Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1679 and SO1680 are separated by 52 nucleotides SO1680 and SO1681 overlap by 1 nucleotides SO1681 and mmsB are separated by 83 nucleotides
SO1679: SO1679 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 1,766,172 to 1,767,329
SO1679
SO1680: SO1680 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,767,382 to 1,768,155
SO1680
SO1681: SO1681 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,768,155 to 1,769,306
SO1681
SO1682: mmsB - 3-hydroxyisobutyrate dehydrogenase (NCBI ptt file), at 1,769,390 to 1,770,292
mmsB
Position (kb)
1768
1769
1770 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1767.267 kb on - strand at 1767.350 kb on + strand at 1767.358 kb on - strand at 1767.370 kb on - strand at 1767.461 kb on + strand, within SO1680 at 1767.475 kb on - strand, within SO1680 at 1767.572 kb on + strand, within SO1680 at 1767.600 kb on + strand, within SO1680 at 1767.600 kb on + strand, within SO1680 at 1767.600 kb on + strand, within SO1680 at 1767.608 kb on - strand, within SO1680 at 1767.627 kb on - strand, within SO1680 at 1767.640 kb on + strand, within SO1680 at 1767.655 kb on - strand, within SO1680 at 1767.715 kb on - strand, within SO1680 at 1767.718 kb on + strand, within SO1680 at 1767.718 kb on + strand, within SO1680 at 1767.723 kb on + strand, within SO1680 at 1767.792 kb on + strand, within SO1680 at 1767.795 kb on - strand, within SO1680 at 1767.837 kb on + strand, within SO1680 at 1767.845 kb on - strand, within SO1680 at 1767.845 kb on - strand, within SO1680 at 1768.106 kb on - strand at 1768.128 kb on + strand at 1768.184 kb on + strand at 1768.280 kb on - strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.526 kb on + strand, within SO1681 at 1768.650 kb on + strand, within SO1681 at 1768.717 kb on + strand, within SO1681 at 1768.752 kb on - strand, within SO1681 at 1768.880 kb on - strand, within SO1681 at 1768.921 kb on + strand, within SO1681 at 1769.040 kb on + strand, within SO1681 at 1769.040 kb on + strand, within SO1681 at 1769.080 kb on - strand, within SO1681 at 1769.090 kb on + strand, within SO1681 at 1769.240 kb on - strand at 1769.242 kb on + strand at 1769.250 kb on - strand at 1769.276 kb on - strand at 1769.309 kb on - strand at 1769.340 kb on - strand at 1769.375 kb on - strand at 1769.392 kb on + strand at 1769.402 kb on - strand at 1769.438 kb on - strand at 1769.707 kb on + strand, within mmsB at 1769.726 kb on + strand, within mmsB at 1769.783 kb on + strand, within mmsB at 1769.835 kb on - strand, within mmsB at 1769.844 kb on - strand, within mmsB at 1769.895 kb on - strand, within mmsB at 1769.960 kb on - strand, within mmsB at 1769.981 kb on - strand, within mmsB at 1770.060 kb on - strand, within mmsB at 1770.106 kb on - strand, within mmsB
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 1,767,267 - +1.4 1,767,350 + +0.5 1,767,358 - +2.8 1,767,370 - +2.9 1,767,461 + SO1680 0.10 +1.6 1,767,475 - SO1680 0.12 +3.2 1,767,572 + SO1680 0.25 +1.8 1,767,600 + SO1680 0.28 +1.8 1,767,600 + SO1680 0.28 +1.4 1,767,600 + SO1680 0.28 +0.6 1,767,608 - SO1680 0.29 +2.8 1,767,627 - SO1680 0.32 +2.8 1,767,640 + SO1680 0.33 +1.9 1,767,655 - SO1680 0.35 +2.4 1,767,715 - SO1680 0.43 +2.2 1,767,718 + SO1680 0.43 -1.8 1,767,718 + SO1680 0.43 +2.3 1,767,723 + SO1680 0.44 +1.9 1,767,792 + SO1680 0.53 +1.2 1,767,795 - SO1680 0.53 +3.2 1,767,837 + SO1680 0.59 -1.5 1,767,845 - SO1680 0.60 +2.1 1,767,845 - SO1680 0.60 +2.5 1,768,106 - +3.0 1,768,128 + +0.6 1,768,184 + +3.0 1,768,280 - SO1681 0.11 +4.3 1,768,526 + SO1681 0.32 +3.3 1,768,526 + SO1681 0.32 +3.4 1,768,526 + SO1681 0.32 +3.6 1,768,526 + SO1681 0.32 +3.2 1,768,650 + SO1681 0.43 +3.8 1,768,717 + SO1681 0.49 +3.0 1,768,752 - SO1681 0.52 +1.5 1,768,880 - SO1681 0.63 +2.5 1,768,921 + SO1681 0.66 +2.5 1,769,040 + SO1681 0.77 +3.0 1,769,040 + SO1681 0.77 +3.1 1,769,080 - SO1681 0.80 +3.0 1,769,090 + SO1681 0.81 +4.7 1,769,240 - +2.7 1,769,242 + +2.3 1,769,250 - +3.0 1,769,276 - +2.0 1,769,309 - +2.0 1,769,340 - -1.3 1,769,375 - +2.3 1,769,392 + +0.7 1,769,402 - -0.9 1,769,438 - -0.1 1,769,707 + mmsB SO1682 0.35 -2.2 1,769,726 + mmsB SO1682 0.37 +0.8 1,769,783 + mmsB SO1682 0.44 +0.5 1,769,835 - mmsB SO1682 0.49 +0.1 1,769,844 - mmsB SO1682 0.50 +1.2 1,769,895 - mmsB SO1682 0.56 +1.1 1,769,960 - mmsB SO1682 0.63 +0.8 1,769,981 - mmsB SO1682 0.65 -0.4 1,770,060 - mmsB SO1682 0.74 +2.8 1,770,106 - mmsB SO1682 0.79 +1.2
Or see this region's nucleotide sequence