Experiment: MoYLS4 with Manganese Chloride 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2013 and fprA-1 are separated by 74 nucleotides fprA-1 and DORF15009 overlap by 4 nucleotides DORF15009 and DVU2016 overlap by 115 nucleotides
DVU2013: DVU2013 - hybrid cluster protein (TIGR), at 2,090,668 to 2,092,329
DVU2013
DVU2014: fprA-1 - metallo-beta-lactamase family protein (TIGR), at 2,092,404 to 2,093,579
fprA-1
DORF15009: DORF15009 - SEED: Response regulator containing a CheY-like receiver domain and a GGDEF domain, at 2,093,576 to 2,093,878
DORF15009
DVU2016: DVU2016 - GGDEF domain protein (TIGR), at 2,093,764 to 2,094,645
DVU2016
Position (kb)
2092
2093
2094 Strain fitness (log2 ratio)
-1
0
1
2 at 2091.411 kb on + strand, within DVU2013 at 2091.536 kb on + strand, within DVU2013 at 2091.544 kb on - strand, within DVU2013 at 2091.602 kb on - strand, within DVU2013 at 2091.672 kb on - strand, within DVU2013 at 2091.714 kb on - strand, within DVU2013 at 2091.840 kb on + strand, within DVU2013 at 2091.880 kb on - strand, within DVU2013 at 2091.895 kb on + strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.961 kb on - strand, within DVU2013 at 2092.120 kb on - strand, within DVU2013 at 2092.162 kb on - strand, within DVU2013 at 2092.274 kb on + strand at 2092.379 kb on + strand at 2092.458 kb on + strand at 2092.491 kb on + strand at 2092.500 kb on - strand at 2092.500 kb on - strand at 2092.500 kb on - strand at 2092.502 kb on + strand at 2092.502 kb on + strand at 2092.517 kb on - strand at 2092.648 kb on + strand, within fprA-1 at 2092.665 kb on + strand, within fprA-1 at 2092.812 kb on - strand, within fprA-1 at 2092.862 kb on - strand, within fprA-1 at 2092.911 kb on + strand, within fprA-1 at 2093.082 kb on + strand, within fprA-1 at 2093.167 kb on - strand, within fprA-1 at 2093.256 kb on + strand, within fprA-1 at 2093.272 kb on - strand, within fprA-1 at 2093.286 kb on + strand, within fprA-1 at 2093.313 kb on - strand, within fprA-1 at 2093.399 kb on - strand, within fprA-1 at 2093.424 kb on - strand, within fprA-1 at 2093.504 kb on + strand at 2094.049 kb on + strand, within DVU2016 at 2094.391 kb on + strand, within DVU2016 at 2094.391 kb on + strand, within DVU2016
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Manganese Chloride 100uM remove 2,091,411 + DVU2013 0.45 +1.5 2,091,536 + DVU2013 0.52 +0.6 2,091,544 - DVU2013 0.53 -0.4 2,091,602 - DVU2013 0.56 +1.9 2,091,672 - DVU2013 0.60 -0.8 2,091,714 - DVU2013 0.63 -0.3 2,091,840 + DVU2013 0.71 +0.1 2,091,880 - DVU2013 0.73 -0.5 2,091,895 + DVU2013 0.74 -0.1 2,091,940 - DVU2013 0.77 -0.2 2,091,940 - DVU2013 0.77 -0.5 2,091,940 - DVU2013 0.77 -0.5 2,091,940 - DVU2013 0.77 -1.7 2,091,961 - DVU2013 0.78 -0.2 2,092,120 - DVU2013 0.87 -0.5 2,092,162 - DVU2013 0.90 -0.2 2,092,274 + -0.1 2,092,379 + -0.0 2,092,458 + -0.7 2,092,491 + -1.6 2,092,500 - -0.4 2,092,500 - -0.0 2,092,500 - -0.1 2,092,502 + -0.8 2,092,502 + -0.9 2,092,517 - -1.0 2,092,648 + fprA-1 DVU2014 0.21 -0.2 2,092,665 + fprA-1 DVU2014 0.22 -0.9 2,092,812 - fprA-1 DVU2014 0.35 -1.7 2,092,862 - fprA-1 DVU2014 0.39 +0.0 2,092,911 + fprA-1 DVU2014 0.43 -0.1 2,093,082 + fprA-1 DVU2014 0.58 -1.1 2,093,167 - fprA-1 DVU2014 0.65 -1.3 2,093,256 + fprA-1 DVU2014 0.72 -0.9 2,093,272 - fprA-1 DVU2014 0.74 +1.3 2,093,286 + fprA-1 DVU2014 0.75 +0.6 2,093,313 - fprA-1 DVU2014 0.77 -1.0 2,093,399 - fprA-1 DVU2014 0.85 -1.6 2,093,424 - fprA-1 DVU2014 0.87 -1.3 2,093,504 + -0.3 2,094,049 + DVU2016 0.32 -0.1 2,094,391 + DVU2016 0.71 +0.0 2,094,391 + DVU2016 0.71 -0.6
Or see this region's nucleotide sequence