Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpC/F are separated by 92 nucleotides trpC/F and trpB are separated by 119 nucleotides
SO3021: trpD - anthranilate phosphoribosyltransferase (NCBI ptt file), at 3,132,201 to 3,133,244
trpD
SO3022: trpC/F - indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (NCBI ptt file), at 3,133,337 to 3,134,746
trpC/F
SO3023: trpB - tryptophan synthase, beta subunit (NCBI ptt file), at 3,134,866 to 3,136,056
trpB
Position (kb)
3133
3134
3135 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3132.412 kb on - strand, within trpD at 3132.418 kb on - strand, within trpD at 3132.419 kb on - strand, within trpD at 3132.504 kb on + strand, within trpD at 3132.596 kb on - strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.681 kb on + strand, within trpD at 3132.711 kb on + strand, within trpD at 3132.775 kb on - strand, within trpD at 3132.801 kb on + strand, within trpD at 3132.809 kb on - strand, within trpD at 3132.811 kb on + strand, within trpD at 3132.843 kb on + strand, within trpD at 3132.873 kb on + strand, within trpD at 3132.932 kb on + strand, within trpD at 3133.085 kb on - strand, within trpD at 3133.174 kb on - strand at 3133.229 kb on - strand at 3133.230 kb on - strand at 3133.268 kb on + strand at 3133.337 kb on + strand at 3133.337 kb on + strand at 3133.345 kb on - strand at 3133.406 kb on + strand at 3133.407 kb on + strand at 3133.415 kb on - strand at 3133.540 kb on - strand, within trpC/F at 3133.540 kb on - strand, within trpC/F at 3133.659 kb on + strand, within trpC/F at 3133.766 kb on + strand, within trpC/F at 3133.945 kb on + strand, within trpC/F at 3134.058 kb on + strand, within trpC/F at 3134.139 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.249 kb on + strand, within trpC/F at 3134.257 kb on - strand, within trpC/F at 3134.345 kb on + strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.365 kb on - strand, within trpC/F at 3134.399 kb on - strand, within trpC/F at 3134.402 kb on + strand, within trpC/F at 3134.431 kb on - strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.454 kb on + strand, within trpC/F at 3134.496 kb on - strand, within trpC/F at 3134.552 kb on - strand, within trpC/F at 3134.703 kb on + strand at 3134.797 kb on + strand at 3134.850 kb on + strand at 3134.880 kb on - strand at 3134.996 kb on - strand, within trpB at 3134.996 kb on - strand, within trpB at 3135.032 kb on + strand, within trpB at 3135.111 kb on - strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.139 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.205 kb on + strand, within trpB at 3135.213 kb on - strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.226 kb on + strand, within trpB at 3135.234 kb on - strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.262 kb on + strand, within trpB at 3135.264 kb on + strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.270 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.272 kb on - strand, within trpB at 3135.398 kb on + strand, within trpB at 3135.624 kb on + strand, within trpB at 3135.651 kb on - strand, within trpB at 3135.659 kb on + strand, within trpB
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 3,132,412 - trpD SO3021 0.20 -2.2 3,132,418 - trpD SO3021 0.21 -2.2 3,132,419 - trpD SO3021 0.21 -2.6 3,132,504 + trpD SO3021 0.29 -0.8 3,132,596 - trpD SO3021 0.38 +1.8 3,132,625 + trpD SO3021 0.41 -2.1 3,132,625 + trpD SO3021 0.41 -1.4 3,132,646 + trpD SO3021 0.43 +0.5 3,132,646 + trpD SO3021 0.43 -2.4 3,132,646 + trpD SO3021 0.43 -0.7 3,132,654 - trpD SO3021 0.43 +0.8 3,132,654 - trpD SO3021 0.43 -3.7 3,132,681 + trpD SO3021 0.46 -1.1 3,132,711 + trpD SO3021 0.49 -1.7 3,132,775 - trpD SO3021 0.55 +0.8 3,132,801 + trpD SO3021 0.57 -0.8 3,132,809 - trpD SO3021 0.58 -0.6 3,132,811 + trpD SO3021 0.58 -2.2 3,132,843 + trpD SO3021 0.61 -2.4 3,132,873 + trpD SO3021 0.64 +0.3 3,132,932 + trpD SO3021 0.70 -1.4 3,133,085 - trpD SO3021 0.85 -1.8 3,133,174 - -0.4 3,133,229 - -2.4 3,133,230 - -3.0 3,133,268 + -2.6 3,133,337 + -3.9 3,133,337 + -2.9 3,133,345 - -1.7 3,133,406 + -1.0 3,133,407 + -1.7 3,133,415 - -0.5 3,133,540 - trpC/F SO3022 0.14 -4.1 3,133,540 - trpC/F SO3022 0.14 -1.1 3,133,659 + trpC/F SO3022 0.23 +1.3 3,133,766 + trpC/F SO3022 0.30 -2.6 3,133,945 + trpC/F SO3022 0.43 -0.3 3,134,058 + trpC/F SO3022 0.51 -1.3 3,134,139 + trpC/F SO3022 0.57 -0.5 3,134,249 + trpC/F SO3022 0.65 -2.0 3,134,249 + trpC/F SO3022 0.65 -1.5 3,134,257 - trpC/F SO3022 0.65 -1.0 3,134,345 + trpC/F SO3022 0.71 -2.8 3,134,365 - trpC/F SO3022 0.73 -1.9 3,134,365 - trpC/F SO3022 0.73 -2.0 3,134,399 - trpC/F SO3022 0.75 -2.3 3,134,402 + trpC/F SO3022 0.76 -0.3 3,134,431 - trpC/F SO3022 0.78 -1.6 3,134,454 + trpC/F SO3022 0.79 -1.4 3,134,454 + trpC/F SO3022 0.79 -2.2 3,134,496 - trpC/F SO3022 0.82 -2.7 3,134,552 - trpC/F SO3022 0.86 +1.1 3,134,703 + -0.3 3,134,797 + -1.3 3,134,850 + -0.9 3,134,880 - -2.4 3,134,996 - trpB SO3023 0.11 -1.0 3,134,996 - trpB SO3023 0.11 -0.9 3,135,032 + trpB SO3023 0.14 -1.4 3,135,111 - trpB SO3023 0.21 -1.2 3,135,139 + trpB SO3023 0.23 +2.8 3,135,139 + trpB SO3023 0.23 -2.0 3,135,205 + trpB SO3023 0.28 -2.1 3,135,205 + trpB SO3023 0.28 -1.9 3,135,213 - trpB SO3023 0.29 -1.3 3,135,226 + trpB SO3023 0.30 -2.5 3,135,226 + trpB SO3023 0.30 -0.9 3,135,234 - trpB SO3023 0.31 +0.0 3,135,262 + trpB SO3023 0.33 -2.1 3,135,262 + trpB SO3023 0.33 -1.3 3,135,262 + trpB SO3023 0.33 -2.4 3,135,264 + trpB SO3023 0.33 -1.9 3,135,270 - trpB SO3023 0.34 -2.3 3,135,270 - trpB SO3023 0.34 -1.3 3,135,270 - trpB SO3023 0.34 -2.6 3,135,270 - trpB SO3023 0.34 -0.6 3,135,272 - trpB SO3023 0.34 -1.0 3,135,272 - trpB SO3023 0.34 -0.7 3,135,398 + trpB SO3023 0.45 -3.4 3,135,624 + trpB SO3023 0.64 -0.5 3,135,651 - trpB SO3023 0.66 -3.6 3,135,659 + trpB SO3023 0.67 -3.7
Or see this region's nucleotide sequence