Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4523 and BT4524 overlap by 14 nucleotides BT4524 and BT4525 are separated by 208 nucleotides BT4525 and BT4526 are separated by 347 nucleotides
BT4523: BT4523 - Type I restriction enzyme EcoR124II specificity protein (NCBI ptt file), at 5,942,349 to 5,943,533
BT4523
BT4524: BT4524 - conserved hypothetical protein (NCBI ptt file), at 5,943,520 to 5,943,810
BT4524
BT4525: BT4525 - hypothetical protein (NCBI ptt file), at 5,944,019 to 5,944,303
BT4525
BT4526: BT4526 - conserved hypothetical protein (NCBI ptt file), at 5,944,651 to 5,945,418
BT4526
Position (kb)
5943
5944 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5942.543 kb on + strand, within BT4523 at 5942.544 kb on - strand, within BT4523 at 5942.544 kb on - strand, within BT4523 at 5942.577 kb on + strand, within BT4523 at 5942.582 kb on + strand, within BT4523 at 5942.632 kb on - strand, within BT4523 at 5942.650 kb on + strand, within BT4523 at 5942.650 kb on + strand, within BT4523 at 5942.651 kb on - strand, within BT4523 at 5942.654 kb on + strand, within BT4523 at 5942.720 kb on - strand, within BT4523 at 5942.732 kb on - strand, within BT4523 at 5942.751 kb on - strand, within BT4523 at 5942.752 kb on + strand, within BT4523 at 5942.752 kb on + strand, within BT4523 at 5942.752 kb on + strand, within BT4523 at 5942.976 kb on + strand, within BT4523 at 5942.984 kb on + strand, within BT4523 at 5942.991 kb on + strand, within BT4523 at 5943.021 kb on + strand, within BT4523 at 5943.030 kb on + strand, within BT4523 at 5943.050 kb on + strand, within BT4523 at 5943.054 kb on - strand, within BT4523 at 5943.081 kb on + strand, within BT4523 at 5943.082 kb on - strand, within BT4523 at 5943.082 kb on - strand, within BT4523 at 5943.143 kb on - strand, within BT4523 at 5943.143 kb on - strand, within BT4523 at 5943.200 kb on + strand, within BT4523 at 5943.201 kb on - strand, within BT4523 at 5943.202 kb on - strand, within BT4523 at 5943.246 kb on + strand, within BT4523 at 5943.279 kb on - strand, within BT4523 at 5943.311 kb on + strand, within BT4523 at 5943.312 kb on - strand, within BT4523 at 5943.355 kb on - strand, within BT4523 at 5943.377 kb on + strand, within BT4523 at 5943.377 kb on + strand, within BT4523 at 5943.378 kb on - strand, within BT4523 at 5943.378 kb on - strand, within BT4523 at 5943.380 kb on - strand, within BT4523 at 5943.425 kb on + strand at 5943.426 kb on - strand at 5943.454 kb on - strand at 5943.533 kb on + strand at 5943.533 kb on + strand at 5943.535 kb on + strand at 5943.536 kb on - strand at 5943.595 kb on + strand, within BT4524 at 5943.595 kb on + strand, within BT4524 at 5943.599 kb on + strand, within BT4524 at 5943.640 kb on + strand, within BT4524 at 5943.670 kb on + strand, within BT4524 at 5943.779 kb on - strand, within BT4524 at 5943.798 kb on + strand at 5943.889 kb on + strand at 5943.978 kb on + strand at 5944.034 kb on + strand at 5944.035 kb on - strand at 5944.037 kb on + strand at 5944.095 kb on + strand, within BT4525 at 5944.096 kb on - strand, within BT4525 at 5944.159 kb on + strand, within BT4525 at 5944.160 kb on - strand, within BT4525 at 5944.266 kb on - strand, within BT4525 at 5944.312 kb on - strand at 5944.318 kb