SO0174 0.74 +2.6 181,364 + gspL SO0174 0.78 +0.6 181,364 + gspL SO0174 0.78 +2.6 181,372 - gspL SO0174 0.79 +1.0 181,372 - gspL SO0174 0.79 +1.6
Or see this region's nucleotide sequence