Experiment: BHIS with Trimethoprim 7.5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0031 and BT0032 are separated by 13 nucleotides BT0032 and BT0033 are separated by 273 nucleotides BT0033 and BT0034 are separated by 310 nucleotides
BT0031: BT0031 - conserved hypothetical protein (NCBI ptt file), at 28,131 to 29,195
BT0031
BT0032: BT0032 - hypothetical protein (NCBI ptt file), at 29,209 to 29,406
BT0032
BT0033: BT0033 - hypothetical protein (NCBI ptt file), at 29,680 to 29,970
BT0033
BT0034: BT0034 - hypothetical protein (NCBI ptt file), at 30,281 to 31,492
BT0034
Position (kb)
29
30 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 28.713 kb on - strand, within BT0031 at 28.762 kb on + strand, within BT0031 at 28.818 kb on - strand, within BT0031 at 28.833 kb on + strand, within BT0031 at 28.835 kb on + strand at 28.836 kb on - strand, within BT0031 at 28.836 kb on - strand, within BT0031 at 28.874 kb on - strand, within BT0031 at 28.877 kb on + strand, within BT0031 at 28.877 kb on + strand, within BT0031 at 28.878 kb on - strand, within BT0031 at 28.926 kb on - strand, within BT0031 at 29.025 kb on - strand, within BT0031 at 29.027 kb on + strand, within BT0031 at 29.147 kb on - strand at 29.147 kb on - strand at 29.147 kb on - strand at 29.208 kb on - strand at 29.303 kb on + strand, within BT0032 at 29.304 kb on - strand, within BT0032 at 29.405 kb on - strand at 29.409 kb on + strand at 29.409 kb on + strand at 29.466 kb on + strand at 29.467 kb on - strand at 29.539 kb on + strand at 29.540 kb on - strand at 29.545 kb on + strand at 29.550 kb on + strand at 29.608 kb on - strand at 29.608 kb on - strand at 29.609 kb on + strand at 29.635 kb on - strand at 29.663 kb on + strand at 29.679 kb on + strand at 29.727 kb on + strand, within BT0033 at 29.728 kb on - strand, within BT0033 at 29.819 kb on + strand, within BT0033 at 29.886 kb on + strand, within BT0033 at 29.892 kb on - strand, within BT0033 at 29.910 kb on - strand, within BT0033 at 29.955 kb on + strand at 29.955 kb on + strand at 29.973 kb on + strand at 30.009 kb on + strand at 30.039 kb on + strand at 30.069 kb on - strand at 30.139 kb on + strand at 30.145 kb on + strand at 30.234 kb on + strand at 30.335 kb on - strand at 30.363 kb on - strand at 30.699 kb on + strand, within BT0034 at 30.700 kb on - strand, within BT0034 at 30.700 kb on - strand, within BT0034 at 30.749 kb on + strand, within BT0034 at 30.793 kb on + strand, within BT0034 at 30.804 kb on + strand at 30.872 kb on - strand, within BT0034 at 30.876 kb on + strand, within BT0034
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Trimethoprim 7.5 uM remove 28,713 - BT0031 0.55 -0.7 28,762 + BT0031 0.59 -1.7 28,818 - BT0031 0.65 -0.1 28,833 + BT0031 0.66 +0.3 28,835 + +2.6 28,836 - BT0031 0.66 -1.7 28,836 - BT0031 0.66 -0.2 28,874 - BT0031 0.70 -1.3 28,877 + BT0031 0.70 +1.4 28,877 + BT0031 0.70 -0.2 28,878 - BT0031 0.70 -1.3 28,926 - BT0031 0.75 -0.9 29,025 - BT0031 0.84 -0.5 29,027 + BT0031 0.84 -0.3 29,147 - -0.9 29,147 - -1.2 29,147 - -0.5 29,208 - -0.3 29,303 + BT0032 0.47 -0.7 29,304 - BT0032 0.48 -1.1 29,405 - -2.3 29,409 + +0.2 29,409 + -1.1 29,466 + +0.7 29,467 - -1.0 29,539 + -1.6 29,540 - -0.1 29,545 + -2.9 29,550 + +2.1 29,608 - -1.5 29,608 - -1.3 29,609 + -1.2 29,635 - -2.2 29,663 + -0.5 29,679 + -0.7 29,727 + BT0033 0.16 +0.3 29,728 - BT0033 0.16 -0.5 29,819 + BT0033 0.48 -0.9 29,886 + BT0033 0.71 +0.6 29,892 - BT0033 0.73 +0.1 29,910 - BT0033 0.79 +0.1 29,955 + -0.7 29,955 + -1.6 29,973 + -1.3 30,009 + +0.1 30,039 + -0.7 30,069 - +0.8 30,139 + -0.8 30,145 + -0.9 30,234 + -1.7 30,335 - -0.7 30,363 - +0.1 30,699 + BT0034 0.34 -1.5 30,700 - BT0034 0.35 +0.1 30,700 - BT0034 0.35 +0.1 30,749 + BT0034 0.39 -0.7 30,793 + BT0034 0.42 +1.1 30,804 + +0.3 30,872 - BT0034 0.49 -0.0 30,876 + BT0034 0.49 -0.3
Or see this region's nucleotide sequence