Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3988 and BT3989 are separated by 269 nucleotides BT3989 and BT3990 are separated by 194 nucleotides BT3990 and BT3991 are separated by 97 nucleotides
BT3988: BT3988 - putative peptidoglycan bound protein (NCBI ptt file), at 5,194,400 to 5,195,734
BT3988
BT3989: BT3989 - hypothetical protein (NCBI ptt file), at 5,196,004 to 5,196,438
BT3989
BT3990: BT3990 - putative alpha-1,2-mannosidase (NCBI ptt file), at 5,196,633 to 5,198,900
BT3990
BT3991: BT3991 - putative alpha-1,2-mannosidase (NCBI ptt file), at 5,198,998 to 5,201,274
BT3991
Position (kb)
5196
5197
5198
5199 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5195.644 kb on + strand at 5195.694 kb on - strand at 5195.695 kb on + strand at 5195.695 kb on + strand at 5195.696 kb on - strand at 5195.715 kb on - strand at 5195.720 kb on + strand at 5195.806 kb on + strand at 5195.811 kb on + strand at 5195.811 kb on + strand at 5195.814 kb on + strand at 5195.815 kb on - strand at 5195.925 kb on + strand at 5195.926 kb on - strand at 5195.926 kb on - strand at 5195.926 kb on - strand at 5195.979 kb on + strand at 5195.982 kb on + strand at 5196.027 kb on - strand at 5196.027 kb on - strand at 5196.073 kb on - strand, within BT3989 at 5196.076 kb on + strand, within BT3989 at 5196.077 kb on - strand, within BT3989 at 5196.102 kb on + strand, within BT3989 at 5196.189 kb on + strand, within BT3989 at 5196.190 kb on - strand, within BT3989 at 5196.192 kb on - strand, within BT3989 at 5196.206 kb on - strand, within BT3989 at 5196.230 kb on + strand, within BT3989 at 5196.231 kb on - strand, within BT3989 at 5196.246 kb on + strand, within BT3989 at 5196.258 kb on - strand, within BT3989 at 5196.300 kb on + strand, within BT3989 at 5196.301 kb on - strand, within BT3989 at 5196.342 kb on + strand, within BT3989 at 5196.343 kb on - strand, within BT3989 at 5196.346 kb on + strand, within BT3989 at 5196.417 kb on + strand at 5196.473 kb on + strand at 5196.474 kb on - strand at 5196.474 kb on - strand at 5196.597 kb on - strand at 5196.600 kb on - strand at 5196.602 kb on - strand at 5196.627 kb on + strand at 5196.653 kb on - strand at 5196.698 kb on + strand at 5196.709 kb on - strand at 5196.719 kb on + strand at 5196.720 kb on - strand at 5196.794 kb on + strand at 5196.797 kb on - strand at 5196.815 kb on + strand at 5196.839 kb on + strand at 5196.947 kb on - strand, within BT3990 at 5196.947 kb on - strand, within BT3990 at 5196.985 kb on + strand, within BT3990 at 5197.006 kb on + strand, within BT3990 at 5197.007 kb on - strand, within BT3990 at 5197.021 kb on - strand, within BT3990 at 5197.030 kb on - strand, within BT3990 at 5197.070 kb on - strand, within BT3990 at 5197.111 kb on + strand, within BT3990 at 5197.112 kb on - strand, within BT3990 at 5197.250 kb on + strand, within BT3990 at 5197.250 kb on + strand, within BT3990 at 5197.250 kb on + strand, within BT3990 at 5197.251 kb on - strand, within BT3990 at 5197.251 kb on - strand, within BT3990 at 5197.283 kb on + strand, within BT3990 at 5197.286 kb on - strand, within BT3990 at 5197.495 kb on + strand, within BT3990 at 5197.501 kb on + strand, within BT3990 at 5197.544 kb on - strand, within BT3990 at 5197.549 kb on - strand, within BT3990 at 5197.553 kb on - strand, within BT3990 at 5197.566 kb on - strand, within BT3990 at 5197.567 kb on + strand, within BT3990 at 5197.567 kb on + strand, within BT3990 at 5197.567 kb on + strand, within BT3990 at 5197.642 kb on + strand, within BT3990 at 5197.643 kb on - strand, within BT3990 at 5197.669 kb on - strand, within BT3990 at 5197.669 kb on - strand, within BT3990 at 5197.679 kb on + strand, within BT3990 at 5197.680 kb on - strand, within BT3990 at 5197.774 kb on + strand, within BT3990 at 5197.778 kb on + strand, within BT3990 at 5197.778 kb on + strand, within BT3990 at 5197.778 kb on + strand, within BT3990 at 5197.778 kb on + strand, within BT3990 at 5197.778 kb on + strand, within BT3990 at 5197.806 kb on - strand, within BT3990 at 5197.819 kb on + strand, within BT3990 at 5197.820 kb on - strand, within BT3990 at 5197.883 kb on + strand, within BT3990 at 5197.883 kb on + strand, within BT3990 at 5197.884 kb on - strand, within BT3990 at 5197.885 kb on + strand, within BT3990 at 5197.886 kb on - strand, within BT3990 at 5197.886 kb on - strand, within BT3990 at 5197.896 kb on + strand, within BT3990 at 5197.910 kb on - strand, within BT3990 at 5197.918 kb on + strand, within BT3990 at 5197.994 kb on - strand, within BT3990 at 5198.001 kb on - strand, within BT3990 at 5198.088 kb on + strand, within BT3990 at 5198.157 kb on - strand, within BT3990 at 5198.233 kb on - strand, within BT3990 at 5198.273 kb on - strand, within BT3990 at 5198.364 kb on - strand, within BT3990 at 5198.389 kb on + strand, within BT3990 at 5198.389 kb on + strand, within BT3990 at 5198.389 kb on + strand, within BT3990 at 5198.423 kb on - strand, within BT3990 at 5198.514 kb on + strand, within BT3990 at 5198.521 kb on + strand, within BT3990 at 5198.522 kb on - strand, within BT3990 at 5198.524 kb on + strand, within BT3990 at 5198.527 kb on + strand, within BT3990 at 5198.693 kb on + strand at 5198.693 kb on + strand at 5198.693 kb on + strand at 5198.693 kb on + strand at 5198.694 kb on - strand at 5198.696 kb on - strand at 5198.774 kb on + strand at 5198.827 kb on + strand at 5198.867 kb on + strand at 5198.868 kb on - strand at 5198.872 kb on - strand at 5198.872 kb on - strand at 5198.883 kb on + strand at 5198.884 kb on - strand at 5198.887 kb on - strand at 