Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2346 and BT2347 are separated by 378 nucleotides BT2347 and BT2348 are separated by 4 nucleotides BT2348 and BT2349 are separated by 79 nucleotides
BT2346: BT2346 - iron-sulfur cluster-binding protein (NCBI ptt file), at 2,925,082 to 2,925,744
BT2346
BT2347: BT2347 - transposase (NCBI ptt file), at 2,926,123 to 2,926,488
BT2347
BT2348: BT2348 - conserved hypothetical protein, IS related (NCBI ptt file), at 2,926,493 to 2,926,837
BT2348
BT2349: BT2349 - transposase (NCBI ptt file), at 2,926,917 to 2,928,488
BT2349
Position (kb)
2926
2927 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2925.560 kb on - strand, within BT2346 at 2925.584 kb on + strand, within BT2346 at 2925.661 kb on + strand, within BT2346 at 2925.662 kb on - strand, within BT2346 at 2925.662 kb on - strand, within BT2346 at 2925.663 kb on + strand, within BT2346 at 2925.667 kb on - strand, within BT2346 at 2925.672 kb on - strand, within BT2346 at 2925.723 kb on + strand at 2925.742 kb on + strand at 2925.794 kb on + strand at 2925.829 kb on + strand at 2925.877 kb on - strand at 2925.880 kb on + strand at 2925.880 kb on + strand at 2925.881 kb on - strand at 2925.894 kb on - strand at 2926.045 kb on - strand at 2926.216 kb on + strand, within BT2347 at 2926.234 kb on + strand, within BT2347 at 2926.271 kb on - strand, within BT2347 at 2926.271 kb on - strand, within BT2347 at 2926.350 kb on + strand, within BT2347 at 2926.351 kb on - strand, within BT2347 at 2926.356 kb on - strand, within BT2347 at 2926.356 kb on - strand, within BT2347 at 2926.360 kb on - strand, within BT2347 at 2926.360 kb on - strand, within BT2347 at 2926.403 kb on + strand, within BT2347 at 2926.449 kb on - strand, within BT2347 at 2926.449 kb on - strand, within BT2347 at 2926.449 kb on - strand, within BT2347 at 2926.674 kb on + strand, within BT2348 at 2926.831 kb on + strand at 2926.836 kb on - strand at 2926.894 kb on - strand at 2926.936 kb on + strand at 2926.937 kb on - strand at 2926.968 kb on - strand at 2926.968 kb on - strand at 2927.054 kb on - strand at 2927.084 kb on + strand, within BT2349 at 2927.085 kb on - strand, within BT2349 at 2927.201 kb on + strand, within BT2349 at 2927.201 kb on + strand, within BT2349 at 2927.202 kb on - strand, within BT2349 at 2927.202 kb on - strand, within BT2349 at 2927.202 kb on - strand, within BT2349 at 2927.210 kb on + strand, within BT2349 at 2927.211 kb on - strand, within BT2349 at 2927.234 kb on + strand, within BT2349 at 2927.235 kb on - strand, within BT2349 at 2927.272 kb on + strand, within BT2349 at 2927.302 kb on - strand, within BT2349 at 2927.325 kb on - strand, within BT2349 at 2927.325 kb on - strand, within BT2349 at 2927.330 kb on + strand, within BT2349 at 2927.331 kb on - strand, within BT2349 at 2927.357 kb on - strand, within BT2349 at 2927.358 kb on + strand, within BT2349 at 2927.362 kb on + strand, within BT2349 at 2927.362 kb on + strand, within BT2349 at 2927.363 kb on - strand, within BT2349 at 2927.375 kb on - strand, within BT2349 at 2927.375 kb on - strand, within BT2349 at 2927.422 kb on - strand, within BT2349 at 2927.438 kb on - strand, within