on - strand at 5944.318 kb on - strand at 5944.323 kb on + strand at 5944.324 kb on - strand at 5944.368 kb on - strand at 5944.401 kb on - strand at 5944.401 kb on - strand at 5944.450 kb on + strand at 5944.454 kb on + strand at 5944.454 kb on + strand at 5944.455 kb on - strand at 5944.455 kb on - strand at 5944.458 kb on - strand at 5944.566 kb on + strand at 5944.566 kb on + strand at 5944.571 kb on + strand at 5944.571 kb on + strand at 5944.571 kb on + strand at 5944.572 kb on - strand at 5944.572 kb on - strand at 5944.572 kb on - strand at 5944.572 kb on - strand at 5944.573 kb on + strand at 5944.573 kb on + strand at 5944.573 kb on + strand at 5944.573 kb on + strand at 5944.574 kb on - strand at 5944.603 kb on + strand at 5944.604 kb on - strand at 5944.604 kb on - strand at 5944.605 kb on + strand at 5944.606 kb on - strand at 5944.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 5,942,543 + BT4523 0.16 -0.9 5,942,544 - BT4523 0.16 +1.1 5,942,544 - BT4523 0.16 -0.4 5,942,577 + BT4523 0.19 -0.8 5,942,582 + BT4523 0.20 +1.7 5,942,632 - BT4523 0.24 -1.3 5,942,650 + BT4523 0.25 +0.1 5,942,650 + BT4523 0.25 -1.4 5,942,651 - BT4523 0.25 -0.9 5,942,654 + BT4523 0.26 +1.5 5,942,720 - BT4523 0.31 -0.0 5,942,732 - BT4523 0.32 -1.1 5,942,751 - BT4523 0.34 -1.3 5,942,752 + BT4523 0.34 -1.6 5,942,752 + BT4523 0.34 -1.9 5,942,752 + BT4523 0.34 +0.7 5,942,976 + BT4523 0.53 -0.5 5,942,984 + BT4523 0.54 -0.6 5,942,991 + BT4523 0.54 +0.1 5,943,021 + BT4523 0.57 +0.1 5,943,030 + BT4523 0.57 +1.1 5,943,050 + BT4523 0.59 +0.4 5,943,054 - BT4523 0.59 -1.0 5,943,081 + BT4523 0.62 +0.1 5,943,082 - BT4523 0.62 -0.9 5,943,082 - BT4523 0.62 -0.5 5,943,143 - BT4523 0.67 -0.9 5,943,143 - BT4523 0.67 +1.1 5,943,200 + BT4523 0.72 +0.7 5,943,201 - BT4523 0.72 -1.2 5,943,202 - BT4523 0.72 -1.2 5,943,246 + BT4523 0.76 -2.1 5,943,279 - BT4523 0.78 -0.5 5,943,311 + BT4523 0.81 -2.1 5,943,312 - BT4523 0.81 -0.4 5,943,355 - BT4523 0.85 -0.5 5,943,377 + BT4523 0.87 -0.6 5,943,377 + BT4523 0.87 -1.2 5,943,378 - BT4523 0.87 -1.0 5,943,378 - BT4523 0.87 +0.2 5,943,380 - BT4523 0.87 +0.1 5,943,425 + -1.7 5,943,426 - +0.7 5,943,454 - -1.2 5,943,533 + -1.3 5,943,533 + -2.6 5,943,535 + -0.9 5,943,536 - +1.0 5,943,595 + BT4524 0.26 +0.0 5,943,595 + BT4524 0.26 +0.3 5,943,599 + BT4524 0.27 +1.5 5,943,640 + BT4524 0.41 +0.3 5,943,670 + BT4524 0.52 -0.9 5,943,779 - BT4524 0.89 +0.0 5,943,798 + -1.2 5,943,889 + -2.0 5,943,978 + -1.2 5,944,034 + -1.0 5,944,035 - -0.4 5,944,037 + -0.3 5,944,095 + BT4525 0.27 -1.8 5,944,096 - BT4525 0.27 +0.1 5,944,159 + BT4525 0.49 -1.4 5,944,160 - BT4525 0.49 -0.5 5,944,266 - BT4525 0.87 +0.3 5,944,312 - +1.1 5,944,318 - -0.8 5,944,318 - -0.1 5,944,323 + +1.1 5,944,324 - -0.5 5,944,368 - -2.3 5,944,401 - +0.1 5,944,401 - -1.2 5,944,450 + -1.7 5,944,454 + -1.8 5,944,454 + -1.2 5,944,455 - -0.5 5,944,455 - -0.3 5,944,458 - -1.2 5,944,566 + -0.7 5,944,566 + -0.7 5,944,571 + -1.2 5,944,571 + -0.2 5,944,571 + -0.5 5,944,572 - +0.1 5,944,572 - -0.5 5,944,572 - +0.0 5,944,572 - -0.5 5,944,573 + -1.2 5,944,573 + -0.3 5,944,573 + -0.7 5,944,573 + +0.5 5,944,574 - -1.2 5,944,603 + +2.7 5,944,604 - +1.1 5,944,604 - -0.6 5,944,605 + -0.5 5,944,606 - +3.1 5,944,611 - -1.2
Or see this region's nucleotide sequence