Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2168

Experiment: BHIS with Trimethoprim 7.5 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2167 and BT2168 are separated by 257 nucleotidesBT2168 and BT2169 are separated by 19 nucleotidesBT2169 and BT2170 are separated by 134 nucleotides BT2167: BT2167 - elongation factor G (NCBI ptt file), at 2,725,620 to 2,727,776 BT2167 BT2168: BT2168 - coproporphyrinogen III oxidase (NCBI ptt file), at 2,728,034 to 2,729,164 BT2168 BT2169: BT2169 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,729,184 to 2,729,732 BT2169 BT2170: BT2170 - hypothetical protein (NCBI ptt file), at 2,729,867 to 2,730,301 BT2170 Position (kb) 2728 2729 2730Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2727.138 kb on + strand, within BT2167at 2727.246 kb on + strand, within BT2167at 2727.317 kb on - strand, within BT2167at 2727.341 kb on + strandat 2727.366 kb on - strand, within BT2167at 2727.390 kb on - strand, within BT2167at 2727.398 kb on + strand, within BT2167at 2727.416 kb on - strand, within BT2167at 2727.491 kb on + strand, within BT2167at 2727.540 kb on + strand, within BT2167at 2727.545 kb on + strand, within BT2167at 2727.546 kb on - strand, within BT2167at 2727.605 kb on - strandat 2727.776 kb on + strandat 2727.777 kb on - strandat 2727.779 kb on + strandat 2727.780 kb on - strandat 2727.782 kb on - strandat 2727.824 kb on + strandat 2727.884 kb on + strandat 2727.901 kb on + strandat 2727.902 kb on - strandat 2727.918 kb on + strandat 2727.918 kb on + strandat 2727.943 kb on - strandat 2727.948 kb on + strandat 2727.948 kb on + strandat 2727.948 kb on + strandat 2728.002 kb on - strandat 2728.003 kb on + strandat 2728.033 kb on + strandat 2728.046 kb on + strandat 2728.054 kb on + strandat 2728.066 kb on + strandat 2728.083 kb on - strandat 2728.128 kb on - strandat 2728.199 kb on + strand, within BT2168at 2728.350 kb on + strand, within BT2168at 2728.433 kb on - strand, within BT2168at 2728.622 kb on - strand, within BT2168at 2728.622 kb on - strand, within BT2168at 2728.625 kb on + strand, within BT2168at 2728.687 kb on + strand, within BT2168at 2728.722 kb on - strand, within BT2168at 2728.763 kb on + strand, within BT2168at 2728.777 kb on + strand, within BT2168at 2728.797 kb on + strand, within BT2168at 2728.798 kb on - strand, within BT2168at 2728.802 kb on + strand, within BT2168at 2728.803 kb on - strand, within BT2168at 2728.875 kb on + strand, within BT2168at 2728.877 kb on + strand, within BT2168at 2728.890 kb on + strand, within BT2168at 2728.890 kb on + strand, within BT2168at 2728.968 kb on + strand, within BT2168at 2728.969 kb on - strand, within BT2168at 2728.992 kb on + strand, within BT2168at 2729.057 kb on + strandat 2729.097 kb on + strandat 2729.137 kb on + strandat 2729.163 kb on - strandat 2729.259 kb on + strand, within BT2169at 2729.259 kb on + strand, within BT2169at 2729.260 kb on - strand, within BT2169at 2729.260 kb on - strand, within BT2169at 2729.260 kb on - strand, within BT2169at 2729.261 kb on + strand, within BT2169at 2729.271 kb on + strand, within BT2169at 2729.296 kb on - strand, within BT2169at 2729.301 kb on + strand, within BT2169at 2729.461 kb on - strand, within BT2169at 2729.662 kb on + strand, within BT2169at 2729.683 kb on + strandat 2729.683 kb on + strandat 2729.683 kb on + strandat 2729.770 kb on + strandat 2729.825 kb on + strandat 2729.879 kb on - strandat 2729.910 kb on + strandat 2729.958 kb on - strand, within BT2170at 2729.963 kb on - strand, within BT2170at 2729.970 kb on - strand, within BT2170at 2729.970 kb on - strand, within BT2170at 2729.970 kb on - strand, within BT2170at 2730.125 kb on - strand, within BT2170

