Experiment: MoYLS4 with Molybdate 100uM and Tungstate 2mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DORF2116 and DVUA0118 overlap by 22 nucleotides DVUA0118 and sctN are separated by 327 nucleotides sctN and DVUA0119.1 are separated by 3 nucleotides DVUA0119.1 and DVUA0120 are separated by 12 nucleotides
DORF2116: DORF2116 - SEED: FIG00606188: hypothetical protein, at 155,792 to 156,715
DORF2116
DVUA0118: DVUA0118 - type III secretion protein, YopL family (TIGR), at 156,694 to 157,311
DVUA0118
DVUA0119: sctN - type III secretion system ATPase (TIGR), at 157,639 to 158,955
sctN
DVUA0119.1: DVUA0119.1 - SEED: Type III secretion spans bacterial envelope protein (YscO), at 158,959 to 159,453
DVUA0119.1
DVUA0120: DVUA0120 - type III secretion protein, putative (TIGR), at 159,466 to 160,158
DVUA0120
Position (kb) 157
158
159 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 156.713 kb on - strand at 156.887 kb on + strand, within DVUA0118 at 156.895 kb on - strand, within DVUA0118 at 156.922 kb on + strand, within DVUA0118 at 156.934 kb on + strand, within DVUA0118 at 156.949 kb on - strand, within DVUA0118 at 157.445 kb on - strand at 157.459 kb on + strand at 157.474 kb on + strand at 157.482 kb on - strand at 157.482 kb on - strand at 157.562 kb on - strand at 157.607 kb on + strand at 157.665 kb on - strand at 157.840 kb on + strand, within sctN at 157.863 kb on + strand, within sctN at 157.870 kb on + strand, within sctN at 157.896 kb on + strand, within sctN at 157.904 kb on - strand, within sctN at 158.034 kb on + strand, within sctN at 158.042 kb on - strand, within sctN at 158.066 kb on + strand, within sctN at 158.092 kb on + strand, within sctN at 158.109 kb on - strand, within sctN at 158.119 kb on + strand, within sctN at 158.242 kb on - strand, within sctN at 158.242 kb on - strand, within sctN at 158.270 kb on + strand, within sctN at 158.304 kb on + strand, within sctN at 158.334 kb on + strand, within sctN at 158.432 kb on - strand, within sctN at 158.556 kb on - strand, within sctN at 158.562 kb on + strand, within sctN at 158.562 kb on + strand, within sctN at 158.568 kb on - strand, within sctN at 158.585 kb on - strand, within sctN at 158.635 kb on + strand, within sctN at 158.705 kb on - strand, within sctN at 158.737 kb on + strand, within sctN at 158.737 kb on + strand, within sctN at 158.739 kb on + strand, within sctN at 158.745 kb on - strand, within sctN at 158.879 kb on + strand at 158.932 kb on + strand at 158.937 kb on + strand at 158.937 kb on + strand at 158.937 kb on + strand at 158.937 kb on + strand at 158.937 kb on + strand at 158.940 kb on - strand at 158.945 kb on - strand at 158.945 kb on - strand at 158.950 kb on + strand at 158.950 kb on + strand at 158.958 kb on - strand at 158.994 kb on - strand at 159.039 kb on - strand, within DVUA0119.1 at 159.046 kb on - strand, within DVUA0119.1 at 159.065 kb on - strand, within DVUA0119.1 at 159.162 kb on + strand, within DVUA0119.1 at 159.163 kb on + strand, within DVUA0119.1 at 159.163 kb on + strand, within DVUA0119.1 at 159.170 kb on - strand, within DVUA0119.1 at 159.227 kb on - strand, within DVUA0119.1 at 159.373 kb on - strand, within DVUA0119.1 at 159.414 kb on + strand at 159.422 kb on - strand at 159.440 kb on + strand at 159.521 kb on + strand at 159.589 kb on - strand, within DVUA0120 at 159.626 kb on + strand, within DVUA0120 at 159.752 kb on + strand, within DVUA0120 at 159.767 kb on + strand, within DVUA0120 at 159.920 kb on - strand, within DVUA0120 at 159.943 kb on + strand, within DVUA0120
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Molybdate 100uM and Tungstate 2mM remove 156,713 - -1.3 156,887 + DVUA0118 0.31 -0.8 156,895 - DVUA0118 0.33 -0.5 156,922 + DVUA0118 0.37 -0.6 156,934 + DVUA0118 0.39 +0.5 156,949 - DVUA0118 0.41 -2.3 157,445 - +0.7 157,459 + +1.1 157,474 + -0.8 157,482 - -1.4 157,482 - -0.5 157,562 - -1.6 157,607 + -1.0 157,665 - -1.7 157,840 + sctN DVUA0119 0.15 -0.6 157,863 + sctN DVUA0119 0.17 +1.0 157,870 + sctN DVUA0119 0.18 -1.1 157,896 + sctN DVUA0119 0.20 +0.2 157,904 - sctN DVUA0119 0.20 -0.6 158,034 + sctN DVUA0119 0.30 +1.6 158,042 - sctN DVUA0119 0.31 +0.0 158,066 + sctN DVUA0119 0.32 -1.2 158,092 + sctN DVUA0119 0.34 -0.9 158,109 - sctN DVUA0119 0.36 -1.4 158,119 + sctN DVUA0119 0.36 +0.1 158,242 - sctN DVUA0119 0.46 +2.7 158,242 - sctN DVUA0119 0.46 -2.4 158,270 + sctN DVUA0119 0.48 -0.3 158,304 + sctN DVUA0119 0.50 +0.1 158,334 + sctN DVUA0119 0.53 -1.0 158,432 - sctN DVUA0119 0.60 +1.4 158,556 - sctN DVUA0119 0.70 -0.6 158,562 + sctN DVUA0119 0.70 +0.3 158,562 + sctN DVUA0119 0.70 -0.3 158,568 - sctN DVUA0119 0.71 -0.8 158,585 - sctN DVUA0119 0.72 +1.0 158,635 + sctN DVUA0119 0.76 +1.7 158,705 - sctN DVUA0119 0.81 +0.4 158,737 + sctN DVUA0119 0.83 -2.0 158,737 + sctN DVUA0119 0.83 -1.5 158,739 + sctN DVUA0119 0.84 +0.7 158,745 - sctN DVUA0119 0.84 +1.7 158,879 + -1.9 158,932 + +0.3 158,937 + -1.3 158,937 + -1.9 158,937 + -1.7 158,937 + +2.8 158,937 + -0.0 158,940 - -0.9 158,945 - -0.7 158,945 - +2.3 158,950 + -1.5 158,950 + +0.1 158,958 - -0.9 158,994 - -1.0 159,039 - DVUA0119.1 0.16 -0.4 159,046 - DVUA0119.1 0.18 -1.4 159,065 - DVUA0119.1 0.21 -1.4 159,162 + DVUA0119.1 0.41 +1.5 159,163 + DVUA0119.1 0.41 -1.2 159,163 + DVUA0119.1 0.41 -1.1 159,170 - DVUA0119.1 0.43 -0.6 159,227 - DVUA0119.1 0.54 -1.6 159,373 - DVUA0119.1 0.84 -0.2 159,414 + -0.4 159,422 - +0.3 159,440 + -0.0 159,521 + +0.3 159,589 - DVUA0120 0.18 +0.7 159,626 + DVUA0120 0.23 +2.2 159,752 + DVUA0120 0.41 -1.0 159,767 + DVUA0120 0.43 -0.4 159,920 - DVUA0120 0.66 +0.1 159,943 + DVUA0120 0.69 +0.4
Or see this region's nucleotide sequence