Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0543 and BT0544 are separated by 55 nucleotides BT0544 and BT0545 are separated by 82 nucleotides BT0545 and BT0546 are separated by 151 nucleotides
BT0543: BT0543 - glutamine synthetase (NCBI ptt file), at 667,987 to 670,176
BT0543
BT0544: BT0544 - ammonium transporter (NCBI ptt file), at 670,232 to 671,689
BT0544
BT0545: BT0545 - nitrogen regulatory protein P-II (NCBI ptt file), at 671,772 to 672,128
BT0545
BT0546: BT0546 - conserved hypothetical protein (NCBI ptt file), at 672,280 to 673,005
BT0546
Position (kb) 670
671
672 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 669.417 kb on - strand, within BT0543 at 669.482 kb on - strand, within BT0543 at 669.661 kb on - strand, within BT0543 at 669.662 kb on + strand, within BT0543 at 669.663 kb on - strand, within BT0543 at 669.806 kb on - strand, within BT0543 at 669.806 kb on - strand, within BT0543 at 670.009 kb on - strand at 670.029 kb on - strand at 670.077 kb on - strand at 670.080 kb on + strand at 670.080 kb on + strand at 670.150 kb on + strand at 670.176 kb on + strand at 670.176 kb on + strand at 670.199 kb on + strand at 670.239 kb on + strand at 670.239 kb on + strand at 670.239 kb on + strand at 670.521 kb on - strand, within BT0544 at 670.531 kb on + strand, within BT0544 at 670.532 kb on - strand, within BT0544 at 670.557 kb on - strand, within BT0544 at 670.599 kb on - strand, within BT0544 at 670.835 kb on + strand, within BT0544 at 670.924 kb on + strand, within BT0544 at 670.925 kb on - strand, within BT0544 at 671.099 kb on - strand, within BT0544 at 671.104 kb on + strand, within BT0544 at 671.105 kb on - strand, within BT0544 at 671.107 kb on + strand, within BT0544 at 671.110 kb on - strand, within BT0544 at 671.118 kb on + strand, within BT0544 at 671.548 kb on + strand at 671.636 kb on - strand at 671.696 kb on + strand at 671.697 kb on - strand at 671.938 kb on - strand, within BT0545 at 671.945 kb on + strand, within BT0545 at 672.000 kb on - strand, within BT0545 at 672.168 kb on + strand at 672.201 kb on - strand at 672.201 kb on - strand at 672.201 kb on - strand at 672.227 kb on + strand at 672.248 kb on + strand at 672.306 kb on - strand at 672.318 kb on + strand at 672.329 kb on - strand at 672.472 kb on + strand, within BT0546 at 672.473 kb on - strand, within BT0546 at 672.531 kb on - strand, within BT0546 at 672.631 kb on + strand, within BT0546 at 672.656 kb on + strand, within BT0546
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 669,417 - BT0543 0.65 +1.6 669,482 - BT0543 0.68 -1.3 669,661 - BT0543 0.76 +0.0 669,662 + BT0543 0.76 +0.2 669,663 - BT0543 0.77 +1.0 669,806 - BT0543 0.83 +0.0 669,806 - BT0543 0.83 -0.6 670,009 - +0.0 670,029 - -1.0 670,077 - +1.6 670,080 + +2.0 670,080 + -2.0 670,150 + -1.7 670,176 + +0.0 670,176 + +0.5 670,199 + -1.2 670,239 + -0.8 670,239 + -1.3 670,239 + -0.4 670,521 - BT0544 0.20 -1.4 670,531 + BT0544 0.21 +0.0 670,532 - BT0544 0.21 +1.8 670,557 - BT0544 0.22 +1.9 670,599 - BT0544 0.25 +1.8 670,835 + BT0544 0.41 -0.8 670,924 + BT0544 0.47 -0.4 670,925 - BT0544 0.48 -0.1 671,099 - BT0544 0.59 -1.0 671,104 + BT0544 0.60 -1.0 671,105 - BT0544 0.60 -1.1 671,107 + BT0544 0.60 +0.1 671,110 - BT0544 0.60 -0.6 671,118 + BT0544 0.61 +0.0 671,548 + +0.4 671,636 - -1.3 671,696 + -1.7 671,697 - -0.1 671,938 - BT0545 0.46 +0.4 671,945 + BT0545 0.48 -0.5 672,000 - BT0545 0.64 +0.0 672,168 + -2.4 672,201 - -0.3 672,201 - -0.3 672,201 - +0.9 672,227 + +0.6 672,248 + -0.9 672,306 - -0.6 672,318 + -0.2 672,329 - -0.4 672,472 + BT0546 0.26 +0.0 672,473 - BT0546 0.27 +0.6 672,531 - BT0546 0.35 +0.4 672,631 + BT0546 0.48 -1.0 672,656 + BT0546 0.52 -2.8
Or see this region's nucleotide sequence