Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3926 and BT3927 are separated by 109 nucleotides BT3927 and BT3928 are separated by 274 nucleotides BT3928 and BT3929 are separated by 29 nucleotides BT3929 and BT3930 are separated by 126 nucleotides
BT3926: BT3926 - putative peptidase (NCBI ptt file), at 5,099,845 to 5,100,717
BT3926
BT3927: BT3927 - conserved hypothetical protein (NCBI ptt file), at 5,100,827 to 5,101,369
BT3927
BT3928: BT3928 - hypothetical protein (NCBI ptt file), at 5,101,644 to 5,102,675
BT3928
BT3929: BT3929 - triosephosphate isomerase (NCBI ptt file), at 5,102,705 to 5,103,463
BT3929
BT3930: BT3930 - hypothetical protein (NCBI ptt file), at 5,103,590 to 5,104,039
BT3930
Position (kb) 5101
5102
5103 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5100.726 kb on + strand at 5100.727 kb on - strand at 5100.734 kb on + strand at 5100.735 kb on - strand at 5100.756 kb on - strand at 5100.795 kb on + strand at 5101.348 kb on + strand at 5101.348 kb on + strand at 5101.375 kb on + strand at 5101.558 kb on + strand at 5101.733 kb on + strand at 5101.762 kb on + strand, within BT3928 at 5101.851 kb on + strand, within BT3928 at 5101.858 kb on + strand, within BT3928 at 5101.884 kb on + strand, within BT3928 at 5101.986 kb on + strand, within BT3928 at 5101.986 kb on + strand, within BT3928 at 5102.033 kb on + strand, within BT3928 at 5102.189 kb on + strand, within BT3928 at 5102.189 kb on + strand, within BT3928 at 5102.189 kb on + strand, within BT3928 at 5102.189 kb on + strand, within BT3928 at 5102.213 kb on + strand, within BT3928 at 5102.257 kb on + strand, within BT3928 at 5102.259 kb on + strand, within BT3928 at 5102.259 kb on + strand, within BT3928 at 5102.259 kb on + strand, within BT3928 at 5102.259 kb on + strand, within BT3928 at 5102.265 kb on + strand, within BT3928 at 5102.292 kb on + strand, within BT3928 at 5102.300 kb on + strand, within BT3928 at 5102.323 kb on + strand, within BT3928 at 5102.325 kb on + strand, within BT3928 at 5102.482 kb on + strand, within BT3928 at 5102.539 kb on + strand, within BT3928 at 5102.543 kb on + strand, within BT3928 at 5102.553 kb on + strand, within BT3928 at 5102.679 kb on + strand at 5103.499 kb on + strand at 5103.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 5,100,726 + -0.2 5,100,727 - -0.7 5,100,734 + -1.3 5,100,735 - -0.8 5,100,756 - -3.0 5,100,795 + +1.7 5,101,348 + +3.6 5,101,348 + +2.2 5,101,375 + +2.1 5,101,558 + +2.1 5,101,733 + +2.3 5,101,762 + BT3928 0.11 +2.5 5,101,851 + BT3928 0.20 +2.2 5,101,858 + BT3928 0.21 -0.1 5,101,884 + BT3928 0.23 +2.5 5,101,986 + BT3928 0.33 +2.6 5,101,986 + BT3928 0.33 +3.1 5,102,033 + BT3928 0.38 +2.2 5,102,189 + BT3928 0.53 +2.5 5,102,189 + BT3928 0.53 +3.0 5,102,189 + BT3928 0.53 -0.1 5,102,189 + BT3928 0.53 +2.6 5,102,213 + BT3928 0.55 -0.4 5,102,257 + BT3928 0.59 +2.6 5,102,259 + BT3928 0.60 +2.3 5,102,259 + BT3928 0.60 +3.1 5,102,259 + BT3928 0.60 -1.2 5,102,259 + BT3928 0.60 +2.6 5,102,265 + BT3928 0.60 +3.6 5,102,292 + BT3928 0.63 +2.4 5,102,300 + BT3928 0.64 +0.4 5,102,323 + BT3928 0.66 +0.7 5,102,325 + BT3928 0.66 +1.9 5,102,482 + BT3928 0.81 +2.1 5,102,539 + BT3928 0.87 +1.0 5,102,543 + BT3928 0.87 +2.4 5,102,553 + BT3928 0.88 +2.9 5,102,679 + +0.9 5,103,499 + +1.7 5,103,502 + +2.6
Or see this region's nucleotide sequence