Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0173 and BT0174 are separated by 19 nucleotides BT0174 and BT0175 are separated by 238 nucleotides
BT0173: BT0173 - conserved hypothetical protein (NCBI ptt file), at 181,926 to 182,936
BT0173
BT0174: BT0174 - conserved hypothetical protein (NCBI ptt file), at 182,956 to 183,657
BT0174
BT0175: BT0175 - conserved hypothetical protein (NCBI ptt file), at 183,896 to 184,300
BT0175
Position (kb) 181
182
183 Strain fitness (log2 ratio) -2
-1
0
1
2 at 180.935 kb on + strand at 180.990 kb on + strand at 180.998 kb on + strand at 181.018 kb on - strand at 181.035 kb on - strand at 181.053 kb on + strand at 181.055 kb on + strand at 181.057 kb on + strand at 181.096 kb on - strand at 181.104 kb on + strand at 181.122 kb on + strand at 181.141 kb on - strand at 181.147 kb on - strand at 181.151 kb on + strand at 181.152 kb on - strand at 181.153 kb on + strand at 181.153 kb on + strand at 181.154 kb on - strand at 181.154 kb on - strand at 181.260 kb on - strand at 181.346 kb on + strand at 181.346 kb on + strand at 181.484 kb on - strand at 181.501 kb on + strand at 181.519 kb on + strand at 181.536 kb on - strand at 181.536 kb on - strand at 181.565 kb on + strand at 181.566 kb on - strand at 181.621 kb on - strand at 181.655 kb on + strand at 181.713 kb on + strand at 181.768 kb on - strand at 181.787 kb on + strand at 181.790 kb on - strand at 181.790 kb on - strand at 181.790 kb on - strand at 181.795 kb on + strand at 181.818 kb on + strand at 181.827 kb on + strand at 181.828 kb on - strand at 181.895 kb on - strand at 181.905 kb on - strand at 182.096 kb on - strand, within BT0173 at 182.096 kb on - strand, within BT0173 at 182.188 kb on - strand, within BT0173 at 182.203 kb on + strand, within BT0173 at 182.208 kb on - strand, within BT0173 at 182.414 kb on - strand, within BT0173 at 182.741 kb on + strand, within BT0173 at 182.936 kb on + strand at 182.953 kb on - strand at 182.953 kb on - strand at 183.014 kb on + strand at 183.195 kb on - strand, within BT0174 at 183.226 kb on - strand, within BT0174 at 183.402 kb on + strand, within BT0174 at 183.471 kb on + strand, within BT0174 at 183.651 kb on - strand at 183.693 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 180,935 + -0.8 180,990 + +0.7 180,998 + -0.9 181,018 - -1.4 181,035 - +1.1 181,053 + -0.1 181,055 + -0.2 181,057 + -0.5 181,096 - +0.6 181,104 + -0.9 181,122 + -0.4 181,141 - -0.9 181,147 - +0.1 181,151 + -2.3 181,152 - -0.5 181,153 + -0.5 181,153 + +0.1 181,154 - -2.1 181,154 - +0.6 181,260 - -0.4 181,346 + -2.3 181,346 + +0.5 181,484 - -0.7 181,501 + +0.3 181,519 + +0.5 181,536 - -0.6 181,536 - -2.5 181,565 + -1.1 181,566 - +2.1 181,621 - -1.2 181,655 + -1.1 181,713 + -0.5 181,768 - -2.4 181,787 + -0.2 181,790 - +0.1 181,790 - -1.9 181,790 - -2.1 181,795 + -0.5 181,818 + -1.3 181,827 + -1.8 181,828 - -2.3 181,895 - +1.3 181,905 - -1.5 182,096 - BT0173 0.17 -1.3 182,096 - BT0173 0.17 +1.7 182,188 - BT0173 0.26 +2.6 182,203 + BT0173 0.27 +1.1 182,208 - BT0173 0.28 +1.1 182,414 - BT0173 0.48 +0.6 182,741 + BT0173 0.81 +2.1 182,936 + +2.6 182,953 - +1.9 182,953 - -0.7 183,014 + +1.8 183,195 - BT0174 0.34 +0.1 183,226 - BT0174 0.38 -0.5 183,402 + BT0174 0.64 +1.6 183,471 + BT0174 0.73 +1.1 183,651 - -0.9 183,693 - +0.6
Or see this region's nucleotide sequence