Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrdA and SO1169 are separated by 63 nucleotides SO1169 and SO1170 overlap by 1 nucleotides SO1170 and nad are separated by 107 nucleotides nad and holA are separated by 12 nucleotides
SO1168: mrdA - penicillin-binding protein 2 (NCBI ptt file), at 1,210,703 to 1,212,559
mrdA
SO1169: SO1169 - conserved hypothetical protein TIGR00246 (NCBI ptt file), at 1,212,623 to 1,213,093
SO1169
SO1170: SO1170 - iojap domain protein (NCBI ptt file), at 1,213,093 to 1,213,422
SO1170
SO1171: nad - nicotinate (nicotinamide) nucleotide adenylyltransferase (NCBI ptt file), at 1,213,530 to 1,214,168
nad
SO1172: holA - DNA polymerase III, delta subunit (NCBI ptt file), at 1,214,181 to 1,215,212
holA
Position (kb)
1213
1214 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1212.618 kb on - strand at 1212.676 kb on - strand, within SO1169 at 1212.676 kb on - strand, within SO1169 at 1212.720 kb on - strand, within SO1169 at 1212.721 kb on - strand, within SO1169 at 1212.772 kb on - strand, within SO1169 at 1212.849 kb on - strand, within SO1169 at 1212.879 kb on - strand, within SO1169 at 1212.902 kb on - strand, within SO1169 at 1213.012 kb on - strand, within SO1169 at 1213.055 kb on - strand at 1213.149 kb on - strand, within SO1170 at 1213.434 kb on - strand at 1213.457 kb on + strand at 1213.466 kb on + strand at 1213.474 kb on - strand at 1214.186 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 1,212,618 - +0.7 1,212,676 - SO1169 0.11 -1.0 1,212,676 - SO1169 0.11 +0.8 1,212,720 - SO1169 0.21 -2.3 1,212,721 - SO1169 0.21 +1.2 1,212,772 - SO1169 0.32 -1.0 1,212,849 - SO1169 0.48 -2.4 1,212,879 - SO1169 0.54 -2.0 1,212,902 - SO1169 0.59 -3.5 1,213,012 - SO1169 0.83 -1.4 1,213,055 - -1.4 1,213,149 - SO1170 0.17 -3.1 1,213,434 - -2.1 1,213,457 + -0.0 1,213,466 + -1.9 1,213,474 - -2.4 1,214,186 - +0.5
Or see this region's nucleotide sequence