Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and thiS are separated by 1 nucleotides thiS and thiF overlap by 4 nucleotides thiF and thiDE overlap by 4 nucleotides
SO2440: thiH - thiH protein (NCBI ptt file), at 2,553,968 to 2,555,095
thiH
SO2441: thiG - thiG protein (NCBI ptt file), at 2,555,092 to 2,555,856
thiG
SO2442: thiS - thiS protein, putative (NCBI ptt file), at 2,555,858 to 2,556,061
thiS
SO2443: thiF - thiF protein, putative (NCBI ptt file), at 2,556,058 to 2,556,960
thiF
SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537
thiDE
Position (kb)
2556
2557 Strain fitness (log2 ratio)
-2
-1
0
1 at 2555.122 kb on + strand at 2555.122 kb on + strand at 2555.147 kb on + strand at 2555.156 kb on - strand at 2555.243 kb on + strand, within thiG at 2555.243 kb on + strand, within thiG at 2555.251 kb on - strand, within thiG at 2555.410 kb on + strand, within thiG at 2555.433 kb on - strand, within thiG at 2555.489 kb on - strand, within thiG at 2555.522 kb on - strand, within thiG at 2555.563 kb on + strand, within thiG at 2555.580 kb on + strand, within thiG at 2555.855 kb on + strand at 2555.919 kb on - strand, within thiS at 2556.483 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.918 kb on - strand at 2556.971 kb on - strand at 2557.138 kb on - strand, within thiDE at 2557.138 kb on - strand, within thiDE at 2557.214 kb on - strand, within thiDE at 2557.280 kb on + strand, within thiDE at 2557.413 kb on - strand, within thiDE at 2557.483 kb on + strand, within thiDE at 2557.552 kb on + strand, within thiDE at 2557.555 kb on - strand, within thiDE at 2557.676 kb on + strand, within thiDE at 2557.690 kb on + strand, within thiDE at 2557.723 kb on + strand, within thiDE at 2557.729 kb on + strand, within thiDE at 2557.742 kb on + strand, within thiDE at 2557.760 kb on - strand, within thiDE at 2557.770 kb on + strand, within thiDE at 2557.778 kb on - strand, within thiDE at 2557.782 kb on + strand, within thiDE at 2557.790 kb on - strand, within thiDE at 2557.794 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 2,555,122 + +1.5 2,555,122 + -0.6 2,555,147 + +0.2 2,555,156 - -0.7 2,555,243 + thiG SO2441 0.20 -0.7 2,555,243 + thiG SO2441 0.20 +1.0 2,555,251 - thiG SO2441 0.21 -0.7 2,555,410 + thiG SO2441 0.42 +0.7 2,555,433 - thiG SO2441 0.45 -0.4 2,555,489 - thiG SO2441 0.52 +0.9 2,555,522 - thiG SO2441 0.56 +1.1 2,555,563 + thiG SO2441 0.62 -1.9 2,555,580 + thiG SO2441 0.64 -0.1 2,555,855 + -0.1 2,555,919 - thiS SO2442 0.30 -1.8 2,556,483 + thiF SO2443 0.47 -0.2 2,556,680 + thiF SO2443 0.69 -0.9 2,556,680 + thiF SO2443 0.69 +0.6 2,556,680 + thiF SO2443 0.69 -2.4 2,556,738 + thiF SO2443 0.75 -0.5 2,556,738 + thiF SO2443 0.75 +0.1 2,556,918 - -1.6 2,556,971 - -0.6 2,557,138 - thiDE SO2444 0.11 +0.2 2,557,138 - thiDE SO2444 0.11 +0.6 2,557,214 - thiDE SO2444 0.16 -0.4 2,557,280 + thiDE SO2444 0.20 +0.1 2,557,413 - thiDE SO2444 0.29 +0.7 2,557,483 + thiDE SO2444 0.33 +0.3 2,557,552 + thiDE SO2444 0.38 -0.8 2,557,555 - thiDE SO2444 0.38 -0.2 2,557,676 + thiDE SO2444 0.45 +0.0 2,557,690 + thiDE SO2444 0.46 -1.7 2,557,723 + thiDE SO2444 0.48 -0.6 2,557,729 + thiDE SO2444 0.49 -0.8 2,557,742 + thiDE SO2444 0.50 -0.9 2,557,760 - thiDE SO2444 0.51 +0.0 2,557,770 + thiDE SO2444 0.51 -0.4 2,557,778 - thiDE SO2444 0.52 -0.4 2,557,782 + thiDE SO2444 0.52 -0.1 2,557,790 - thiDE SO2444 0.53 -0.4 2,557,794 + thiDE SO2444 0.53 -0.7 2,557,874 + thiDE SO2444 0.58 -1.2 2,557,874 + thiDE SO2444 0.58 -1.4
Or see this region's nucleotide sequence