5198.903 kb on + strand at 5198.907 kb on + strand at 5198.908 kb on - strand at 5198.915 kb on + strand at 5198.918 kb on - strand at 5198.955 kb on + strand at 5199.021 kb on + strand at 5199.022 kb on - strand at 5199.062 kb on - strand at 5199.128 kb on + strand at 5199.222 kb on + strand at 5199.247 kb on + strand, within BT3991 at 5199.254 kb on + strand, within BT3991 at 5199.275 kb on - strand, within BT3991 at 5199.275 kb on - strand, within BT3991 at 5199.276 kb on + strand, within BT3991 at 5199.277 kb on - strand, within BT3991 at 5199.277 kb on - strand, within BT3991 at 5199.278 kb on + strand, within BT3991 at 5199.279 kb on - strand, within BT3991 at 5199.279 kb on - strand, within BT3991 at 5199.279 kb on - strand, within BT3991 at 5199.279 kb on - strand, within BT3991 at 5199.395 kb on + strand, within BT3991 at 5199.401 kb on + strand, within BT3991 at 5199.401 kb on + strand, within BT3991 at 5199.443 kb on + strand, within BT3991 at 5199.620 kb on + strand, within BT3991 at 5199.621 kb on - strand, within BT3991 at 5199.798 kb on + strand, within BT3991 at 5199.799 kb on - strand, within BT3991 at 5199.813 kb on - strand, within BT3991 at 5199.819 kb on - strand, within BT3991 at 5199.867 kb on - strand, within BT3991 at 5199.888 kb on - strand, within BT3991
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 5,195,644 + -1.5 5,195,694 - -2.2 5,195,695 + -1.0 5,195,695 + +0.5 5,195,696 - -2.5 5,195,715 - -0.7 5,195,720 + -1.2 5,195,806 + -0.1 5,195,811 + -1.2 5,195,811 + -0.9 5,195,814 + -1.6 5,195,815 - -1.8 5,195,925 + -0.6 5,195,926 - -1.7 5,195,926 - -0.9 5,195,926 - -1.5 5,195,979 + -0.3 5,195,982 + +0.4 5,196,027 - -0.9 5,196,027 - -1.6 5,196,073 - BT3989 0.16 -0.5 5,196,076 + BT3989 0.17 +0.1 5,196,077 - BT3989 0.17 -1.0 5,196,102 + BT3989 0.23 -1.2 5,196,189 + BT3989 0.43 -0.9 5,196,190 - BT3989 0.43 -0.5 5,196,192 - BT3989 0.43 -1.5 5,196,206 - BT3989 0.46 -1.9 5,196,230 + BT3989 0.52 -1.5 5,196,231 - BT3989 0.52 +0.5 5,196,246 + BT3989 0.56 -1.0 5,196,258 - BT3989 0.58 -0.5 5,196,300 + BT3989 0.68 +0.5 5,196,301 - BT3989 0.68 +0.9 5,196,342 + BT3989 0.78 -1.5 5,196,343 - BT3989 0.78 +0.1 5,196,346 + BT3989 0.79 -1.0 5,196,417 + +2.1 5,196,473 + +1.7 5,196,474 - -0.5 5,196,474 - -0.8 5,196,597 - -1.0 5,196,600 - -3.4 5,196,602 - -1.3 5,196,627 + -0.5 5,196,653 - -0.9 5,196,698 + -1.3 5,196,709 - -1.0 5,196,719 + +0.1 5,196,720 - -0.8 5,196,794 + -0.4 5,196,797 - +1.1 5,196,815 + -0.9 5,196,839 + -0.5 5,196,947 - BT3990 0.14 -0.2 5,196,947 - BT3990 0.14 -1.2 5,196,985 + BT3990 0.16 -1.3 5,197,006 + BT3990 0.16 +0.3 5,197,007 - BT3990 0.16 -0.7 5,197,021 - BT3990 0.17 -0.5 5,197,030 - BT3990 0.18 -1.7 5,197,070 - BT3990 0.19 -1.2 5,197,111 + BT3990 0.21 -1.6 5,197,112 - BT3990 0.21 -0.6 5,197,250 + BT3990 