Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt adhB and SO1492 are separated by 412 nucleotides SO1492 and malQ are separated by 507 nucleotides
SO1490: adhB - alcohol dehydrogenase II (NCBI ptt file), at 1,555,247 to 1,556,395
adhB
SO1492: SO1492 - peptidyl-prolyl cis-trans isomerase, cyclophilin family (NCBI ptt file), at 1,556,808 to 1,557,440
SO1492
SO1493: malQ - 4-alpha-glucanotransferase (NCBI ptt file), at 1,557,948 to 1,560,257
malQ
Position (kb)
1556
1557
1558 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1555.809 kb on - strand, within adhB at 1556.008 kb on + strand, within adhB at 1556.027 kb on + strand, within adhB at 1556.075 kb on + strand, within adhB at 1556.142 kb on + strand, within adhB at 1556.142 kb on + strand, within adhB at 1556.142 kb on + strand, within adhB at 1556.142 kb on + strand, within adhB at 1556.150 kb on - strand, within adhB at 1556.150 kb on - strand, within adhB at 1556.150 kb on - strand, within adhB at 1556.150 kb on - strand, within adhB at 1556.152 kb on + strand, within adhB at 1556.171 kb on - strand, within adhB at 1556.184 kb on + strand, within adhB at 1556.187 kb on - strand, within adhB at 1556.260 kb on + strand, within adhB at 1556.263 kb on - strand, within adhB at 1556.317 kb on - strand at 1556.391 kb on + strand at 1556.560 kb on + strand at 1556.561 kb on + strand at 1556.561 kb on + strand at 1556.675 kb on + strand at 1556.683 kb on - strand at 1556.683 kb on - strand at 1556.769 kb on + strand at 1556.795 kb on + strand at 1556.831 kb on + strand at 1556.839 kb on - strand at 1556.878 kb on - strand, within SO1492 at 1556.897 kb on + strand, within SO1492 at 1556.910 kb on - strand, within SO1492 at 1556.930 kb on - strand, within SO1492 at 1556.977 kb on + strand, within SO1492 at 1557.321 kb on + strand, within SO1492 at 1557.340 kb on + strand, within SO1492 at 1557.340 kb on + strand, within SO1492 at 1557.340 kb on + strand, within SO1492 at 1557.348 kb on - strand, within SO1492 at 1557.387 kb on + strand at 1557.387 kb on + strand at 1557.465 kb on + strand at 1557.466 kb on + strand at 1557.484 kb on - strand at 1557.509 kb on - strand at 1557.640 kb on + strand at 1557.907 kb on + strand at 1557.998 kb on - strand at 1558.008 kb on + strand at 1558.013 kb on + strand at 1558.040 kb on - strand at 1558.057 kb on + strand at 1558.069 kb on + strand at 1558.098 kb on - strand at 1558.114 kb on + strand at 1558.126 kb on + strand at 1558.163 kb on + strand at 1558.163 kb on + strand at 1558.163 kb on + strand at 1558.218 kb on - strand, within malQ at 1558.273 kb on - strand, within malQ at 1558.383 kb on + strand, within malQ
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 1,555,809 - adhB SO1490 0.49 +1.0 1,556,008 + adhB SO1490 0.66 -0.4 1,556,027 + adhB SO1490 0.68 -1.8 1,556,075 + adhB SO1490 0.72 +0.2 1,556,142 + adhB SO1490 0.78 +0.1 1,556,142 + adhB SO1490 0.78 +1.9 1,556,142 + adhB SO1490 0.78 +1.4 1,556,142 + adhB SO1490 0.78 +0.6 1,556,150 - adhB SO1490 0.79 +0.8 1,556,150 - adhB SO1490 0.79 -0.1 1,556,150 - adhB SO1490 0.79 +1.1 1,556,150 - adhB SO1490 0.79 -1.6 1,556,152 + adhB SO1490 0.79 +0.1 1,556,171 - adhB SO1490 0.80 +2.4 1,556,184 + adhB SO1490 0.82 +0.4 1,556,187 - adhB SO1490 0.82 +1.5 1,556,260 + adhB SO1490 0.88 +0.6 1,556,263 - adhB SO1490 0.88 +0.5 1,556,317 - +0.0 1,556,391 + +0.3 1,556,560 + -1.5 1,556,561 + -0.1 1,556,561 + +0.9 1,556,675 + -0.8 1,556,683 - -1.7 1,556,683 - +0.5 1,556,769 + +1.1 1,556,795 + +1.0 1,556,831 + -0.1 1,556,839 - +0.9 1,556,878 - SO1492 0.11 -1.9 1,556,897 + SO1492 0.14 +0.1 1,556,910 - SO1492 0.16 +0.1 1,556,930 - SO1492 0.19 +0.4 1,556,977 + SO1492 0.27 +0.5 1,557,321 + SO1492 0.81 -0.5 1,557,340 + SO1492 0.84 +3.0 1,557,340 + SO1492 0.84 +0.8 1,557,340 + SO1492 0.84 -0.8 1,557,348 - SO1492 0.85 -0.5 1,557,387 + -3.2 1,557,387 + +1.5 1,557,465 + -0.4 1,557,466 + -0.9 1,557,484 - +0.9 1,557,509 - +0.7 1,557,640 + -1.4 1,557,907 + +3.3 1,557,998 - +1.0 1,558,008 + +1.2 1,558,013 + -1.0 1,558,040 - +0.8 1,558,057 + -0.2 1,558,069 + -0.9 1,558,098 - -0.7 1,558,114 + +0.1 1,558,126 + -0.6 1,558,163 + -1.9 1,558,163 + -0.9 1,558,163 + -0.9 1,558,218 - malQ SO1493 0.12 +0.7 1,558,273 - malQ SO1493 0.14 -0.3 1,558,383 + malQ SO1493 0.19 +1.3
Or see this region's nucleotide sequence