Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fusA-2 and SO0843 are separated by 213 nucleotides SO0843 and SO0844 are separated by 94 nucleotides SO0844 and napB are separated by 175 nucleotides napB and napH overlap by 4 nucleotides
SO0842: fusA-2 - translation elongation factor G (NCBI ptt file), at 863,203 to 865,296
fusA-2
SO0843: SO0843 - transcriptional regulator, LysR family (NCBI ptt file), at 865,510 to 866,391
SO0843
SO0844: SO0844 - hypothetical protein (NCBI ptt file), at 866,486 to 866,638
SO0844
SO0845: napB - cytochrome c-type protein NapB (NCBI ptt file), at 866,814 to 867,245
napB
SO0846: napH - iron-sulfur cluster-binding protein napH (NCBI ptt file), at 867,242 to 868,201
napH
Position (kb) 865
866
867 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 865.269 kb on + strand at 865.277 kb on - strand at 865.277 kb on - strand at 865.443 kb on + strand at 865.561 kb on + strand at 865.574 kb on + strand at 865.616 kb on + strand, within SO0843 at 865.624 kb on - strand, within SO0843 at 865.714 kb on + strand, within SO0843 at 865.749 kb on + strand, within SO0843 at 865.803 kb on - strand, within SO0843 at 865.854 kb on - strand, within SO0843 at 865.937 kb on + strand, within SO0843 at 865.937 kb on + strand, within SO0843 at 865.937 kb on + strand, within SO0843 at 865.937 kb on + strand, within SO0843 at 865.937 kb on + strand, within SO0843 at 865.945 kb on - strand, within SO0843 at 865.945 kb on - strand, within SO0843 at 865.945 kb on - strand, within SO0843 at 866.036 kb on + strand, within SO0843 at 866.080 kb on + strand, within SO0843 at 866.103 kb on + strand, within SO0843 at 866.111 kb on - strand, within SO0843 at 866.386 kb on + strand at 866.469 kb on + strand at 866.578 kb on - strand, within SO0844 at 866.683 kb on - strand at 866.755 kb on + strand at 866.774 kb on - strand at 866.786 kb on - strand at 866.869 kb on + strand, within napB at 866.950 kb on + strand, within napB at 866.958 kb on - strand, within napB at 866.958 kb on - strand, within napB at 866.958 kb on - strand at 866.965 kb on + strand, within napB at 866.965 kb on + strand, within napB at 866.973 kb on - strand, within napB at 867.066 kb on + strand, within napB at 867.132 kb on + strand, within napB at 867.163 kb on + strand, within napB at 867.188 kb on + strand, within napB at 867.297 kb on + strand at 867.390 kb on - strand, within napH at 867.391 kb on + strand, within napH
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 865,269 + -0.8 865,277 - +2.4 865,277 - +1.4 865,443 + +1.1 865,561 + -0.2 865,574 + -0.4 865,616 + SO0843 0.12 +1.0 865,624 - SO0843 0.13 +0.6 865,714 + SO0843 0.23 +2.4 865,749 + SO0843 0.27 +1.9 865,803 - SO0843 0.33 +1.8 865,854 - SO0843 0.39 -0.7 865,937 + SO0843 0.48 +0.4 865,937 + SO0843 0.48 -2.8 865,937 + SO0843 0.48 +0.0 865,937 + SO0843 0.48 -1.6 865,937 + SO0843 0.48 +1.1 865,945 - SO0843 0.49 +0.1 865,945 - SO0843 0.49 +0.0 865,945 - SO0843 0.49 -2.1 866,036 + SO0843 0.60 +0.2 866,080 + SO0843 0.65 +0.0 866,103 + SO0843 0.67 -1.1 866,111 - SO0843 0.68 +1.2 866,386 + +0.4 866,469 + +2.6 866,578 - SO0844 0.60 -0.8 866,683 - -0.6 866,755 + +0.9 866,774 - -0.3 866,786 - -0.8 866,869 + napB SO0845 0.13 +1.1 866,950 + napB SO0845 0.31 -1.3 866,958 - napB SO0845 0.33 +0.2 866,958 - napB SO0845 0.33 -0.8 866,958 - +0.9 866,965 + napB SO0845 0.35 +1.8 866,965 + napB SO0845 0.35 +0.6 866,973 - napB SO0845 0.37 +0.4 867,066 + napB SO0845 0.58 -1.0 867,132 + napB SO0845 0.74 +0.5 867,163 + napB SO0845 0.81 -0.3 867,188 + napB SO0845 0.87 -0.6 867,297 + +0.9 867,390 - napH SO0846 0.15 +0.0 867,391 + napH SO0846 0.16 +0.9
Or see this region's nucleotide sequence