Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0055 and BT0056 are separated by 20 nucleotides BT0056 and BT0057 overlap by 11 nucleotides BT0057 and BT0058 are separated by 71 nucleotides
BT0055: BT0055 - putative glycosyltransferase (NCBI ptt file), at 51,140 to 52,255
BT0055
BT0056: BT0056 - putative glycosyltransferase (NCBI ptt file), at 52,276 to 53,298
BT0056
BT0057: BT0057 - hypothetical protein (NCBI ptt file), at 53,288 to 53,575
BT0057
BT0058: BT0058 - hypothetical protein (NCBI ptt file), at 53,647 to 54,777
BT0058
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 51.355 kb on - strand, within BT0055 at 51.490 kb on - strand, within BT0055 at 51.490 kb on - strand, within BT0055 at 51.532 kb on + strand, within BT0055 at 51.580 kb on - strand, within BT0055 at 51.582 kb on - strand, within BT0055 at 51.586 kb on - strand, within BT0055 at 51.714 kb on + strand, within BT0055 at 51.715 kb on - strand, within BT0055 at 51.715 kb on - strand, within BT0055 at 51.792 kb on - strand, within BT0055 at 51.792 kb on - strand at 51.825 kb on - strand, within BT0055 at 52.027 kb on - strand, within BT0055 at 52.027 kb on - strand, within BT0055 at 52.205 kb on - strand at 52.205 kb on - strand at 52.282 kb on - strand at 52.284 kb on - strand at 52.400 kb on + strand, within BT0056 at 52.416 kb on + strand, within BT0056 at 52.444 kb on + strand, within BT0056 at 52.444 kb on + strand, within BT0056 at 52.527 kb on - strand, within BT0056 at 52.527 kb on - strand, within BT0056 at 52.587 kb on - strand, within BT0056 at 52.721 kb on + strand, within BT0056 at 52.721 kb on + strand, within BT0056 at 52.721 kb on + strand, within BT0056 at 52.764 kb on - strand, within BT0056 at 52.794 kb on - strand, within BT0056 at 52.931 kb on - strand, within BT0056 at 52.931 kb on - strand, within BT0056 at 53.086 kb on + strand, within BT0056 at 53.114 kb on + strand, within BT0056 at 53.121 kb on + strand, within BT0056 at 53.121 kb on + strand, within BT0056 at 53.123 kb on + strand, within BT0056 at 53.124 kb on - strand, within BT0056 at 53.126 kb on - strand, within BT0056 at 53.150 kb on - strand, within BT0056 at 53.221 kb on - strand at 53.233 kb on - strand at 53.478 kb on - strand, within BT0057 at 53.478 kb on - strand, within BT0057 at 53.668 kb on - strand at 53.740 kb on - strand at 53.919 kb on - strand, within BT0058 at 53.963 kb on + strand, within BT0058 at 54.131 kb on - strand, within BT0058
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 51,355 - BT0055 0.19 +1.1 51,490 - BT0055 0.31 -1.4 51,490 - BT0055 0.31 +1.1 51,532 + BT0055 0.35 -0.8 51,580 - BT0055 0.39 +1.5 51,582 - BT0055 0.40 -0.2 51,586 - BT0055 0.40 -0.4 51,714 + BT0055 0.51 -0.5 51,715 - BT0055 0.52 -0.5 51,715 - BT0055 0.52 -0.5 51,792 - BT0055 0.58 -0.5 51,792 - -0.9 51,825 - BT0055 0.61 -0.1 52,027 - BT0055 0.79 -1.7 52,027 - BT0055 0.79 -1.1 52,205 - +0.1 52,205 - -1.0 52,282 - +0.6 52,284 - +1.6 52,400 + BT0056 0.12 +2.6 52,416 + BT0056 0.14 +0.1 52,444 + BT0056 0.16 -0.3 52,444 + BT0056 0.16 +0.1 52,527 - BT0056 0.25 -0.4 52,527 - BT0056 0.25 +0.2 52,587 - BT0056 0.30 +0.6 52,721 + BT0056 0.43 +1.1 52,721 + BT0056 0.43 +0.3 52,721 + BT0056 0.43 -0.6 52,764 - BT0056 0.48 -0.1 52,794 - BT0056 0.51 -1.7 52,931 - BT0056 0.64 +2.4 52,931 - BT0056 0.64 -0.5 53,086 + BT0056 0.79 +0.3 53,114 + BT0056 0.82 -0.7 53,121 + BT0056 0.83 +1.1 53,121 + BT0056 0.83 +0.5 53,123 + BT0056 0.83 +3.1 53,124 - BT0056 0.83 -1.3 53,126 - BT0056 0.83 -0.5 53,150 - BT0056 0.85 -2.7 53,221 - -1.9 53,233 - +0.1 53,478 - BT0057 0.66 -1.4 53,478 - BT0057 0.66 +0.4 53,668 - -0.7 53,740 - -2.3 53,919 - BT0058 0.24 +0.3 53,963 + BT0058 0.28 -0.5 54,131 - BT0058 0.43 +1.1
Or see this region's nucleotide sequence