Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpC/F and trpB are separated by 119 nucleotides trpB and trpA are separated by 5 nucleotides trpA and SO3025 are separated by 90 nucleotides
SO3022: trpC/F - indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (NCBI ptt file), at 3,133,337 to 3,134,746
trpC/F
SO3023: trpB - tryptophan synthase, beta subunit (NCBI ptt file), at 3,134,866 to 3,136,056
trpB
SO3024: trpA - tryptophan synthase, alpha subunit (NCBI ptt file), at 3,136,062 to 3,136,898
trpA
SO3025: SO3025 - conserved hypothetical protein (NCBI ptt file), at 3,136,989 to 3,137,981
SO3025
Position (kb)
3134
3135
3136
3137 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3133.945 kb on + strand, within trpC/F at 3134.058 kb on + strand, within trpC/F at 3134.139 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.257 kb on - strand, within trpC/F at 3134.345 kb on + strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.399 kb on - strand, within trpC/F at 3134.402 kb on + strand, within trpC/F at 3134.431 kb on - strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.496 kb on - strand, within trpC/F at 3134.552 kb on - strand, within trpC/F at 3134.703 kb on + strand at 3134.797 kb on + strand at 3134.850 kb on + strand at 3134.880 kb on - strand at 3134.996 kb on - strand, within trpB at 3134.996 kb on - strand, within trpB at 3135.032 kb on + strand, within trpB at 3135.111 kb on - strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.213 kb on - strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.234 kb on - strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.264 kb on + strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.398 kb on + strand, within trpB at 3135.624 kb on + strand, within trpB at 3135.651 kb on - strand, within trpB at 3135.659 kb on + strand, within trpB at 3135.775 kb on + strand, within trpB at 3135.775 kb on + strand, within trpB at 3135.783 kb on - strand, within trpB at 3135.805 kb on - strand, within trpB at 3135.805 kb on - strand, within trpB at 3135.933 kb on + strand, within trpB at 3135.941 kb on - strand at 3135.943 kb on - strand at 3136.061 kb on + strand at 3136.092 kb on + strand at 3136.150 kb on + strand, within trpA at 3136.160 kb on - strand, within trpA at 3136.183 kb on + strand, within trpA at 3136.191 kb on - strand, within trpA at 3136.191 kb on - strand, within trpA at 3136.218 kb on + strand, within trpA at 3136.242 kb on + strand, within trpA at 3136.339 kb on + strand, within trpA at 3136.390 kb on + strand, within trpA at 3136.390 kb on + strand, within trpA at 3136.409 kb on + strand, within trpA at 3136.417 kb on - strand, within trpA at 3136.417 kb on - strand, within trpA at 3136.530 kb on + strand, within trpA at 3136.532 kb on - strand, within trpA at 3136.532 kb on - strand, within trpA at 3136.538 kb on - strand, within trpA at 3136.610 kb on + strand, within trpA at 3136.618 kb on - strand, within trpA at 3136.722 kb on + strand, within trpA at 3136.757 kb on - strand, within trpA at 3136.757 kb on - strand, within trpA at 3136.987 kb on + strand at 3136.987 kb on + strand at 3137.004 kb on + strand at 3137.004 kb on + strand at 3137.