Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt umuC and umuD are separated by 0 nucleotides umuD and SOA0014 are separated by 592 nucleotides SOA0014 and SOA0015 are separated by 304 nucleotides SOA0015 and SOA0016 are separated by 66 nucleotides
SOA0012: umuC - umuC protein (NCBI ptt file), at 10,078 to 11,334
umuC
SOA0013: umuD - umuD protein (VIMSS), at 11,335 to 11,739
umuD
SOA0014: SOA0014 - hypothetical protein (NCBI ptt file), at 12,332 to 12,427
SOA0014
SOA0015: SOA0015 - hypothetical protein (NCBI ptt file), at 12,732 to 12,878
SOA0015
SOA0016: SOA0016 - ISSod9, transposase (NCBI ptt file), at 12,945 to 15,911
SOA0016
Position (kb)
12
13 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 11.332 kb on + strand at 11.391 kb on - strand, within umuD at 11.452 kb on - strand, within umuD at 11.471 kb on - strand, within umuD at 11.585 kb on - strand, within umuD at 11.654 kb on + strand, within umuD at 11.660 kb on + strand, within umuD at 11.668 kb on - strand, within umuD at 11.678 kb on - strand, within umuD at 11.682 kb on - strand, within umuD at 11.913 kb on + strand at 12.111 kb on + strand at 12.111 kb on + strand at 12.113 kb on + strand at 12.119 kb on - strand at 12.120 kb on - strand at 12.120 kb on - strand at 12.204 kb on - strand at 12.293 kb on + strand at 12.301 kb on - strand at 12.301 kb on - strand at 12.303 kb on + strand at 12.330 kb on - strand at 12.359 kb on - strand, within SOA0014 at 12.361 kb on + strand, within SOA0014 at 12.561 kb on + strand at 12.569 kb on - strand at 12.592 kb on + strand at 12.686 kb on + strand at 12.686 kb on + strand at 12.904 kb on + strand at 12.912 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 11,332 + -0.5 11,391 - umuD SOA0013 0.14 -0.2 11,452 - umuD SOA0013 0.29 +0.1 11,471 - umuD SOA0013 0.34 -0.7 11,585 - umuD SOA0013 0.62 +0.3 11,654 + umuD SOA0013 0.79 -1.3 11,660 + umuD SOA0013 0.80 -0.3 11,668 - umuD SOA0013 0.82 -2.4 11,678 - umuD SOA0013 0.85 +0.9 11,682 - umuD SOA0013 0.86 +0.6 11,913 + -1.3 12,111 + -0.4 12,111 + -0.9 12,113 + -0.9 12,119 - +1.1 12,120 - +0.9 12,120 - +0.8 12,204 - -0.0 12,293 + +0.5 12,301 - -0.9 12,301 - -0.5 12,303 + -1.4 12,330 - +0.3 12,359 - SOA0014 0.28 -3.8 12,361 + SOA0014 0.30 -1.4 12,561 + -0.4 12,569 - +1.5 12,592 + +0.4 12,686 + +0.3 12,686 + -0.2 12,904 + +0.2 12,912 - -0.8
Or see this region's nucleotide sequence