Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and thiS are separated by 1 nucleotides thiS and thiF overlap by 4 nucleotides thiF and thiDE overlap by 4 nucleotides
SO2440: thiH - thiH protein (NCBI ptt file), at 2,553,968 to 2,555,095
thiH
SO2441: thiG - thiG protein (NCBI ptt file), at 2,555,092 to 2,555,856
thiG
SO2442: thiS - thiS protein, putative (NCBI ptt file), at 2,555,858 to 2,556,061
thiS
SO2443: thiF - thiF protein, putative (NCBI ptt file), at 2,556,058 to 2,556,960
thiF
SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537
thiDE
Position (kb)
2555
2556
2557 Strain fitness (log2 ratio)
-2
-1
0
1 at 2554.866 kb on + strand, within thiH at 2554.866 kb on + strand, within thiH at 2554.874 kb on - strand, within thiH at 2554.874 kb on - strand, within thiH at 2554.874 kb on - strand, within thiH at 2554.914 kb on + strand, within thiH at 2555.122 kb on + strand at 2555.122 kb on + strand at 2555.147 kb on + strand at 2555.156 kb on - strand at 2555.243 kb on + strand, within thiG at 2555.243 kb on + strand, within thiG at 2555.251 kb on - strand, within thiG at 2555.410 kb on + strand, within thiG at 2555.433 kb on - strand, within thiG at 2555.489 kb on - strand, within thiG at 2555.522 kb on - strand, within thiG at 2555.563 kb on + strand, within thiG at 2555.580 kb on + strand, within thiG at 2555.855 kb on + strand at 2555.919 kb on - strand, within thiS at 2556.483 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.680 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.738 kb on + strand, within thiF at 2556.918 kb on - strand at 2556.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 2,554,866 + thiH SO2440 0.80 -0.8 2,554,866 + thiH SO2440 0.80 -1.4 2,554,874 - thiH SO2440 0.80 -1.3 2,554,874 - thiH SO2440 0.80 +0.9 2,554,874 - thiH SO2440 0.80 -0.3 2,554,914 + thiH SO2440 0.84 +0.5 2,555,122 + +1.5 2,555,122 + -0.6 2,555,147 + +0.2 2,555,156 - -0.7 2,555,243 + thiG SO2441 0.20 -0.7 2,555,243 + thiG SO2441 0.20 +1.0 2,555,251 - thiG SO2441 0.21 -0.7 2,555,410 + thiG SO2441 0.42 +0.7 2,555,433 - thiG SO2441 0.45 -0.4 2,555,489 - thiG SO2441 0.52 +0.9 2,555,522 - thiG SO2441 0.56 +1.1 2,555,563 + thiG SO2441 0.62 -1.9 2,555,580 + thiG SO2441 0.64 -0.1 2,555,855 + -0.1 2,555,919 - thiS SO2442 0.30 -1.8 2,556,483 + thiF SO2443 0.47 -0.2 2,556,680 + thiF SO2443 0.69 -0.9 2,556,680 + thiF SO2443 0.69 +0.6 2,556,680 + thiF SO2443 0.69 -2.4 2,556,738 + thiF SO2443 0.75 -0.5 2,556,738 + thiF SO2443 0.75 +0.1 2,556,918 - -1.6 2,556,971 - -0.6
Or see this region's nucleotide sequence