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 3,133,945 + trpC/F SO3022 0.43 -0.3 3,134,058 + trpC/F SO3022 0.51 -1.3 3,134,139 + trpC/F SO3022 0.57 -0.5 3,134,249 + trpC/F SO3022 0.65 -2.0 3,134,249 + trpC/F SO3022 0.65 -1.5 3,134,257 - trpC/F SO3022 0.65 -1.0 3,134,345 + trpC/F SO3022 0.71 -2.8 3,134,365 - trpC/F SO3022 0.73 -1.9 3,134,365 - trpC/F SO3022 0.73 -2.0 3,134,399 - trpC/F SO3022 0.75 -2.3 3,134,402 + trpC/F SO3022 0.76 -0.3 3,134,431 - trpC/F SO3022 0.78 -1.6 3,134,454 + trpC/F SO3022 0.79 -1.4 3,134,454 + trpC/F SO3022 0.79 -2.2 3,134,496 - trpC/F SO3022 0.82 -2.7 3,134,552 - trpC/F SO3022 0.86 +1.1 3,134,703 + -0.3 3,134,797 + -1.3 3,134,850 + -0.9 3,134,880 - -2.4 3,134,996 - trpB SO3023 0.11 -1.0 3,134,996 - trpB SO3023 0.11 -0.9 3,135,032 + trpB SO3023 0.14 -1.4 3,135,111 - trpB SO3023 0.21 -1.2 3,135,139 + trpB SO3023 0.23 +2.8 3,135,139 + trpB SO3023 0.23 -2.0 3,135,205 + trpB SO3023 0.28 -2.1 3,135,205 + trpB SO3023 0.28 -1.9 3,135,213 - trpB SO3023 0.29 -1.3 3,135,226 + trpB SO3023 0.30 -2.5 3,135,226 + trpB SO3023 0.30 -0.9 3,135,234 - trpB SO3023 0.31 +0.0 3,135,262 + trpB SO3023 0.33 -2.1 3,135,262 + trpB SO3023 0.33 -1.3 3,135,262 + trpB SO3023 0.33 -2.4 3,135,264 + trpB SO3023 0.33 -1.9 3,135,270 - trpB SO3023 0.34 -2.3 3,135,270 - trpB SO3023 0.34 -1.3 3,135,270 - trpB SO3023 0.34 -2.6 3,135,270 - trpB SO3023 0.34 -0.6 3,135,272 - trpB SO3023 0.34 -1.0 3,135,272 - trpB SO3023 0.34 -0.7 3,135,398 + trpB SO3023 0.45 -3.4 3,135,624 + trpB SO3023 0.64 -0.5 3,135,651 - trpB SO3023 0.66 -3.6 3,135,659 + trpB SO3023 0.67 -3.7 3,135,775 + trpB SO3023 0.76 -2.6 3,135,775 + trpB SO3023 0.76 -1.7 3,135,783 - trpB SO3023 0.77 +1.3 3,135,805 - trpB SO3023 0.79 -0.7 3,135,805 - trpB SO3023 0.79 +0.0 3,135,933 + trpB SO3023 0.90 -0.6 3,135,941 - -1.0 3,135,943 - -1.3 3,136,061 + -0.2 3,136,092 + -1.1 3,136,150 + trpA SO3024 0.11 -1.0 3,136,160 - trpA SO3024 0.12 -1.7 3,136,183 + trpA SO3024 0.14 -0.1 3,136,191 - trpA SO3024 0.15 -1.6 3,136,191 - trpA SO3024 0.15 -0.9 3,136,218 + trpA SO3024 0.19 -2.5 3,136,242 + trpA SO3024 0.22 -2.4 3,136,339 + trpA SO3024 0.33 -0.9 3,136,390 + trpA SO3024 0.39 -0.5 3,136,390 + trpA SO3024 0.39 -2.1 3,136,409 + trpA SO3024 0.41 -1.6 3,136,417 - trpA SO3024 0.42 -2.1 3,136,417 - trpA SO3024 0.42 -2.2 3,136,530 + trpA SO3024 0.56 -2.0 3,136,532 - trpA SO3024 0.56 -1.3 3,136,532 - trpA SO3024 0.56 -3.5 3,136,538 - trpA SO3024 0.57 -0.2 3,136,610 + trpA SO3024 0.65 -2.7 3,136,618 - trpA SO3024 0.66 -1.7 3,136,722 + trpA SO3024 0.79 -1.2 3,136,757 - trpA SO3024 0.83 -1.8 3,136,757 - trpA SO3024 0.83 -1.7 3,136,987 + -2.3 3,136,987 + +1.4 3,137,004 + +0.7 3,137,004 + +0.3 3,137,012 - -0.6
Or see this region's nucleotide sequence