0.27 -0.7 5,197,250 + BT3990 0.27 -0.6 5,197,250 + BT3990 0.27 -0.5 5,197,251 - BT3990 0.27 -2.5 5,197,251 - BT3990 0.27 -0.9 5,197,283 + BT3990 0.29 +0.1 5,197,286 - BT3990 0.29 -0.8 5,197,495 + BT3990 0.38 -0.6 5,197,501 + BT3990 0.38 -1.3 5,197,544 - BT3990 0.40 -0.1 5,197,549 - BT3990 0.40 +0.1 5,197,553 - BT3990 0.41 -1.7 5,197,566 - BT3990 0.41 -1.5 5,197,567 + BT3990 0.41 -0.6 5,197,567 + BT3990 0.41 -0.9 5,197,567 + BT3990 0.41 -1.3 5,197,642 + BT3990 0.44 -0.9 5,197,643 - BT3990 0.45 -1.3 5,197,669 - BT3990 0.46 -0.3 5,197,669 - BT3990 0.46 +1.1 5,197,679 + BT3990 0.46 -0.2 5,197,680 - BT3990 0.46 +1.1 5,197,774 + BT3990 0.50 -0.9 5,197,778 + BT3990 0.50 +0.1 5,197,778 + BT3990 0.50 -1.1 5,197,778 + BT3990 0.50 +0.5 5,197,778 + BT3990 0.50 -0.9 5,197,778 + BT3990 0.50 +0.5 5,197,806 - BT3990 0.52 -1.2 5,197,819 + BT3990 0.52 -1.1 5,197,820 - BT3990 0.52 +0.0 5,197,883 + BT3990 0.55 -1.7 5,197,883 + BT3990 0.55 -2.2 5,197,884 - BT3990 0.55 -0.7 5,197,885 + BT3990 0.55 +0.5 5,197,886 - BT3990 0.55 -0.2 5,197,886 - BT3990 0.55 -1.0 5,197,896 + BT3990 0.56 -0.9 5,197,910 - BT3990 0.56 -0.9 5,197,918 + BT3990 0.57 -2.0 5,197,994 - BT3990 0.60 -1.2 5,198,001 - BT3990 0.60 +1.1 5,198,088 + BT3990 0.64 -1.2 5,198,157 - BT3990 0.67 +0.1 5,198,233 - BT3990 0.71 -0.1 5,198,273 - BT3990 0.72 -0.4 5,198,364 - BT3990 0.76 -1.0 5,198,389 + BT3990 0.77 +1.7 5,198,389 + BT3990 0.77 +1.4 5,198,389 + BT3990 0.77 -1.2 5,198,423 - BT3990 0.79 -1.6 5,198,514 + BT3990 0.83 -0.5 5,198,521 + BT3990 0.83 +0.5 5,198,522 - BT3990 0.83 -1.7 5,198,524 + BT3990 0.83 -1.2 5,198,527 + BT3990 0.84 +0.5 5,198,693 + -1.4 5,198,693 + -0.7 5,198,693 + -0.6 5,198,693 + -1.5 5,198,694 - -0.9 5,198,696 - -2.2 5,198,774 + -0.9 5,198,827 + -1.5 5,198,867 + -1.9 5,198,868 - -0.5 5,198,872 - -0.9 5,198,872 - +1.1 5,198,883 + -0.8 5,198,884 - -1.0 5,198,887 - -0.1 5,198,903 + +0.5 5,198,907 + -0.7 5,198,908 - -0.6 5,198,915 + -1.1 5,198,918 - +1.4 5,198,955 + -2.5 5,199,021 + -1.6 5,199,022 - +1.1 5,199,062 - -0.8 5,199,128 + -1.7 5,199,222 + -0.0 5,199,247 + BT3991 0.11 -2.3 5,199,254 + BT3991 0.11 -0.3 5,199,275 - BT3991 0.12 +1.1 5,199,275 - BT3991 0.12 -1.9 5,199,276 + BT3991 0.12 -2.2 5,199,277 - BT3991 0.12 -0.2 5,199,277 - BT3991 0.12 +1.1 5,199,278 + BT3991 0.12 +0.5 5,199,279 - BT3991 0.12 -0.7 5,199,279 - BT3991 0.12 -0.6 5,199,279 - BT3991 0.12 -0.5 5,199,279 - BT3991 0.12 -0.1 5,199,395 + BT3991 0.17 +0.5 5,199,401 + BT3991 0.18 -0.6 5,199,401 + BT3991 0.18 -0.9 5,199,443 + BT3991 0.20 -2.5 5,199,620 + BT3991 0.27 -0.3 5,199,621 - BT3991 0.27 +1.4 5,199,798 + BT3991 0.35 -1.7 5,199,799 - BT3991 0.35 -0.6 5,199,813 - BT3991 0.36 -0.5 5,199,819 - BT3991 0.36 -1.4 5,199,867 - BT3991 0.38 -1.7 5,199,888 - BT3991 0.39 -0.5
Or see this region's nucleotide sequence