Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt upp and purM are separated by 306 nucleotides purM and purN overlap by 1 nucleotides purN and SO2762 are separated by 140 nucleotides
SO2759: upp - uracil phosphoribosyltransferase (NCBI ptt file), at 2,881,826 to 2,882,452
upp
SO2760: purM - phosphoribosylformylglycinamidine cyclo-ligase (NCBI ptt file), at 2,882,759 to 2,883,817
purM
SO2761: purN - phosphoribosylglycinamide formyltransferase (NCBI ptt file), at 2,883,817 to 2,884,461
purN
SO2762: SO2762 - nagD protein (NCBI ptt file), at 2,884,602 to 2,885,348
SO2762
Position (kb)
2882
2883
2884 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2881.859 kb on + strand at 2881.867 kb on - strand at 2881.867 kb on - strand at 2881.873 kb on + strand at 2881.873 kb on + strand at 2881.873 kb on + strand at 2881.873 kb on + strand at 2881.881 kb on - strand at 2881.881 kb on - strand at 2881.881 kb on - strand at 2881.881 kb on - strand at 2881.914 kb on + strand, within upp at 2881.914 kb on + strand, within upp at 2881.914 kb on + strand, within upp at 2881.969 kb on - strand, within upp at 2882.125 kb on + strand, within upp at 2882.133 kb on - strand, within upp at 2882.133 kb on - strand, within upp at 2882.133 kb on - strand, within upp at 2882.133 kb on - strand, within upp at 2882.207 kb on + strand, within upp at 2882.215 kb on - strand, within upp at 2882.250 kb on + strand, within upp at 2882.392 kb on - strand at 2882.439 kb on - strand at 2882.640 kb on - strand at 2882.787 kb on + strand at 2882.803 kb on - strand at 2882.854 kb on + strand at 2882.862 kb on - strand at 2882.883 kb on + strand, within purM at 2882.976 kb on + strand, within purM at 2883.151 kb on - strand, within purM at 2883.244 kb on - strand, within purM at 2883.244 kb on - strand, within purM at 2883.507 kb on - strand, within purM at 2883.666 kb on + strand, within purM at 2883.731 kb on - strand at 2883.962 kb on - strand, within purN at 2884.223 kb on + strand, within purN at 2884.357 kb on - strand, within purN at 2884.493 kb on - strand at 2884.583 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.630 kb on + strand at 2884.645 kb on + strand at 2884.746 kb on + strand, within SO2762 at 2884.810 kb on - strand, within SO2762
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 2,881,859 + -2.1 2,881,867 - -1.2 2,881,867 - +0.9 2,881,873 + -0.7 2,881,873 + +0.0 2,881,873 + -1.0 2,881,873 + +0.7 2,881,881 - +1.4 2,881,881 - -2.1 2,881,881 - -1.1 2,881,881 - -1.1 2,881,914 + upp SO2759 0.14 -1.0 2,881,914 + upp SO2759 0.14 -1.1 2,881,914 + upp SO2759 0.14 -2.0 2,881,969 - upp SO2759 0.23 -2.0 2,882,125 + upp SO2759 0.48 -1.4 2,882,133 - upp SO2759 0.49 -0.6 2,882,133 - upp SO2759 0.49 -1.1 2,882,133 - upp SO2759 0.49 +1.2 2,882,133 - upp SO2759 0.49 -2.8 2,882,207 + upp SO2759 0.61 -0.4 2,882,215 - upp SO2759 0.62 -0.8 2,882,250 + upp SO2759 0.68 -1.1 2,882,392 - +1.0 2,882,439 - -0.8 2,882,640 - -0.1 2,882,787 + -1.6 2,882,803 - +0.0 2,882,854 + -1.3 2,882,862 - -1.4 2,882,883 + purM SO2760 0.12 -2.3 2,882,976 + purM SO2760 0.20 -0.1 2,883,151 - purM SO2760 0.37 -0.4 2,883,244 - purM SO2760 0.46 -1.7 2,883,244 - purM SO2760 0.46 -0.4 2,883,507 - purM SO2760 0.71 -1.8 2,883,666 + purM SO2760 0.86 +1.0 2,883,731 - -0.9 2,883,962 - purN SO2761 0.22 -0.1 2,884,223 + purN SO2761 0.63 -2.9 2,884,357 - purN SO2761 0.84 -1.7 2,884,493 - -0.3 2,884,583 + +0.7 2,884,630 + -0.9 2,884,630 + +0.2 2,884,630 + +0.1 2,884,630 + +1.3 2,884,645 + -0.1 2,884,746 + SO2762 0.19 -0.4 2,884,810 - SO2762 0.28 +1.4
Or see this region's nucleotide sequence