Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobT and cobS are separated by 106 nucleotides cobS and cobU overlap by 4 nucleotides cobU and cobQ are separated by 211 nucleotides
SO1035: cobT - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (NCBI ptt file), at 1,074,417 to 1,075,469
cobT
SO1036: cobS - cobalamin 5'-phosphate synthase (NCBI ptt file), at 1,075,576 to 1,076,364
cobS
SO1037: cobU - cobinamide kinase/cobinamide phosphate guanylyltransferase (NCBI ptt file), at 1,076,361 to 1,076,966
cobU
SO1038: cobQ - cobyric acid synthase CobQ (NCBI ptt file), at 1,077,178 to 1,078,710
cobQ
Position (kb)
1075
1076
1077 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1074.581 kb on - strand, within cobT at 1074.744 kb on - strand, within cobT at 1074.759 kb on + strand, within cobT at 1074.774 kb on - strand, within cobT at 1075.054 kb on + strand, within cobT at 1075.159 kb on + strand, within cobT at 1075.167 kb on - strand, within cobT at 1075.257 kb on - strand, within cobT at 1075.328 kb on + strand, within cobT at 1075.362 kb on + strand, within cobT at 1075.459 kb on - strand at 1075.461 kb on + strand at 1075.464 kb on + strand at 1075.469 kb on - strand at 1075.694 kb on - strand, within cobS at 1075.742 kb on - strand, within cobS at 1075.849 kb on + strand, within cobS at 1075.884 kb on - strand, within cobS at 1075.885 kb on + strand, within cobS at 1076.038 kb on + strand, within cobS at 1076.071 kb on - strand, within cobS at 1076.086 kb on - strand, within cobS at 1076.168 kb on + strand, within cobS at 1076.230 kb on + strand, within cobS at 1076.248 kb on - strand, within cobS at 1076.330 kb on + strand at 1076.373 kb on + strand at 1076.463 kb on - strand, within cobU at 1076.626 kb on + strand, within cobU at 1076.626 kb on + strand, within cobU at 1076.626 kb on + strand, within cobU at 1076.634 kb on - strand, within cobU at 1076.754 kb on - strand, within cobU at 1076.826 kb on + strand, within cobU at 1076.898 kb on + strand, within cobU at 1076.921 kb on + strand at 1076.961 kb on + strand at 1077.070 kb on + strand at 1077.115 kb on - strand at 1077.142 kb on - strand at 1077.194 kb on + strand at 1077.194 kb on + strand at 1077.194 kb on + strand at 1077.194 kb on + strand at 1077.194 kb on - strand at 1077.202 kb on - strand at 1077.202 kb on - strand at 1077.302 kb on - strand at 1077.318 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 1,074,581 - cobT SO1035 0.16 -0.6 1,074,744 - cobT SO1035 0.31 +0.8 1,074,759 + cobT SO1035 0.32 -1.6 1,074,774 - cobT SO1035 0.34 +0.5 1,075,054 + cobT SO1035 0.60 +0.9 1,075,159 + cobT SO1035 0.70 +1.7 1,075,167 - cobT SO1035 0.71 +0.7 1,075,257 - cobT SO1035 0.80 -1.8 1,075,328 + cobT SO1035 0.87 -0.3 1,075,362 + cobT SO1035 0.90 -0.7 1,075,459 - -1.1 1,075,461 + -0.8 1,075,464 + -1.5 1,075,469 - -4.2 1,075,694 - cobS SO1036 0.15 -3.2 1,075,742 - cobS SO1036 0.21 -1.7 1,075,849 + cobS SO1036 0.35 -2.0 1,075,884 - cobS SO1036 0.39 -1.2 1,075,885 + cobS SO1036 0.39 -1.9 1,076,038 + cobS SO1036 0.59 -2.2 1,076,071 - cobS SO1036 0.63 +0.0 1,076,086 - cobS SO1036 0.65 -1.0 1,076,168 + cobS SO1036 0.75 -1.4 1,076,230 + cobS SO1036 0.83 -1.3 1,076,248 - cobS SO1036 0.85 -1.6 1,076,330 + -0.4 1,076,373 + -0.7 1,076,463 - cobU SO1037 0.17 -1.2 1,076,626 + cobU SO1037 0.44 +0.5 1,076,626 + cobU SO1037 0.44 -0.4 1,076,626 + cobU SO1037 0.44 +1.1 1,076,634 - cobU SO1037 0.45 -0.7 1,076,754 - cobU SO1037 0.65 +0.0 1,076,826 + cobU SO1037 0.77 +0.9 1,076,898 + cobU SO1037 0.89 +1.0 1,076,921 + -0.1 1,076,961 + -0.0 1,077,070 + +0.3 1,077,115 - -2.1 1,077,142 - +0.2 1,077,194 + +0.5 1,077,194 + +0.2 1,077,194 + +1.5 1,077,194 + +0.8 1,077,194 - -0.4 1,077,202 - -0.2 1,077,202 - -0.2 1,077,302 - +1.1 1,077,318 - +1.5
Or see this region's nucleotide sequence