Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspC-2 and SO2407 are separated by 401 nucleotides SO2407 and SO2408 are separated by 22 nucleotides SO2408 and SO2409 overlap by 4 nucleotides
SO2406: aspC-2 - aspartate aminotransferase (NCBI ptt file), at 2,509,451 to 2,510,641
aspC-2
SO2407: SO2407 - conserved hypothetical protein (NCBI ptt file), at 2,511,043 to 2,512,602
SO2407
SO2408: SO2408 - radical activating enzyme (NCBI ptt file), at 2,512,625 to 2,513,485
SO2408
SO2409: SO2409 - transcriptional regulator, LysR family (NCBI ptt file), at 2,513,482 to 2,514,402
SO2409
Position (kb)
2511
2512
2513 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2510.064 kb on + strand, within aspC-2 at 2510.178 kb on - strand, within aspC-2 at 2510.275 kb on + strand, within aspC-2 at 2510.275 kb on + strand, within aspC-2 at 2510.275 kb on + strand, within aspC-2 at 2510.301 kb on - strand, within aspC-2 at 2510.370 kb on + strand, within aspC-2 at 2510.378 kb on - strand, within aspC-2 at 2510.388 kb on - strand, within aspC-2 at 2510.562 kb on + strand at 2510.572 kb on + strand at 2510.580 kb on - strand at 2510.815 kb on + strand at 2511.087 kb on + strand at 2511.095 kb on - strand at 2511.136 kb on + strand at 2511.212 kb on + strand, within SO2407 at 2511.212 kb on + strand, within SO2407 at 2511.220 kb on - strand, within SO2407 at 2512.099 kb on - strand, within SO2407 at 2512.233 kb on + strand, within SO2407 at 2512.253 kb on - strand, within SO2407 at 2512.298 kb on - strand, within SO2407 at 2512.368 kb on + strand, within SO2407 at 2512.526 kb on - strand at 2512.824 kb on - strand, within SO2408 at 2512.852 kb on + strand, within SO2408 at 2512.898 kb on - strand, within SO2408 at 2512.962 kb on + strand, within SO2408 at 2512.967 kb on + strand, within SO2408 at 2513.135 kb on - strand, within SO2408 at 2513.135 kb on - strand, within SO2408 at 2513.170 kb on - strand, within SO2408 at 2513.295 kb on - strand, within SO2408 at 2513.441 kb on - strand at 2513.441 kb on - strand at 2513.489 kb on + strand at 2513.532 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 2,510,064 + aspC-2 SO2406 0.51 -0.6 2,510,178 - aspC-2 SO2406 0.61 -0.6 2,510,275 + aspC-2 SO2406 0.69 -2.3 2,510,275 + aspC-2 SO2406 0.69 -0.2 2,510,275 + aspC-2 SO2406 0.69 -1.5 2,510,301 - aspC-2 SO2406 0.71 +0.9 2,510,370 + aspC-2 SO2406 0.77 +0.8 2,510,378 - aspC-2 SO2406 0.78 -0.3 2,510,388 - aspC-2 SO2406 0.79 +0.0 2,510,562 + -2.4 2,510,572 + -1.0 2,510,580 - -0.1 2,510,815 + -0.6 2,511,087 + +1.8 2,511,095 - -1.0 2,511,136 + -0.4 2,511,212 + SO2407 0.11 -0.2 2,511,212 + SO2407 0.11 -1.4 2,511,220 - SO2407 0.11 -1.7 2,512,099 - SO2407 0.68 -1.9 2,512,233 + SO2407 0.76 +0.5 2,512,253 - SO2407 0.78 +2.2 2,512,298 - SO2407 0.80 -1.3 2,512,368 + SO2407 0.85 -1.2 2,512,526 - -0.1 2,512,824 - SO2408 0.23 +0.7 2,512,852 + SO2408 0.26 -0.5 2,512,898 - SO2408 0.32 -2.3 2,512,962 + SO2408 0.39 +0.9 2,512,967 + SO2408 0.40 +0.8 2,513,135 - SO2408 0.59 +1.4 2,513,135 - SO2408 0.59 -0.8 2,513,170 - SO2408 0.63 +1.8 2,513,295 - SO2408 0.78 -0.1 2,513,441 - -0.4 2,513,441 - +0.0 2,513,489 + +2.2 2,513,532 + -1.0
Or see this region's nucleotide sequence