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM
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2,727,138 + BT2167 0.70 -0.6
2,727,246 + BT2167 0.75 -2.1
2,727,317 - BT2167 0.79 +1.6
2,727,341 + -0.4
2,727,366 - BT2167 0.81 -0.3
2,727,390 - BT2167 0.82 -0.1
2,727,398 + BT2167 0.82 -0.9
2,727,416 - BT2167 0.83 -0.3
2,727,491 + BT2167 0.87 +0.4
2,727,540 + BT2167 0.89 +0.7
2,727,545 + BT2167 0.89 -2.2
2,727,546 - BT2167 0.89 -1.8
2,727,605 - -0.7
2,727,776 + -1.0
2,727,777 - +0.2
2,727,779 + -1.5
2,727,780 - +0.2
2,727,782 - -0.6
2,727,824 + +1.2
2,727,884 + +1.2
2,727,901 + +2.0
2,727,902 - -1.1
2,727,918 + +0.7
2,727,918 + +0.9
2,727,943 - -1.0
2,727,948 + -2.3
2,727,948 + -1.7
2,727,948 + -0.8
2,728,002 - -0.7
2,728,003 + -2.8
2,728,033 + +0.9
2,728,046 + +0.7
2,728,054 + +1.2
2,728,066 + -2.0
2,728,083 - -2.5
2,728,128 - -0.9
2,728,199 + BT2168 0.15 -0.6
2,728,350 + BT2168 0.28 -0.0
2,728,433 - BT2168 0.35 -0.3
2,728,622 - BT2168 0.52 -1.7
2,728,622 - BT2168 0.52 +0.8
2,728,625 + BT2168 0.52 -0.1
2,728,687 + BT2168 0.58 +1.2
2,728,722 - BT2168 0.61 +0.2
2,728,763 + BT2168 0.64 +1.2
2,728,777 + BT2168 0.66 -1.3
2,728,797 + BT2168 0.67 +0.4
2,728,798 - BT2168 0.68 +0.3
2,728,802 + BT2168 0.68 +2.8
2,728,803 - BT2168 0.68 -0.2
2,728,875 + BT2168 0.74 -1.8
2,728,877 + BT2168 0.75 -0.2
2,728,890 + BT2168 0.76 -0.2
2,728,890 + BT2168 0.76 +0.2
2,728,968 + BT2168 0.83 -2.2
2,728,969 - BT2168 0.83 -0.2
2,728,992 + BT2168 0.85 +0.5
2,729,057 + -0.9
2,729,097 + -1.9
2,729,137 + -0.9
2,729,163 - +0.4
2,729,259 + BT2169 0.14 -0.8
2,729,259 + BT2169 0.14 +1.8
2,729,260 - BT2169 0.14 -0.1
2,729,260 - BT2169 0.14 -0.9
2,729,260 - BT2169 0.14 -1.2
2,729,261 + BT2169 0.14 -0.8
2,729,271 + BT2169 0.16 -1.6
2,729,296 - BT2169 0.20 -0.3
2,729,301 + BT2169 0.21 -0.1
2,729,461 - BT2169 0.50 -0.7
2,729,662 + BT2169 0.87 +0.7
2,729,683 + +0.5
2,729,683 + -0.9
2,729,683 + -0.0
2,729,770 + -0.5
2,729,825 + -0.1
2,729,879 - -1.0
2,729,910 + +2.2
2,729,958 - BT2170 0.21 -1.4
2,729,963 - BT2170 0.22 -1.1
2,729,970 - BT2170 0.24 -0.8
2,729,970 - BT2170 0.24 -1.2
2,729,970 - BT2170 0.24 -1.1
2,730,125 - BT2170 0.59 -0.3

Or see this region's nucleotide sequence