Experiment: MoYLS4 with Manganese Chloride 10uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypA and hypB are separated by 10 nucleotides hypB and E.coliMinDlike) overlap by 4 nucleotides E.coliMinDlike) and DVU2331 are separated by 302 nucleotides
DVU2328: hypA - hydrogenase nickel insertion protein HypA, putative (TIGR), at 2,424,814 to 2,425,167
hypA
DVU2329: hypB - hydrogenase accessory protein HypB (TIGR), at 2,425,178 to 2,425,852
hypB
DVU2330: E.coliMinDlike) - MRP family protein (TIGR), at 2,425,849 to 2,426,730
E.coliMinDlike)
DVU2331: DVU2331 - Smr family protein (TIGR), at 2,427,033 to 2,428,262
DVU2331
Position (kb)
2425
2426
2427 Strain fitness (log2 ratio)
-1
0
1 at 2424.953 kb on + strand, within hypA at 2425.004 kb on - strand, within hypA at 2425.064 kb on + strand, within hypA at 2425.123 kb on + strand, within hypA at 2425.152 kb on + strand at 2425.813 kb on + strand at 2425.854 kb on - strand at 2425.854 kb on - strand at 2425.890 kb on - strand at 2425.917 kb on + strand at 2425.925 kb on - strand at 2426.016 kb on - strand, within E.coliMinDlike) at 2426.050 kb on - strand, within E.coliMinDlike) at 2426.110 kb on - strand, within E.coliMinDlike) at 2426.362 kb on - strand, within E.coliMinDlike) at 2426.377 kb on + strand, within E.coliMinDlike) at 2426.392 kb on - strand, within E.coliMinDlike) at 2426.598 kb on - strand, within E.coliMinDlike) at 2426.724 kb on + strand at 2426.732 kb on - strand at 2426.846 kb on + strand at 2426.861 kb on - strand at 2426.910 kb on - strand at 2426.986 kb on - strand at 2426.986 kb on - strand at 2427.318 kb on + strand, within DVU2331 at 2427.366 kb on + strand, within DVU2331 at 2427.396 kb on - strand, within DVU2331 at 2427.592 kb on - strand, within DVU2331 at 2427.592 kb on - strand, within DVU2331 at 2427.703 kb on - strand, within DVU2331
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Manganese Chloride 10uM remove 2,424,953 + hypA DVU2328 0.39 +0.4 2,425,004 - hypA DVU2328 0.54 -0.0 2,425,064 + hypA DVU2328 0.71 -0.4 2,425,123 + hypA DVU2328 0.87 +0.4 2,425,152 + -0.9 2,425,813 + +0.2 2,425,854 - -0.4 2,425,854 - -0.2 2,425,890 - -0.2 2,425,917 + -0.9 2,425,925 - +0.4 2,426,016 - E.coliMinDlike) DVU2330 0.19 -0.7 2,426,050 - E.coliMinDlike) DVU2330 0.23 -0.6 2,426,110 - E.coliMinDlike) DVU2330 0.30 -0.7 2,426,362 - E.coliMinDlike) DVU2330 0.58 -0.5 2,426,377 + E.coliMinDlike) DVU2330 0.60 -0.8 2,426,392 - E.coliMinDlike) DVU2330 0.62 -1.1 2,426,598 - E.coliMinDlike) DVU2330 0.85 -0.6 2,426,724 + +0.4 2,426,732 - -0.5 2,426,846 + -0.1 2,426,861 - -0.0 2,426,910 - -1.0 2,426,986 - -0.1 2,426,986 - -0.7 2,427,318 + DVU2331 0.23 -0.9 2,427,366 + DVU2331 0.27 +0.0 2,427,396 - DVU2331 0.30 -0.1 2,427,592 - DVU2331 0.45 +0.6 2,427,592 - DVU2331 0.45 +0.5 2,427,703 - DVU2331 0.54 -0.2
Or see this region's nucleotide sequence