BT2349 at 2927.438 kb on - strand, within BT2349 at 2927.467 kb on - strand, within BT2349 at 2927.468 kb on + strand, within BT2349 at 2927.523 kb on + strand, within BT2349 at 2927.524 kb on - strand, within BT2349 at 2927.539 kb on - strand, within BT2349 at 2927.548 kb on + strand, within BT2349 at 2927.567 kb on - strand, within BT2349 at 2927.567 kb on - strand, within BT2349 at 2927.626 kb on + strand, within BT2349 at 2927.638 kb on - strand, within BT2349 at 2927.659 kb on + strand, within BT2349 at 2927.659 kb on + strand, within BT2349 at 2927.659 kb on + strand, within BT2349 at 2927.776 kb on + strand, within BT2349 at 2927.776 kb on + strand, within BT2349 at 2927.777 kb on - strand, within BT2349 at 2927.777 kb on - strand, within BT2349 at 2927.777 kb on - strand, within BT2349
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 2,925,560 - BT2346 0.72 -1.1 2,925,584 + BT2346 0.76 -1.0 2,925,661 + BT2346 0.87 +1.2 2,925,662 - BT2346 0.87 -0.8 2,925,662 - BT2346 0.87 -1.8 2,925,663 + BT2346 0.88 +0.1 2,925,667 - BT2346 0.88 -1.6 2,925,672 - BT2346 0.89 -0.7 2,925,723 + -2.3 2,925,742 + -0.5 2,925,794 + -1.4 2,925,829 + -1.5 2,925,877 - +0.6 2,925,880 + -2.0 2,925,880 + -0.4 2,925,881 - +0.2 2,925,894 - -0.7 2,926,045 - -0.5 2,926,216 + BT2347 0.25 -1.4 2,926,234 + BT2347 0.30 -1.3 2,926,271 - BT2347 0.40 -1.5 2,926,271 - BT2347 0.40 +0.2 2,926,350 + BT2347 0.62 -0.4 2,926,351 - BT2347 0.62 -0.1 2,926,356 - BT2347 0.64 -0.8 2,926,356 - BT2347 0.64 -0.9 2,926,360 - BT2347 0.65 -1.4 2,926,360 - BT2347 0.65 -0.7 2,926,403 + BT2347 0.77 +0.2 2,926,449 - BT2347 0.89 -0.9 2,926,449 - BT2347 0.89 +2.8 2,926,449 - BT2347 0.89 -1.8 2,926,674 + BT2348 0.52 -0.3 2,926,831 + -0.7 2,926,836 - -1.4 2,926,894 - -0.2 2,926,936 + -0.8 2,926,937 - +1.2 2,926,968 - -0.6 2,926,968 - -1.3 2,927,054 - -1.3 2,927,084 + BT2349 0.11 +0.8 2,927,085 - BT2349 0.11 -1.1 2,927,201 + BT2349 0.18 -0.7 2,927,201 + BT2349 0.18 -0.3 2,927,202 - BT2349 0.18 -0.8 2,927,202 - BT2349 0.18 -0.8 2,927,202 - BT2349 0.18 -0.9 2,927,210 + BT2349 0.19 +0.2 2,927,211 - BT2349 0.19 -0.8 2,927,234 + BT2349 0.20 +0.3 2,927,235 - BT2349 0.20 +1.2 2,927,272 + BT2349 0.23 +0.5 2,927,302 - BT2349 0.24 -0.9 2,927,325 - BT2349 0.26 -0.8 2,927,325 - BT2349 0.26 -0.8 2,927,330 + BT2349 0.26 -0.9 2,927,331 - BT2349 0.26 -1.1 2,927,357 - BT2349 0.28 -1.7 2,927,358 + BT2349 0.28 -0.8 2,927,362 + BT2349 0.28 -1.1 2,927,362 + BT2349 0.28 -1.0 2,927,363 - BT2349 0.28 -0.8 2,927,375 - BT2349 0.29 -1.3 2,927,375 - BT2349 0.29 -1.1 2,927,422 - BT2349 0.32 -0.7 2,927,438 - BT2349 0.33 +2.8 2,927,438 - BT2349 0.33 -1.8 2,927,467 - BT2349 0.35 +0.0 2,927,468 + BT2349 0.35 -0.9 2,927,523 + BT2349 0.39 +0.6 2,927,524 - BT2349 0.39 -1.0 2,927,539 - BT2349 0.40 -2.8 2,927,548 + BT2349 0.40 -0.8 2,927,567 - BT2349 0.41 -0.5 2,927,567 - BT2349 0.41 -2.3 2,927,626 + BT2349 0.45 -0.4 2,927,638 - BT2349 0.46 +0.8 2,927,659 + BT2349 0.47 +0.2 2,927,659 + BT2349 0.47 -1.2 2,927,659 + BT2349 0.47 +2.2 2,927,776 + BT2349 0.55 -1.1 2,927,776 + BT2349 0.55 -0.4 2,927,777 - BT2349 0.55 -1.2 2,927,777 - BT2349 0.55 -1.3 2,927,777 - BT2349 0.55 -2.2
Or see this region's nucleotide sequence