Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpG are separated by 10 nucleotides trpG and trpD overlap by 1 nucleotides trpD and trpC/F are separated by 92 nucleotides
SO3019: trpE - anthranilate synthase component I (NCBI ptt file), at 3,129,858 to 3,131,582
trpE
SO3020: trpG - glutamine amido-transferase (NCBI ptt file), at 3,131,593 to 3,132,201
trpG
SO3021: trpD - anthranilate phosphoribosyltransferase (NCBI ptt file), at 3,132,201 to 3,133,244
trpD
SO3022: trpC/F - indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (NCBI ptt file), at 3,133,337 to 3,134,746
trpC/F
Position (kb)
3132
3133
3134 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3131.217 kb on + strand, within trpE at 3131.225 kb on - strand, within trpE at 3131.225 kb on - strand, within trpE at 3131.225 kb on - strand, within trpE at 3131.343 kb on - strand, within trpE at 3131.403 kb on - strand, within trpE at 3131.403 kb on - strand, within trpE at 3131.408 kb on - strand, within trpE at 3131.408 kb on - strand, within trpE at 3131.414 kb on - strand at 3131.416 kb on + strand at 3131.435 kb on - strand at 3131.511 kb on + strand at 3131.519 kb on - strand at 3131.555 kb on + strand at 3131.576 kb on + strand at 3131.584 kb on - strand at 3131.743 kb on + strand, within trpG at 3131.743 kb on + strand, within trpG at 3131.751 kb on - strand, within trpG at 3131.770 kb on - strand, within trpG at 3132.075 kb on + strand, within trpG at 3132.120 kb on - strand, within trpG at 3132.121 kb on + strand, within trpG at 3132.121 kb on + strand, within trpG at 3132.129 kb on - strand, within trpG at 3132.129 kb on - strand, within trpG at 3132.129 kb on - strand, within trpG at 3132.260 kb on - strand at 3132.281 kb on + strand at 3132.288 kb on + strand at 3132.331 kb on + strand, within trpD at 3132.331 kb on + strand, within trpD at 3132.412 kb on - strand, within trpD at 3132.418 kb on - strand, within trpD at 3132.419 kb on - strand, within trpD at 3132.504 kb on + strand, within trpD at 3132.596 kb on - strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.625 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.646 kb on + strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.654 kb on - strand, within trpD at 3132.681 kb on + strand, within trpD at 3132.711 kb on + strand, within trpD at 3132.775 kb on - strand, within trpD at 3132.801 kb on + strand, within trpD at 3132.809 kb on - strand, within trpD at 3132.811 kb on + strand, within trpD at 3132.843 kb on + strand, within trpD at 3132.873 kb on + strand, within trpD at 3132.932 kb on + strand, within trpD at 3133.085 kb on - strand, within trpD at 3133.174 kb on - strand at 3133.229 kb on - strand at 3133.230 kb on - strand at 3133.268 kb on + strand at 3133.337 kb on + strand at 3133.337 kb on + strand at 3133.345 kb on - strand at 3133.406 kb on + strand at 3133.407 kb on + strand at 3133.415 kb on - strand at 3133.540 kb on - strand, within trpC/F at 3133.540 kb on - strand, within trpC/F at 3133.659 kb on + strand, within trpC/F at 3133.766 kb on + strand, within trpC/F at 3133.945 kb on + strand, within trpC/F at 3134.058 kb on + strand, within trpC/F at 3134.139 kb on + strand, within trpC/F
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 3,131,217 + trpE SO3019 0.79 -1.6 3,131,225 - trpE SO3019 0.79 -2.5 3,131,225 - trpE SO3019 0.79 +1.3 3,131,225 - trpE SO3019 0.79 -0.4 3,131,343 - trpE SO3019 0.86 -4.2 3,131,403 - trpE SO3019 0.90 -0.6 3,131,403 - trpE SO3019 0.90 -3.3 3,131,408 - trpE SO3019 0.90 -1.8 3,131,408 - trpE SO3019 0.90 -1.3 3,131,414 - -2.4 3,131,416 + -2.1 3,131,435 - -1.9 3,131,511 + -0.7 3,131,519 - -2.9 3,131,555 + -2.0 3,131,576 + -1.9 3,131,584 - -1.6 3,131,743 + trpG SO3020 0.25 -1.8 3,131,743 + trpG SO3020 0.25 -0.6 3,131,751 - trpG SO3020 0.26 -1.2 3,131,770 - trpG SO3020 0.29 -3.3 3,132,075 + trpG SO3020 0.79 -2.3 3,132,120 - trpG SO3020 0.87 -1.4 3,132,121 + trpG SO3020 0.87 -0.2 3,132,121 + trpG SO3020 0.87 -2.2 3,132,129 - trpG SO3020 0.88 -1.3 3,132,129 - trpG SO3020 0.88 -3.0 3,132,129 - trpG SO3020 0.88 -2.3 3,132,260 - -4.0 3,132,281 + -1.8 3,132,288 + -1.7 3,132,331 + trpD SO3021 0.12 -2.1 3,132,331 + trpD SO3021 0.12 -1.6 3,132,412 - trpD SO3021 0.20 -2.2 3,132,418 - trpD SO3021 0.21 -2.2 3,132,419 - trpD SO3021 0.21 -2.6 3,132,504 + trpD SO3021 0.29 -0.8 3,132,596 - trpD SO3021 0.38 +1.8 3,132,625 + trpD SO3021 0.41 -2.1 3,132,625 + trpD SO3021 0.41 -1.4 3,132,646 + trpD SO3021 0.43 +0.5 3,132,646 + trpD SO3021 0.43 -2.4 3,132,646 + trpD SO3021 0.43 -0.7 3,132,654 - trpD SO3021 0.43 +0.8 3,132,654 - trpD SO3021 0.43 -3.7 3,132,681 + trpD SO3021 0.46 -1.1 3,132,711 + trpD SO3021 0.49 -1.7 3,132,775 - trpD SO3021 0.55 +0.8 3,132,801 + trpD SO3021 0.57 -0.8 3,132,809 - trpD SO3021 0.58 -0.6 3,132,811 + trpD SO3021 0.58 -2.2 3,132,843 + trpD SO3021 0.61 -2.4 3,132,873 + trpD SO3021 0.64 +0.3 3,132,932 + trpD SO3021 0.70 -1.4 3,133,085 - trpD SO3021 0.85 -1.8 3,133,174 - -0.4 3,133,229 - -2.4 3,133,230 - -3.0 3,133,268 + -2.6 3,133,337 + -3.9 3,133,337 + -2.9 3,133,345 - -1.7 3,133,406 + -1.0 3,133,407 + -1.7 3,133,415 - -0.5 3,133,540 - trpC/F SO3022 0.14 -4.1 3,133,540 - trpC/F SO3022 0.14 -1.1 3,133,659 + trpC/F SO3022 0.23 +1.3 3,133,766 + trpC/F SO3022 0.30 -2.6 3,133,945 + trpC/F SO3022 0.43 -0.3 3,134,058 + trpC/F SO3022 0.51 -1.3 3,134,139 + trpC/F SO3022 0.57 -0.5
Or see this region's nucleotide sequence