Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD-1 and secF-1 are separated by 2 nucleotides secF-1 and SO1195 are separated by 56 nucleotides SO1195 and rrmJ are separated by 90 nucleotides
SO1193: secD-1 - protein-export membrane protein SecD (NCBI ptt file), at 1,235,992 to 1,237,848
secD-1
SO1194: secF-1 - protein-export membrane protein SecF (NCBI ptt file), at 1,237,851 to 1,238,735
secF-1
SO1195: SO1195 - conserved hypothetical protein TIGR00253 (NCBI ptt file), at 1,238,792 to 1,239,091
SO1195
SO1196: rrmJ - ribosomal RNA large subunit methyltransferase J (NCBI ptt file), at 1,239,182 to 1,239,811
rrmJ
Position (kb)
1237
1238
1239 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1236.898 kb on + strand, within secD-1 at 1236.918 kb on - strand, within secD-1 at 1236.986 kb on - strand, within secD-1 at 1237.157 kb on - strand, within secD-1 at 1237.157 kb on - strand, within secD-1 at 1237.325 kb on + strand, within secD-1 at 1237.380 kb on - strand, within secD-1 at 1237.381 kb on - strand, within secD-1 at 1237.385 kb on + strand, within secD-1 at 1237.385 kb on + strand, within secD-1 at 1237.393 kb on - strand, within secD-1 at 1237.393 kb on - strand, within secD-1 at 1237.393 kb on - strand, within secD-1 at 1237.398 kb on + strand, within secD-1 at 1237.501 kb on + strand, within secD-1 at 1237.501 kb on + strand, within secD-1 at 1237.504 kb on - strand, within secD-1 at 1237.509 kb on - strand, within secD-1 at 1237.509 kb on - strand, within secD-1 at 1237.533 kb on - strand, within secD-1 at 1237.539 kb on - strand, within secD-1 at 1237.564 kb on - strand, within secD-1 at 1237.610 kb on + strand, within secD-1 at 1237.633 kb on + strand, within secD-1 at 1237.735 kb on + strand at 1237.755 kb on - strand at 1237.755 kb on - strand at 1237.849 kb on - strand at 1237.858 kb on + strand at 1237.858 kb on + strand at 1237.906 kb on + strand at 1238.143 kb on + strand, within secF-1 at 1238.151 kb on - strand, within secF-1 at 1238.151 kb on - strand, within secF-1 at 1238.199 kb on - strand, within secF-1 at 1238.227 kb on + strand, within secF-1 at 1238.237 kb on - strand, within secF-1 at 1238.245 kb on - strand, within secF-1 at 1238.402 kb on - strand, within secF-1 at 1238.550 kb on + strand, within secF-1 at 1238.603 kb on - strand, within secF-1 at 1238.611 kb on - strand, within secF-1 at 1238.976 kb on + strand, within SO1195 at 1239.139 kb on - strand at 1239.139 kb on - strand at 1239.141 kb on + strand at 1239.343 kb on - strand, within rrmJ at 1239.660 kb on + strand, within rrmJ
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 1,236,898 + secD-1 SO1193 0.49 +2.8 1,236,918 - secD-1 SO1193 0.50 -1.1 1,236,986 - secD-1 SO1193 0.54 +0.2 1,237,157 - secD-1 SO1193 0.63 +3.3 1,237,157 - secD-1 SO1193 0.63 +1.2 1,237,325 + secD-1 SO1193 0.72 +0.3 1,237,380 - secD-1 SO1193 0.75 -1.9 1,237,381 - secD-1 SO1193 0.75 +0.0 1,237,385 + secD-1 SO1193 0.75 -1.0 1,237,385 + secD-1 SO1193 0.75 -0.8 1,237,393 - secD-1 SO1193 0.75 +1.8 1,237,393 - secD-1 SO1193 0.75 -0.0 1,237,393 - secD-1 SO1193 0.75 +1.1 1,237,398 + secD-1 SO1193 0.76 -0.3 1,237,501 + secD-1 SO1193 0.81 -0.4 1,237,501 + secD-1 SO1193 0.81 +1.2 1,237,504 - secD-1 SO1193 0.81 +1.8 1,237,509 - secD-1 SO1193 0.82 +0.0 1,237,509 - secD-1 SO1193 0.82 -1.7 1,237,533 - secD-1 SO1193 0.83 +0.5 1,237,539 - secD-1 SO1193 0.83 -1.0 1,237,564 - secD-1 SO1193 0.85 -3.1 1,237,610 + secD-1 SO1193 0.87 -0.7 1,237,633 + secD-1 SO1193 0.88 -1.3 1,237,735 + -0.7 1,237,755 - -0.6 1,237,755 - +0.7 1,237,849 - -2.1 1,237,858 + +0.5 1,237,858 + -0.8 1,237,906 + -1.3 1,238,143 + secF-1 SO1194 0.33 +0.1 1,238,151 - secF-1 SO1194 0.34 +0.7 1,238,151 - secF-1 SO1194 0.34 -0.0 1,238,199 - secF-1 SO1194 0.39 -1.3 1,238,227 + secF-1 SO1194 0.42 +2.0 1,238,237 - secF-1 SO1194 0.44 -0.8 1,238,245 - secF-1 SO1194 0.45 +0.4 1,238,402 - secF-1 SO1194 0.62 -0.9 1,238,550 + secF-1 SO1194 0.79 -0.3 1,238,603 - secF-1 SO1194 0.85 -0.9 1,238,611 - secF-1 SO1194 0.86 +1.2 1,238,976 + SO1195 0.61 -0.5 1,239,139 - -2.3 1,239,139 - -0.5 1,239,141 + -0.7 1,239,343 - rrmJ SO1196 0.26 -2.6 1,239,660 + rrmJ SO1196 0.76 +0.2
Or see this region's nucleotide sequence